Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Multilayer Pva/Gelatin Nanofibrous Scaffolds Incorporated With Tanacetum Polycephalum Essential Oil and Amoxicillin for Skin Tissue Engineering Application Publisher Pubmed



Soleimandehkordi E1 ; Reisivanani V2 ; Hosseini S3, 4 ; Lorigooini Z1 ; Zvareh VA5 ; Farzan M2 ; Khorasgani EM6 ; Lozano K7 ; Abolhassanzadeh Z1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
  2. 2. Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran
  3. 3. Central Research Laboratories, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Phytochemistry Research Center, Shahid Beheshti University of Medical Science, Tehran, Iran
  5. 5. Core Research Facilities (CRF), Isfahan University of Medical Science, Isfahan, Iran
  6. 6. Department of Pathobiology, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
  7. 7. Department of Mechanical Engineering, University of Texas Rio Grande Valley, Edinburg, 78539, TX, United States

Source: International Journal of Biological Macromolecules Published:2024


Abstract

Wound infection is still an important challenge in healing of different types of skin injuries. This highlights the need for new and improved antibacterial agents with novel and different mechanisms of action. In this study, by electrospinning process Tanacetum polycephalum essential oil (EO), as a natural antibacterial and anti-inflammatory agent, along with Amoxicillin (AMX) as an antibiotic are incorporated into PVA/gelatin-based nanofiber mats individually and in combination to fabricate a novel wound dressing. Briefly, we fabricated PVA/gelatin loaded by Amoxicillin as first layer for direct contact with wound surface to protects the wound from exogenous bacteria, and then built a PVA/gelatin/Tanacetum polycephalum essential oil layer on the first layer to help cleanses the wound from infection and accelerates wound closure. Finally, PVA/gelatin layer as third layer fabricated on middle layer to guarantee desirable mechanical properties. For each layer, the electrospinning parameters were adjusted to form bead-free fibers. The morphology of fabricated nanofiber scaffolds was characterized by Fourier-transform infrared (FTIR) and scanning electron microscopy (SEM). Microscopic images demonstrated the smooth bead-free microstructures fabrication of every layer of nanofiber with a uniform fiber size of 126.888 to 136.833 nm. While, EO and AMX increased the diameter of nanofibers but there was no change in physical structure of nanofiber. The water contact angle test demonstrated hydrophilicity of nanofibers with 47.35°. Although EO and AMX had little effect on reducing hydrophilicity but nanofibers with contact angle between 51.4° until 65.4° are still hydrophilic. Multilayer nanofibers loaded by EO and AMX killed 99.99 % of both gram-negative and gram-positive bacteria in comparison with control and PVA/gelatin nanofiber. Also, in addition to confirming the non-toxicity of nanofibers, MTT results also showed the acceleration of cell proliferation. In vivo wound evaluation in mouse models showed that designed nanofibrous scaffolds could be an appropriate option for wound treatment due to their positive effect on angiogenesis, collagen deposition, granulation tissue formation, epithelialization, and wound closure. © 2024
Related Docs
View other Related Docs
1. A Promising Antibacterial Wound Dressing Made of Electrospun Poly (Glycerol Sebacate) (Pgs)/Gelatin With Local Delivery of Ascorbic Acid and Pantothenic Acid, Journal of Polymers and the Environment (2023)
2. Coaxial Electrospun Angiogenic Nanofiber Wound Dressing Containing Advanced Platelet Rich-Fibrin, International Journal of Biological Macromolecules (2022)
3. A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold, Scientific Reports (2020)
4. Core-Shell Nanofibers Containing L-Arginine Stimulates Angiogenesis and Full Thickness Dermal Wound Repair, International Journal of Pharmaceutics (2024)
5. Physical and Biological Properties of Blend-Electrospun Polycaprolactone/Chitosan-Based Wound Dressings Loaded With N-Decyl-N, N-Dimethyl-1-Decanaminium Chloride: An in Vitro and in Vivo Study, Journal of Biomedical Materials Research - Part B Applied Biomaterials (2020)
6. Preparation and Characterization of an Antibacterial Cmc/Pcl Hydrogel Films Containing Cip/Cur: In Vitro and in Vivo Evaluation of Wound Healing Activity, International Journal of Biological Macromolecules (2024)
7. Evaluation of Sustained Ciprofloxacin Release of Biodegradable Electrospun Gelatin/Poly(Glycerol Sebacate) Mat Membranes for Wound Dressing Applications, Asia-Pacific Journal of Chemical Engineering (2018)
8. A Bifunctional Electrospun Nanocomposite Wound Dressing Containing Surfactin and Curcumin: In Vitro and in Vivo Studies, Materials Science and Engineering C (2021)
Experts (# of related papers)
View all Related Experts
Jaleh Varshosaz (32)
Mohammad Rafienia (32)
Other Related Docs
9. Antimicrobial Synthetic and Natural Polymeric Nanofibers As Wound Dressing: A Review, Advanced Engineering Materials (2022)
10. Preparation and Evaluation of Poly Glycerol Sebacate/Poly Hydroxy Butyrate Core-Shell Electrospun Nanofibers With Sequentially Release of Ciprofloxacin and Simvastatin in Wound Dressings, Polymers for Advanced Technologies (2018)
11. Pdgf and Vegf-Releasing Bi-Layer Wound Dressing Made of Sodium Tripolyphosphate Crosslinked Gelatin-Sponge Layer and a Carrageenan Nanofiber Layer, International Journal of Biological Macromolecules (2023)
12. Rgd Peptide Grafted Polybutylene Adipate-Co-Terephthalate/Gelatin Electrospun Nanofibers Loaded With a Matrix Metalloproteinase Inhibitor Drug for Alleviating of Wounds: An In Vitro/In Vivo Study, Drug Development and Industrial Pharmacy (2020)
13. An in Vitro and in Vivo Study of Pcl/Chitosan Electrospun Mat on Polyurethane/Propolis Foam As a Bilayer Wound Dressing, Biomaterials Advances (2022)
14. Clinical Assessment of Novel Nanofibrous Dressings for Cutaneous Leishmaniasis: A Trial-Based Study, Polymers for Advanced Technologies (2024)
15. A Novel Wound Dressing Nanofiber With Anti-Inflammatory and Anti-Bacterial Drugs Release for Skin Wound Healing, Journal of Isfahan Medical School (2019)
16. Novel Electrospun Polyvinyl Alcohol/Chitosan/Polycaprolactone-Diltiazem Hydrochloride Nanocomposite Membranes for Wound Dressing Applications, Emergent Materials (2024)
17. Degradable and Biocompatible Nanofibrous Scaffold Incorporating a Natural Cell Culture Medium for Skin Tissue Engineering, Physica Scripta (2024)
18. Recent Advances in Electrospun Protein Fibers/Nanofibers for the Food and Biomedical Applications, Advances in Colloid and Interface Science (2023)
19. Electrospun Polyurethane-Gelatin Composite: A New Tissue-Engineered Scaffold for Application in Skin Regeneration and Repair of Complex Wounds, ACS Biomaterials Science and Engineering (2020)
20. Keratin- and Vegf-Incorporated Honey-Based Sponge-Nanofiber Dressing: An Ideal Construct for Wound Healing, ACS Applied Materials and Interfaces (2023)
21. An in Vitro and in Vivo Study of Electrospun Polyvinyl Alcohol/Chitosan/Sildenafil Citrate Mat on 3D-Printed Polycaprolactone Membrane As a Double Layer Wound Dressing, International Journal of Biological Macromolecules (2024)
22. Acceleration of Wound Healing in Rats by Modified Lignocellulose Based Sponge Containing Pentoxifylline Loaded Lecithin/Chitosan Nanoparticles, Gels (2022)
23. Electrosprayed Cefazolin-Loaded Niosomes Onto Electrospun Chitosan Nanofibrous Membrane for Wound Healing Applications, Journal of Biomedical Materials Research - Part B Applied Biomaterials (2022)
24. Nature-Inspired Healing: Biomimetic Nanomaterials for Advanced Wound Management, Materials Today Sustainability (2024)
25. Enhanced Wound Regeneration by Pgs/Pla Fiber Dressing Containing Platelet-Rich Plasma: An in Vitro Study, Scientific Reports (2024)
26. A Hybrid 3D-Printed and Electrospun Bilayer Pharmaceutical Membrane Based on Polycaprolactone/Chitosan/Polyvinyl Alcohol for Wound Healing Applications, International Journal of Biological Macromolecules (2024)
27. An Antibacterial Multi-Layered Scaffold Fabricated by 3D Printing and Electrospinning Methodologies for Skin Tissue Regeneration, International Journal of Pharmaceutics (2023)
28. Preparation of a Biomimetic Bi-Layer Chitosan Wound Dressing Composed of A-Prf/Sponge Layer and L-Arginine/Nanofiber, Carbohydrate Polymers (2022)
29. Fabrication and Characterization of a Novel Wound Dressing With Clindamycin Loaded Pva Nanoparticles for Acne Treatment, Fibers and Polymers (2022)
30. Platelet Rich Fibrin Containing Nanofibrous Dressing for Wound Healing Application: Fabrication, Characterization and Biological Evaluations, Biomaterials Advances (2022)
31. Asymmetric Tri-Layer Sponge-Nanofiber Wound Dressing Containing Insulin-Like Growth Factor-1 and Multi-Walled Carbon Nanotubes for Acceleration of Full-Thickness Wound Healing, Biomaterials Advances (2023)
32. Delivery of Antibacterial Agents for Wound Healing Applications Using Polysaccharide-Based Scaffolds, Journal of Drug Delivery Science and Technology (2023)
33. A Multifunctional Nanocomposite Spray Dressing of Kappa-Carrageenan-Polydopamine Modified Zno/L-Glutamic Acid for Diabetic Wounds, Materials Science and Engineering C (2020)
34. A Double-Layer Cellulose/Pectin-Soy Protein Isolate-Pomegranate Peel Extract Micro/Nanofiber Dressing for Acceleration of Wound Healing, International Journal of Biological Macromolecules (2024)
35. Antibacterial Self-Healing Bilayer Dressing for Epidermal Sensors and Accelerate Wound Repair, Carbohydrate Polymers (2023)
36. Alginate Hydrogel Enriched With Ambystoma Mexicanum Epidermal Lipoxygenase-Loaded Pectin Nanoparticles for Enhanced Wound Healing, Journal of Biomaterials Applications (2020)
37. Nanofibrous Scaffolds With Biomimetic Composition for Skin Regeneration, Applied Biochemistry and Biotechnology (2019)
38. Fabrication and Characterization of Glycerol/Chitosan/Polyvinyl Alcohol-Based Transparent Hydrogel Films Loaded With Silver Nanoparticles for Antibacterial Wound Dressing Applications, Advanced Biomedical Research (2021)
39. Comparing the Wound Healing Effect of a Controlled Release Wound Dressing Containing Curcumin/Ciprofloxacin and Simvastatin/Ciprofloxacin in a Rat Model: A Preclinical Study, Journal of Biomedical Materials Research - Part A (2022)
40. In Vitro and in Vivo Performance of a Propolis-Coated Polyurethane Wound Dressing With High Porosity and Antibacterial Efficacy, Colloids and Surfaces B: Biointerfaces (2019)
41. Platelet Rich Fibrin Containing Nanofibrous Dressing for Wound Healing Application: Fabrication, Characterization and Biological Evaluations, Materials Science and Engineering C (2021)
42. Cytocompatibility and Antibacterial Properties of Coaxial Electrospun Nanofibers Containing Ciprofloxacin and Indomethacin Drugs, Polymers (2022)
43. Pmma Nanofibers Containing Keplerate-Type Polyoxometalate and Metronidazole: Preparation and Wound-Healing Effect in a Rat Model, Journal of Drug Delivery Science and Technology (2022)
44. Synthesis of Polyurethane/Hyaluronic Acid/Royal Jelly Electrospun Scaffold and Evaluating Its Properties for Wound Healing, Journal of Mazandaran University of Medical Sciences (2021)
45. Preparation and Characterization of Trimethyl Chitosan /Hyaluronic Acid Composite Encapsulated With Ll37 to Promote Wound Healing in an Animal Model, International Journal of Peptide Research and Therapeutics (2023)
46. The Effects of Nanoemulsion Cream Containing Lavender Essential Oil and Licorice Extract on Healing of Deep Skin Wound in Rats, Journal of Isfahan Medical School (2019)
47. Casein Release and Characterization of Electrospun Nanofibres for Cartilage Tissue Engineering, Bulletin of Materials Science (2022)
48. Characterization of Poly(Ε-Caprolactone)/Extracellular Matrix Nanofibers Composite Scaffold for Tissue Engineering, Journal of Isfahan Medical School (2019)
49. Preparation and in Vitro Evaluation of Vancomycin Loaded Montmorillonite-Sodium Alginate Topical Gel for Wound Infection, Brazilian Journal of Pharmaceutical Sciences (2022)
50. A High-Efficient Antibacterial and Biocompatible Polyurethane Film With Ag@Rgo Nanostructures Prepared by Microwave-Assisted Method: Physicochemical and Dermal Wound Healing Evaluation, Heliyon (2023)