Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Antiviral Activity of Punica Granatum Species Pleniflora, Saveh Black Leather, and Sweet Alak Against Herpes Simplex Virus Type 1 Publisher



Gavanji S1 ; Baghshahi H2 ; Bakhtari A3 ; Hamami Chamgordani Z4 ; Badripour N2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Iran
  2. 2. Barij Medicinal Plants Research Center, Kashan, Iran
  3. 3. Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
  4. 4. Department of Adult Health Nursing, Faculty of Nursing and Midwifery, Isfahan University of Medical Sciences, Isfahan, Iran

Source: New Armenian Medical Journal Published:2024


Abstract

Background: Herpes simplex virus-1, commonly known as oral herpes, is a highly contagious viral infection in humans. Various therapies and clinical management strategies have treated Herpes simplex virus-1 infection, but drug resistance is a concern, which has sparked an obsession with herpes simplex virus therapy. Therefore, interest in herbal medications with antiviral properties has increased. This research aimed to investigate the antiviral activity of pomegranate flower extracts on herpes simplex virus-1 type 1 in Vero cells under in vitro conditions. Material andMethods: This study evaluated the anti-herpetic effect of pomegranate flower extracts, and the plaque reduction assay was performed on Vero cells. For cytotoxicity determination of pomegranate flower extracts, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide was used. The total flavonoid and phenolic contents of three varieties of Punica granatum (P. granatum) were measured based on gallic acid (mg/g) and rutin equivalents (mg/g), respectively. Results: Pomegranate flower extracts had no cytotoxic impact at doses ranging from 80 to 140 μg/ml. Our study revealed that pomegranate flower extracts prevented the growth of viral plaques, and the IC50 values of three P. granatum species—pleniflora, Saveh Black Leather, and Sweet Alak—were 109.63, 131.24, and 128.87 μg/ml, respectively. Also, evaluation of total phenolic and flavonoid content showed that P. granatum var. pleniflora (Golnare farsi) had the highest total phenolic and flavonoid content (17.8 mg/g of gallic acid and 2.2 mg/g of rutin equivalents, respectively). Conclusion: The pomegranate flower extracts have an inhibitory impact on herpes simplex virus-1 and could be used as an anti-HSV-1 agent in further investigation. © 2024, Yerevan State Medical University. All rights reserved.
Related Docs
View other Related Docs
1. Design, Formulation, and Evaluation of a Herbal Gel Contains Melissa, Sumac, Licorice, Rosemary, and Geranium for Treatment of Recurrent Labial Herpes Infections, Dental Research Journal (2018)
2. Antiviral and Cytotoxic Evaluation of Coumarins From Prangos Ferulacea, Pharmaceutical Biology (2014)
3. Cytotoxicity Effects of Ethanolic Extract of Punica Granatum Var. Pleniflora on Mcf-7 Compared With L929 Cells, New Armenian Medical Journal (2023)
4. Anti-Viral Evaluation of Sesquiterpene Coumarins From Ferula Assa-Foetida Against Hsv-1, Iranian Journal of Pharmaceutical Research (2014)
5. Cytotoxic Evaluation of Volatile Oil From Descurainia Sophia Seeds on Mcf-7 and Hela Cell Lines, Research in Pharmaceutical Sciences (2015)
6. Is Consumption of Pomegranate Supplementation Effective on Oxidative Stress Biomarkers Including Mda, Ox-Ldl, Pox 1, Gpx, Tac, and Tbras? a Systematic Review and Meta-Analysis of Randomized Controlled Trials, Current Problems in Cardiology (2023)
7. Cutaneous Adverse Reactions to Herbal Medicines, New Armenian Medical Journal (2023)
8. Role of Peroxisome Proliferator-Activated Receptor Alpha and Gamma in Antiangiogenic Effect of Pomegranate Peel Extract, Iranian Journal of Basic Medical Sciences (2016)
Experts (# of related papers)
View all Related Experts
Behzad Zolfaghari (3)
Bahram Nasr Esfahani (2)
Other Related Docs
9. A Narrative Literature Review on Traditional Medicine Options for Treatment of Corona Virus Disease 2019 (Covid-19), Complementary Therapies in Clinical Practice (2020)
10. Antiviral Potential of Melissa Officinalis L.: A Literature Review, Nutrition and Metabolic Insights (2023)
11. The Significant Impact of Apigenin on Different Aspects of Autoimmune Disease, Inflammopharmacology (2018)
12. Antiviral Activity of Pistacia Atlantica Subsp. Kurdica Extract Against Herpes Simplex Virus Type 1; [Pistacia Atlantica Subsp.'Nin Antiviral Etkinligi Herpes Simplex Virusu Tip 1'E Karsi Kurdica Ekstrakti], Gazi Medical Journal (2024)
13. Inhibitory Activity of Avicennia Marina, a Medicinal Plant in Persian Folk Medicine, Against Hiv and Hsv, Iranian Journal of Pharmaceutical Research (2013)
14. Preventive Effect of Three Pomegranate (Punica Granatum L.) Seeds Fractions on Cerulein-Induced Acute Pancreatitis in Mice, International Journal of Preventive Medicine (2014)
15. Pharmacotherapeutic Potential of Pomegranate in Age-Related Neurological Disorders, Frontiers in Aging Neuroscience (2022)
16. Inhibitory Effect and Structure-Activity Relationship of Some Biginelli-Type Pyrimidines Against Hsv-1, Medicinal Chemistry Research (2013)
17. Inhibition of Herpes Simplex Virus Type 1 Replication by Novel Hsa-Mir-7704 in Vitro, Research in Pharmaceutical Sciences (2019)
18. Design, Formulation and Evaluation of an Oral Gel From Punica Granatum Flower Extract for the Treatment of Recurrent Aphthous Stomatitis, Advanced Pharmaceutical Bulletin (2016)
19. The Effect of Root, Shoot and Seed Extracts of the Iranian Thymus L. (Family: Lamiaceae) Species on Hiv-1 Replication and Cd4 Expression, Cell Journal (2016)
20. The Effect of Mirna Mimic Hsa-Mir-7704 on In-Vitro Replication of Herpes Simplex Virus Type 1, Journal of Isfahan Medical School (2018)