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A Hybrid 3D-Printed and Electrospun Bilayer Pharmaceutical Membrane Based on Polycaprolactone/Chitosan/Polyvinyl Alcohol for Wound Healing Applications Publisher Pubmed



Mehdikhani M1, 2 ; Yilgor P3 ; Poursamar SA4 ; Etemadi N5, 6 ; Gokyer S3 ; Navid S7 ; Farzan M8 ; Farzan M8 ; Babaei M4 ; Rafienia M10
Authors
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Authors Affiliations
  1. 1. Department of Biomedical Engineering, Faculty of Engineering, University of Isfahan, Isfahan, Iran
  2. 2. Research and Technology Center for International Scientific Studies and Collaboration (CISSC), Ministry of Science, Tehran, Iran
  3. 3. Department of Biomedical Engineering, Faculty of Engineering, Ankara University, Ankara, Turkey
  4. 4. Department of Biomaterials, Nanotechnology and Tissue Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  5. 5. Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon, 16419, South Korea
  6. 6. Department of Intelligent Precision Healthcare Convergence, Sungkyunkwan University, Suwon, 16419, South Korea
  7. 7. Core Research Facilities, Isfahan University of Medical Sciences, Isfahan, Iran
  8. 8. Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran
  9. 9. Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
  10. 10. Biosensor Research Center (BRC), Isfahan University of Medical Sciences (IUMS), Isfahan, Iran

Source: International Journal of Biological Macromolecules Published:2024


Abstract

Skin injuries resulting from physical trauma pose significant health risks, necessitating advanced wound care solutions. This investigation introduces an innovative bilayer wound dressing composed of 3D-printed propolis-coated polycaprolactone (PCL/PP) and an electrospun composite of polyvinyl alcohol, chitosan, polycaprolactone, and diltiazem (PVA/CTS/PCL/DTZ). SEM analysis revealed a bilayer structure with 89.23 ± 51.47 % porosity and uniformly distributed nanofibers. The scaffold tensile strength, with pore sizes of 100, 300, and 500 μm, was comparable to native skin. However, smaller pore sizes reduced water vapor transmission from 4211.59 ± 168.53 to 2358.49 ± 203.63 g/m2. The incorporation of DTZ lowered the contact angle to 35.23 ± 3.65°, while the addition of PCL reduced the degradation rate and modulated the release of DTZ by approximately 50 %. Moreover, lower pH increased the degradation rate and decreased swelling. The inclusion of propolis enhanced antibacterial activity, and 10 % DTZ promoted the viability, proliferation, and migration of fibroblasts and adipose-derived stem cells. However, increasing DTZ concentration to 12 % reduced cell viability. In vivo tests on rats demonstrated effective wound healing and anti-inflammatory properties of the bilayer samples. Regarding the aforementioned results, the PCL/PP-PVA/CTS/PCL/DTZ (10 % w/w) bilayer wound dressing is a promising candidate for wound healing applications. © 2024
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