Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Comparison of Poly(Lactic-Co-Glycolic Acid) and Poly(Lactic-Co-Glycolic Acid)/Hyaluronic Acid Based Scaffolds for Chondrogenesis of Human Adipose-Derived Stem Cells by Avocado/Soybean Unsaponifiables Publisher



Hashemibeni B1 ; Sharifian Z2 ; Valiani A1 ; Dortaj H3 ; Rarani MZ2 ; Mardani M1 ; Pourentezari M4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Anatomy, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
  3. 3. Department of Tissue Engineering and Applied Cell Science, Shiraz University of Applied Medical Science and Technologies, Shiraz, Iran
  4. 4. Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Source: Journal of Kerman University of Medical Sciences Published:2021


Abstract

Background: Corporation of Hyaluronic acid (HA) with PLGA is an effective way to potentially enhance chondrogenesis. The aim of this study was to use HA macroporous biodegradable poly(lactic acid-co-glycolic acid) [PLGA] scaffold to enhance the attachment, proliferation and differentiation of chondrocytes for cartilage tissue engineering and articular cartilage regeneration of human adipose derived stem cells (hADSCs) in the presence of avocado/soybean unsaponifible (ASU). Methods: The PLGA and PLGA/HA scaffolds were prepared and hADSCs were cultured separately on the scaffolds and 14 days after differentiation, chondrogenic genes in each scaffold evaluated using real time PCR and cell viability examined by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The viability and proliferation of cells in-group of PLGA significantly decreased in comparison with the control (P=0.002) and PLGA/HA (P=0.013) groups. The expression of (SOX9), Aggrecan (AGG), and Collagen type II (Col II) genes was significantly higher in the PLGA and PLGA/HA groups compared to the control group (P≥0.05). The gene expression of SOX9 (P=0.003) and AGG (P=0.009) was significantly higher in the PLGA/HA groups compared to the PLGA group. The results of real time PCR showed that collagen type X (Col X) gene expression in the PLGA group, was significantly higher than the control and PLGA/HA groups (P=0.000). Conclusion: The corporation of HA with PLGA is an effective way to potentially enhance chondrogenesis and articular cartilage regeneration of hADSCs in the presence of avocado/soybean unsaponifiables (ASU). © 2021, Kerman University of Medical Sciences. All rights reserved.
Related Docs
View other Related Docs
1. Comparison of Tgf-Β3 and Avocado/Soybean Unsaponifiable on Chondrogenesis of Human Adipose-Derived Stem Cells on Poly (Lactic-Co-Glycolic) Acid/ Hyaluronic Acid Hybrid Scaffold, Iranian Journal of Basic Medical Sciences (2020)
2. Evaluation Avocado Soybean Unsaponifiables Loaded in Poly (Lactic-Co-Glycolic) Acid/Avocado Soybean Unsaponifiables-Fibrin Nanoparticles Scaffold (New Delivery System) Is an Effective Factor for Tissue Engineering, Advanced Biomedical Research (2021)
3. Impact of Fibrin on the Chondrogenic Avocado Soybean Unsaponifiables on Poly (Lactic-Co-Glycolic) Acid Scaffold, Biointerface Research in Applied Chemistry (2021)
4. Investigation and Comparison of the Effect of Tgf-Β3, Kartogenin and Avocado/Soybean Unsaponifiables on the In-Vitro and In-Vivo Chondrogenesis of Human Adipose-Derived Stem Cells on Fibrin Scaffold, Iranian Journal of Pharmaceutical Research (2021)
5. Effect of Transforming Growth Factor-Β3 and Bone Morphogenetic Protein-6 Growth Factors on Chondrogenic Differentiation of Adipose-Derived Stem Cells in Alginate Scaffold, Journal of Isfahan Medical School (2010)
6. Chondrogenesis of Mesenchymal Stromal Cells on the 3D Printed Polycaprolactone/Fibrin/Decellular Cartilage Matrix Hybrid Scaffolds in the Presence of Piascledine, Journal of Biomaterials Science, Polymer Edition (2024)
7. Effects of Avocado/Soybean on the Chondrogenesis of Human Adipose-Derived Stem Cells Cultured on Polylactic-Co-Glycolic Acid/Fibrin Hybrid Scaffold, Journal of Applied Biotechnology Reports (2019)
8. Effect of Piasclidin on Induction of Chondrogenesis by Human Adipose-Derived Stem Cells in Fibrin Scaffold, Journal of Isfahan Medical School (2016)
Experts (# of related papers)
View all Related Experts
Batool Hashemibeni (44)
Saeed Karbasi (33)
Other Related Docs
9. The Effects of Fibrin–Icariin Nanoparticle Loaded in Poly (Lactic‑Co‑Glycolic) Acid Scaffold As a Localized Delivery System on Chondrogenesis of Human Adipose‑Derived Stem Cells, Advanced Biomedical Research (2020)
10. The Effect of Bmp-6 Growth Factor on Differentiation of Adipose-Derived Stem Cells Into Chondrocyte in Pellet Culture System, Journal of Isfahan Medical School (2009)
11. Chondrogenic Activity of Two Herbal Products; Pomegranate Fruit Extract and Avocado/Soybean Unsaponifiable, Research in Pharmaceutical Sciences (2020)
12. Comparison of the Efficacy of Piascledine and Transforming Growth Factor Β1 on Chondrogenic Differentiation of Human Adipose-Derived Stem Cells in Fibrin and Fibrin-Alginate Scaffolds, Iranian Journal of Basic Medical Sciences (2018)
13. A Novel Nano-Composite Scaold for Cartilage Tissue Engineering, Scientia Iranica (2018)
14. Incorporation of Chitosan Nanoparticles Into Silk Fibroin-Based Porous Scaffolds: Chondrogenic Differentiation of Stem Cells, International Journal of Polymeric Materials and Polymeric Biomaterials (2016)
15. Comparing the Effect of Silk Fibroin-Based Scaffolds on Differentiation of Rabbit Chondrocytes, Journal of Isfahan Medical School (2014)
16. Effects of Fgf-2 on Human Adipose Tissue Derived Adult Stem Cells Morphology and Chondrogenesis Enhancement in Transwell Culture, Biochemical and Biophysical Research Communications (2012)
17. Comparison of Fibrin and Plga/Fibrin Scaffolds for Chondrogenesis of Human Adipose Derived Stem Cells by Icariin, Journal of Kerman University of Medical Sciences (2020)
18. Induction of Chondrogenic Differentiation of Human Adipose-Derived Stem Cells With Tgf-Β3 in Pellet Culture System, Iranian Journal of Basic Medical Sciences (2008)
19. Study of Carbon Nano-Tubes Effects on the Chondrogenesis of Human Adipose Derived Stem Cells in Alginate Scaffold, International Journal of Preventive Medicine (2014)
20. Comparison Between Chondrogenic Markers of Differentiated Chondrocytes From Adipose Derived Stem Cells and Articular Chondrocytes in Vitro, Iranian Journal of Basic Medical Sciences (2013)
21. Stem Cells Therapy for Cartilage Regeneration in Clinic: Challenges and Opportunities, Cartilage: From Biology to Biofabrication (2023)
22. A Comparative Study of Articular Chondrocytes Metabolism on a Biodegradable Polyesterurethane Scaffold and Alginate in Different Oxygen Tension and Ph, IFMBE Proceedings (2009)
23. Effects of Cartilage Acellular Solubilised Ecm on Physicomechanical and Biological Properties of Polycaprolactone/Fibrin Hybrid Scaffold Fabricated by 3D-Printing and Salt-Leaching Methods, Materials Technology (2022)
24. A Comparative Study of Articular Chondrocytes Metabolism on a Biodegradable Polyesterurethane Scaffold and Alginate Beads in Different Oxygen Tension and Ph, Journal of Isfahan Medical School (2009)
25. The Effect of High Frequency Electric Field on Enhancement of Chondrogenesis in Human Adipose-Derived Stem Cells, Iranian Journal of Basic Medical Sciences (2014)
26. A Comparison Between Cell Viability of Chondrocytes on a Biodegradable Polyester Urethane Scaffold and Alginate Beads in Different Oxygen Tension and Ph, Iranian Polymer Journal (English Edition) (2005)
27. An Animal Model Study of Osteochondral Defect Repair by Human Adipose Stem Cells and Pomegranate Fruit Hydroalchoholic Extract, Avicenna Journal of Phytomedicine (2023)
28. Cartilage Tissue Formation From Human Adipose-Derived Stem Cells Via Herbal Component (Avocado/Soybean Unsaponifiables) in Scaffold-Free Culture System, Dental Research Journal (2020)
29. Herbal Remedies As Potential in Cartilage Tissue Engineering: An Overview of New Therapeutic Approaches and Strategies, Molecules (2020)
30. Application of Some Herbal Medicine Used for the Treatment of Osteoarthritis and Chondrogenesis, Traditional and Integrative Medicine (2020)
31. The Effect of Pomegranate Extract on Producing Type Ii Collagen in Differentiation of Adipose-Derived Stem Cells Into Chondrocytes, Journal of Isfahan Medical School (2018)
32. Hybrid and Composite Scaffolds Based on Extracellular Matrices for Cartilage Tissue Engineering, Tissue Engineering - Part B: Reviews (2019)
33. Comparing the Effects of Transforming Growth Factor Beta1 (Tgf-ß1) and Piascledine on the Expression of Collagen Ii, X and Aggrecan Genes in Chondrogenesis of Human Adipose-Derived Stem Cells in Fibrin Alginate Composite Scaffold, Journal of Isfahan Medical School (2016)
34. Evaluation of Pomegranate Seed Extract and Tgf-Β3 on Chondrogenic Differentiation of Human Adipose-Derived Stem Cells, Journal of Applied Biotechnology Reports (2022)
35. Comparison Between the Effect of Kartogenin and Tgfβ3 on Chondrogenesis of Human Adipose- Derived Stem Cells in Fibrin Scaffold, Bratislava Medical Journal (2017)
36. Comparison of Aggrecan Gene Expression in Chondrogenesis of Adipose-Derived Stem Cells in Pellet and Micromass Culture Systems, Tehran University Medical Journal (2018)
37. The Effects of Tgf-Β3 on the Proliferation and Function of Encapsulated Costal Cartilage Chondrocytes in Alginate Scaffold, Journal of Applied Biotechnology Reports (2019)
38. Chondrogenic Differentiation of Human Scalp Adipose-Derived Stem Cells in Polycaprolactone Scaffold and Using Freeze Thaw Freeze Method, Journal of Cellular Physiology (2018)
39. Chondrogenesis of Human Adipose-Derived Mesenchymal Stromal Cells on the [Devitalized Costal Cartilage Matrix/Poly(Vinyl Alcohol)/Fibrin] Hybrid Scaffolds, European Polymer Journal (2019)
40. Evaluation of the Effects of Glucosamine Sulfate on Poly(3- Hydroxybutyrate) -Chitosan/Carbon Nanotubes Electrospun Scaffold for Cartilage Tissue Engineering Applications, Polymer-Plastics Technology and Materials (2022)
41. Evaluation of Hydrostatic Pressure on Metabolism of the Articular Chondrocytes Seeded on Biodegradable Polyurethane As Tissue Engineering Scaffold, IFMBE Proceedings (2007)
42. Comparison of Cd44 and Cd14 Surface Markers in Adipose-Derived Stem Cells and Differentiated Chondrocytes During Chondrogenesis, Journal of Isfahan Medical School (2014)
43. Adipose-Derived Stem Cells Growth and Proliferation Enhancement Using Poly (Lactic-Co-Glycolic Acid) (Plga)/Fibrin Nanofiber Mats, Journal of Applied Biotechnology Reports (2021)
44. The Effect of Estradiol on Production of Type Ii Collagen and Aggrecan in Chondrogenic Proccess, Journal of Isfahan Medical School (2014)
45. The Effect of Platelet Rich Plasma on Chondrogenic Differentiation of Human Adipose Derived Stem Cells in Transwell Culture, Iranian Journal of Basic Medical Sciences (2013)
46. Electrospun Halloysite Nanotube Loaded Polyhydroxybutyrate-Starch Fibers for Cartilage Tissue Engineering, International Journal of Biological Macromolecules (2022)
47. Evaluation of the Mechanical Characteristics of Plga and Plga/Fibrin Scaffolds in Providing an Appropriate Environment for Viability and Growth of Human Adipose-Derived Stem Cells, Eurasian Journal of Analytical Chemistry (2018)
48. Evaluation of Structural, Mechanical, and Cellular Behavior of Electrospun Poly-3-Hydroxybutyrate Scaffolds Loaded With Glucosamine Sulfate to Develop Cartilage Tissue Engineering, International Journal of Polymeric Materials and Polymeric Biomaterials (2017)
49. Electrospun Poly(Hydroxybutyrate)/Chitosan Blend Fibrous Scaffolds for Cartilage Tissue Engineering, Journal of Applied Polymer Science (2016)
50. Novel Electrospun Nanofibers of Modified Gelatin-Tyrosine in Cartilage Tissue Engineering, Materials Science and Engineering C (2017)