Association Between Hla-Dqb1 Alleles and Ham/Tsp Patients in Khorasan Province



Tafreshian AR¹ ; Etemadi M² ; Faridhosseini R³ ; Salhi M⁴ ; Afshari JT¹ Show Affiliations
Source: Iranian Journal of Basic Medical Sciences Published:2014

Abstract

Objective(s): HTLVI-1 is the first human retrovirus with limited endemic regions in the world. The epidemiological studies have shown that the genetic background and immune response to the virus have a significant role in HTLV-I-associated diseases. Among the genes are involved in HTLV-I infection, the role of human leukocytes antigen (HLA) have been studied in different population. In the present study we examined the association between HLA-DQB1 alleles and HTLV-I infection in HAM/TSP patients, HTLV-I carriers and healthy controls in north east of Iran, Mashhad.; Materials and Methods: The blood samples of 16 patients with HAM/TSP, 20 HTLV-1 carriers, and 30 healthy individuals were taken and DNA was extracted by salting out method. HLA-DQB1 typing was performed using PCR-SSP method and the frequency of HLA-DQB1 alleles were compared by Fischer Exact Test.; Results: There was a significant difference between HAM/TSP patients and healthy controls in the frequency of HLA-DQB1*07 (P=0.004, RR=7). Furthermore, we found that possession of HLA- DQB1*02 or HLA-DQB1*05 increased the risk of disease 1.5 times.; Conclusion: The data presented here suggest that both HLA-DQB1*07 and HLA-DQB1*06 are associated with disease development. © 2014, Mashhad University of Medical Sciences. All Rights Reserved.