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New Long Noncoding Rna Biomarkers and Cerna Networks on Mir‑616‑3P in Colorectal Cancer: Bioinformatics‑Based Study Publisher



Abdolvand M1, 2, 3 ; Chermahini ZM3 ; Bahaloo S4 ; Emami MH5 ; Fahim A5 ; Rahimi H5 ; Amjadi E5 ; Maghool F5 ; Rohani F6 ; Dadkhah M3 ; Farhadian N3 ; Vatandoust N3 ; Abdolvand S7 ; Darehsari MR7 Show All Authors
Authors
  1. Abdolvand M1, 2, 3
  2. Chermahini ZM3
  3. Bahaloo S4
  4. Emami MH5
  5. Fahim A5
  6. Rahimi H5
  7. Amjadi E5
  8. Maghool F5
  9. Rohani F6
  10. Dadkhah M3
  11. Farhadian N3
  12. Vatandoust N3
  13. Abdolvand S7
  14. Darehsari MR7
  15. Chehelgerdi M7
  16. Beni FA1, 2, 3
  17. Khodadoostan M8
  18. Hemati S9
  19. Salehi M1, 2, 3
Show Affiliations
Authors Affiliations
  1. 1. Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Medical Genetics Research Center of Genome, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Biology, Faculty of Sciences, Yazd University, Yazd, Iran
  5. 5. Poursina Hakim Digestive Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  6. 6. Faculty of Veterinary Medicine of Shahrekord, Shahrekord, Iran
  7. 7. Department of Genetics, Faculty of Sciences, Islamic Azad University, Shahrekord, Iran
  8. 8. Department of Gastroenterology and Hepatology, AlZahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran
  9. 9. Department of Radiooncology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Research in Medical Sciences Published:2024


Abstract

Background: Cancer development is aided by the role of long noncoding RNAs (lncRNAs) that act as competing endogenous RNAs (ceRNAs) absorbing microRNAs (miRNAs). We aimed to discover a novel regulatory axis in colorectal cancer (CRC) and potential biomarkers based on miR‑616‑3p. Materials and Methods: The gene expression omnibus database was mined for differentially expressed lncRNAs (DELs) and mRNAs. LncRNAs and mRNAs were predicted using the RegRNA and TargetScan databases. A combination of the ciBioPortal and Ensemble databases was used to locate the mRNAs. Cytoscape 3.7.1‑built CeRNA networks. A quantitative real‑time polymerase chain reaction (qRT‑PCR) was utilized to confirm the expression levels of these RNA molecules. Statistical analyses were implemented by GraphPad Prism 9. Results: qRT‑PCR showed (Linc01282, lnc‑MYADM‑1:1, and Zinc Finger Protein 347 [ZNF347]) were overexpressed whereas, (salt‑inducible kinases 1 [SIK1], and miR‑616‑3p) were down regulated. Conclusion: These results identify unique, unreported lncRNAs as CRC prognostic biomarkers, as well as prospective mRNAs as new treatment targets and predictive biomarkers for CRC. In addition, our study uncovered unexplored ceRNA networks that should be studied further in CRC. © 2024 Journal of Research in Medical Sciences Published by Wolters Kluwer - Medknow.
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