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Effect of Transforming Growth Factor-Β3 and Bone Morphogenetic Protein-6 Growth Factors on Chondrogenic Differentiation of Adipose-Derived Stem Cells in Alginate Scaffold



Hashemibeni B1 ; Razavi S1 ; Esfandiary E1 ; Karbasi S2 ; Mardani M1 ; Sadeghi F1 ; Esfahani MN3 ; Nadali F4 ; Shafiezade H1
Authors
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Authors Affiliations
  1. 1. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Embryology, Cell Sciences Resaerch Center (ACECR), Royan Institute, Isfahan, Iran
  4. 4. Department of Pathology and Hematology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2010

Abstract

Background: Cartilage damages and diseases such as osteoarthritis are rather worldwide problems of many people. In addition, articular cartilage has a limited ability to repair. Current treatment methods for cartilage tissue injuries lead to formation of fibrous tissue, apoptosis, and further cartilage degeneration. Therefore, engineering of cartilage tissue with adult stem cells is considered necessary. This study aimed to investigate the effect of TGF-β3 and BMP-6 growth factors on chondrogenic differentiation of adiposederived stem cells in alginate scaffold. Methods: Stem cells were isolated from human subcutaneous adipose tissue with collagenase enzyme digestion. The isolated cells were treated with chondrogenic medium supplemented with TGF-β3 and BMP-6 on alginate scaffold then harvested after 3 weeks. Histological, immunohistochemical and RT-PCR methods were performed for assessment of cartilage-specific markers. Findings: Our results revealed that chondrogenesis of ADSCs on alginate scaffold induced by TGF-β3 and BMP-6 growth factors. Immunohistochemical method was shown deposition of typical cartilage extracellular matrix components such as type II collagen and aggrecan in constructs. RT-PCR analysis of cartilage-specific genes, also confirmed the induction of the chondrocytic phenotype upon stimulation with TGF-β3 and BMP-6. Also, TGF-β3 and BMP-6 together increased the expression of aggrecan gene in compare with one growth factor alone. Conclusion: It can be concluded that TGF-β3 and BMP-6 promote chondrogenesis of ADSCs in 3-D culture and the use of both growth factors together increase the expression of aggrecan gene in compare with one growth factor alone.
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