New Long Noncoding Rna Biomarkers and Cerna Networks on Mir‑616‑3P in Colorectal Cancer: Bioinformatics‑Based Study Publisher

Summary: Study identifies new RNA biomarkers for colorectal cancer, revealing potential treatment targets. #ColorectalCancer #Biomarkers

Abdolvand M^{1, 2, 3} ; Chermahini ZM³ ; Bahaloo S⁴ ; Emami MH⁵ ; Fahim A⁵ ; Rahimi H⁵ ; Amjadi E⁵ ; Maghool F⁵ ; Rohani F⁶ ; Dadkhah M³ ; Farhadian N³ ; Vatandoust N³ ; Abdolvand S⁷ ; Darehsari MR⁷ Show All Authors
Source: Journal of Research in Medical Sciences Published:2024

Abstract

Background: Cancer development is aided by the role of long noncoding RNAs (lncRNAs) that act as competing endogenous RNAs (ceRNAs) absorbing microRNAs (miRNAs). We aimed to discover a novel regulatory axis in colorectal cancer (CRC) and potential biomarkers based on miR‑616‑3p. Materials and Methods: The gene expression omnibus database was mined for differentially expressed lncRNAs (DELs) and mRNAs. LncRNAs and mRNAs were predicted using the RegRNA and TargetScan databases. A combination of the ciBioPortal and Ensemble databases was used to locate the mRNAs. Cytoscape 3.7.1‑built CeRNA networks. A quantitative real‑time polymerase chain reaction (qRT‑PCR) was utilized to confirm the expression levels of these RNA molecules. Statistical analyses were implemented by GraphPad Prism 9. Results: qRT‑PCR showed (Linc01282, lnc‑MYADM‑1:1, and Zinc Finger Protein 347 [ZNF347]) were overexpressed whereas, (salt‑inducible kinases 1 [SIK1], and miR‑616‑3p) were down regulated. Conclusion: These results identify unique, unreported lncRNAs as CRC prognostic biomarkers, as well as prospective mRNAs as new treatment targets and predictive biomarkers for CRC. In addition, our study uncovered unexplored ceRNA networks that should be studied further in CRC. © 2024 Journal of Research in Medical Sciences Published by Wolters Kluwer - Medknow.