Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Enhanced Wound Regeneration by Pgs/Pla Fiber Dressing Containing Platelet-Rich Plasma: An in Vitro Study Publisher Pubmed



Heydari P1, 2 ; Zargar Kharazi A2, 3 ; Shariati L2, 4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Materials Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran
  2. 2. Applied Physiology Research Center, Isfahan, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Biomaterials Nanotechnology and Tissue Engineering Faculty, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Scientific Reports Published:2024


Abstract

Novel wound dressings with therapeutic effects are being continually designed to improve the wound healing process. In this study, the structural, chemical, physical, and biological properties of an electrospun poly glycerol sebacate/poly lactide acid/platelet-rich plasma (PGS/PLA-PRP) nanofibers were evaluated to determine its impacts on in vitro wound healing. Results revealed desirable cell viability in the Fibroblast (L929) and macrophage (RAW-264.7) cell lines as well as human umbilical vein endothelial cells (HUVEC). Cell migration was evident in the scratch assay (L929 cell line) so that it promoted scratch contraction to accelerate in vitro wound healing. Moreover, addition of PRP to the fiber structure led to enhanced collagen deposition (~ 2 times) in comparison with PGS/PLA scaffolds. While by addition PRP to PGS/PLA fibers not only decreased the expression levels of pro-inflammatory cytokines (IL-6 and TNF-α) in RAW-264.7 cells but also led to significantly increased levels of cytokine (IL-10) and the growth factor (TGF-β), which are related to the anti-inflammatory phase (M2 phenotype). Finally, PGS/PLA-PRP was found to induce a significant level of angiogenesis by forming branching points, loops, and tubes. Based on the results obtained, the PGS/PLA-PRP dressing developed might be a promising evolution in skin tissue engineering ensuring improved wound healing and tissue regeneration. © The Author(s) 2024.
Related Docs
View other Related Docs
1. Coaxial Electrospun Pgs/Pcl and Pgs/Pgs-Pcl Nanofibrous Membrane Containing Platelet-Rich Plasma for Skin Tissue Engineering, Journal of Biomaterials Science, Polymer Edition (2024)
2. Sustained Release Investigation of Curcumin and Ciprofloxacin on Coaxial Electrospun Nanocomposite Scaffold of Poly (3-Hydroxybutyrate)-Poly Glycerol Sebacate: An Approach for Skin Regeneration, Emergent Materials (2024)
3. Preparation and Characterization of an Antibacterial Cmc/Pcl Hydrogel Films Containing Cip/Cur: In Vitro and in Vivo Evaluation of Wound Healing Activity, International Journal of Biological Macromolecules (2024)
4. Carboxymethyl Cellulose/Sodium Alginate Hydrogel With Anti-Inflammatory Capabilities for Accelerated Wound Healing; in Vitro and in Vivo Study, European Journal of Pharmacology (2024)
5. Co-Release of Nitric Oxide and L-Arginine From Poly (Β-Amino Ester)-Based Adhesive Reprogram Macrophages for Accelerated Wound Healing and Angiogenesis in Vitro and in Vivo, Biomaterials Advances (2024)
6. Comparing the Wound Healing Effect of a Controlled Release Wound Dressing Containing Curcumin/Ciprofloxacin and Simvastatin/Ciprofloxacin in a Rat Model: A Preclinical Study, Journal of Biomedical Materials Research - Part A (2022)
7. Fabrication of a Tri-Layer Scaffold With Dual Release of Heparin and Prp for Tissue Engineering of Small‐Diameter Blood Vessels, International Journal of Polymeric Materials and Polymeric Biomaterials (2024)
8. A Promising Antibacterial Wound Dressing Made of Electrospun Poly (Glycerol Sebacate) (Pgs)/Gelatin With Local Delivery of Ascorbic Acid and Pantothenic Acid, Journal of Polymers and the Environment (2023)
Experts (# of related papers)
View all Related Experts
Mohammad Rafienia (38)
Jaleh Varshosaz (33)
Other Related Docs
9. Blood Compatibility and Cell Response Improvement of Poly Glycerol Sebacate/Poly Lactic Acid Scaffold for Vascular Graft Applications, Journal of Biomedical Materials Research - Part A (2021)
10. A Novel Wound Dressing Nanofiber With Anti-Inflammatory and Anti-Bacterial Drugs Release for Skin Wound Healing, Journal of Isfahan Medical School (2019)
11. Polyglycerol Sebacate/Polycaprolactone/Reduced Graphene Oxide Composite Scaffold for Myocardial Tissue Engineering, Heliyon (2024)
12. Synthetic-Based Blended Electrospun Scaffolds in Tissue Engineering Applications, Journal of Materials Science (2022)
13. Preparation and Characterization of Carboxymethyl Cellulose/Polyethylene Glycol Films Containing Bromelain/Curcumin: In Vitro Evaluation of Wound Healing Activity, Polymer Engineering and Science (2024)
14. Comparison of Physical, Mechanical and Biological Effects of Leucocyte-Prf and Advanced-Prf on Polyacrylamide Nanofiber Wound Dressings: In Vitro and in Vivo Evaluations, Biomaterials Advances (2022)
15. Evaluation of Sustained Ciprofloxacin Release of Biodegradable Electrospun Gelatin/Poly(Glycerol Sebacate) Mat Membranes for Wound Dressing Applications, Asia-Pacific Journal of Chemical Engineering (2018)
16. Promoting Keratocyte Stem Like Cell Proliferation and Differentiation by Aligned Polycaprolactone-Silk Fibroin Fibers Containing Aloe Vera, Biomaterials Advances (2022)
17. Core-Shell Nanofibers Containing L-Arginine Stimulates Angiogenesis and Full Thickness Dermal Wound Repair, International Journal of Pharmaceutics (2024)
18. Promoting Neural Cell Proliferation and Differentiation by Incorporating Lignin Into Electrospun Poly(Vinyl Alcohol) and Poly(Glycerol Sebacate) Fibers, Materials Science and Engineering C (2019)
19. Preparation of a Biomimetic Bi-Layer Chitosan Wound Dressing Composed of A-Prf/Sponge Layer and L-Arginine/Nanofiber, Carbohydrate Polymers (2022)
20. Platelet Rich Fibrin Containing Nanofibrous Dressing for Wound Healing Application: Fabrication, Characterization and Biological Evaluations, Biomaterials Advances (2022)
21. In Vitro Comparison Study of Plasma Treated Bilayer Pgs/Pcl and Pgs/Pla Scaffolds for Vascular Tissue Engineering, Fibers and Polymers (2022)
22. Antimicrobial Synthetic and Natural Polymeric Nanofibers As Wound Dressing: A Review, Advanced Engineering Materials (2022)
23. Electrospun Pgs/Pcl Nanofibers: From Straight to Sponge and Spring-Like Morphology, Polymers for Advanced Technologies (2020)
24. Preparation and Evaluation of Poly Glycerol Sebacate/Poly Hydroxy Butyrate Core-Shell Electrospun Nanofibers With Sequentially Release of Ciprofloxacin and Simvastatin in Wound Dressings, Polymers for Advanced Technologies (2018)
25. Platelet Rich Fibrin Containing Nanofibrous Dressing for Wound Healing Application: Fabrication, Characterization and Biological Evaluations, Materials Science and Engineering C (2021)
26. Design and Fabrication of Poly (Glycerol Sebacate)-Based Fibers for Neural Tissue Engineering: Synthesis, Electrospinning, and Characterization, Polymers for Advanced Technologies (2019)
27. Poly (Glycerol Sebacate) and Polyhydroxybutyrate Electrospun Nanocomposite Facilitates Osteogenic Differentiation of Mesenchymal Stem Cells, Journal of Drug Delivery Science and Technology (2021)
28. Electrospun Nano-Fibers for Biomedical and Tissue Engineering Applications: A Comprehensive Review, Materials (2020)
29. The Effect of Different Solvent Systems on Physical Properties of Electrospun Poly(Glycerol Sebacate)/Poly(E-Caprolactone) Blend, Polymer-Plastics Technology and Materials (2022)
30. Electroconductive Nanofiber/Myocardium Gel Scaffolds Applicable for Myocardial Infarction Therapy, ACS Applied Polymer Materials (2024)
31. Assessing Physicochemical, Mechanical, and in Vitro Biological Properties of Polycaprolactone/Poly(Glycerol Sebacate)/Hydroxyapatite Composite Scaffold for Nerve Tissue Engineering, Materials Chemistry and Physics (2022)
32. Fabrication and Characterization of a 3D Scaffold Based on Elastomeric Poly-Glycerol Sebacate Polymer for Heart Valve Applications, Journal of Manufacturing Processes (2023)
33. Synthetic Electrospun Nanofibers As a Supportive Matrix in Osteogenic Differentiation of Induced Pluripotent Stem Cells, Journal of Biomaterials Science, Polymer Edition (2022)
34. An in Vitro and in Vivo Study of Electrospun Polyvinyl Alcohol/Chitosan/Sildenafil Citrate Mat on 3D-Printed Polycaprolactone Membrane As a Double Layer Wound Dressing, International Journal of Biological Macromolecules (2024)
35. Fabrication and Characterization of Metformin-Loaded Plga/Collagen Nanofibers for Modulation of Macrophage Polarization for Tissue Engineering and Regenerative Medicine, BMC Biotechnology (2023)
36. Delivery of Antibacterial Agents for Wound Healing Applications Using Polysaccharide-Based Scaffolds, Journal of Drug Delivery Science and Technology (2023)
37. Alginate-Based Nanocomposite Incorporating Chitosan Nanoparticles: A Dual-Drug Delivery System for Infection Control and Wound Regeneration, Journal of Drug Delivery Science and Technology (2025)
38. A Propolis Enriched Polyurethane-Hyaluronic Acid Nanofibrous Wound Dressing With Remarkable Antibacterial and Wound Healing Activities, International Journal of Biological Macromolecules (2020)
39. Preparation and Characterization of Poly (Lactic-Co-Glycolic Acid) Nanofibers Containing Simvastatin Coated With Hyaluronic Acid for Using in Periodontal Tissue Engineering, Biotechnology Progress (2021)
40. Adipose-Derived Stem Cells Growth and Proliferation Enhancement Using Poly (Lactic-Co-Glycolic Acid) (Plga)/Fibrin Nanofiber Mats, Journal of Applied Biotechnology Reports (2021)
41. A Nanofibrous Bilayered Scaffold for Tissue Engineering of Small-Diameter Blood Vessels, Polymers for Advanced Technologies (2018)
42. Multilayer Pva/Gelatin Nanofibrous Scaffolds Incorporated With Tanacetum Polycephalum Essential Oil and Amoxicillin for Skin Tissue Engineering Application, International Journal of Biological Macromolecules (2024)
43. An in Vitro and in Vivo Study of Pcl/Chitosan Electrospun Mat on Polyurethane/Propolis Foam As a Bilayer Wound Dressing, Biomaterials Advances (2022)
44. In Vitro Hemocompatibility and Cytocompatibility of a Three-Layered Vascular Scaffold Fabricated by Sequential Electrospinning of Pcl, Collagen, and Plla Nanofibers, Journal of Biomaterials Applications (2016)
45. Physical and Biological Properties of Blend-Electrospun Polycaprolactone/Chitosan-Based Wound Dressings Loaded With N-Decyl-N, N-Dimethyl-1-Decanaminium Chloride: An in Vitro and in Vivo Study, Journal of Biomedical Materials Research - Part B Applied Biomaterials (2020)
46. Keratin- and Vegf-Incorporated Honey-Based Sponge-Nanofiber Dressing: An Ideal Construct for Wound Healing, ACS Applied Materials and Interfaces (2023)
47. Improved Wound Healing of Diabetic Foot Ulcers Using Human Placenta-Derived Mesenchymal Stem Cells in Gelatin Electrospun Nanofibrous Scaffolds Plus a Platelet-Rich Plasma Gel: A Randomized Clinical Trial, International Immunopharmacology (2021)
48. Novel Electrospun Polyvinyl Alcohol/Chitosan/Polycaprolactone-Diltiazem Hydrochloride Nanocomposite Membranes for Wound Dressing Applications, Emergent Materials (2024)
49. A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold, Scientific Reports (2020)
50. Synthesis of Polyurethane/Hyaluronic Acid/Royal Jelly Electrospun Scaffold and Evaluating Its Properties for Wound Healing, Journal of Mazandaran University of Medical Sciences (2021)