Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
In Silico Identification and in Vitro Evaluation of Mrps30-Dt Lncrna and Mrps30 Gene Expression in Breast Cancer Publisher Pubmed



Shirani N1 ; Mahdiesferizi R1, 2 ; Eshraghi Samani R3 ; Tahmasebian S2 ; Yaghoobi H1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
  2. 2. Department of Medical Biotechnology, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran
  3. 3. Department of General Surgery, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Cancer Reports Published:2024


Abstract

Background: It has been reported that long non-coding RNAs (lncRNAs) can play important roles in a variety of biological processes and cancer regulatory networks, including breast cancer. Aims: This study aimed to identify a novel upregulated lncRNA in breast cancer and its associated gene using bioinformatics analysis, and then evaluate their potential roles in breast cancer. Methods and Results: Extensive in silico studies were performed using various bioinformatics databases and tools to identify a potential upregulated breast cancer-associated lncRNA and its co-expressed gene, and to predict their potential roles, functions, and interactions. The expression level of MRPS30-DT lncRNA and MRPS30 was assessed in both BC tissues and cell lines using qRT-PCR technology. MRPS30-DT lncRNA and MRPS30 were selected as target genes using bioinformatics analysis. We found that MRPS30-DT and MRPS30 were significantly overexpressed in BC tissues compared with normal tissues. Also, MRPS30 showed upregulation in all three BC cell lines compared with HDF. On the other hand, MRPS30-DT significantly increased in MDA-MB-231 compared with HDF. While the expression of MRPS30-DT was significantly dropped in the resistance cell line MCF/MX compared to HDF and MCF7. Moreover, bioinformatics analysis suggested that MRPS30-DT and MRPS30 may play a potential role in BC through their involvement in some cancer signaling pathways and processes, as well as through their interaction with TFs, genes, miRNAs, and proteins related to carcinogenesis. Conclusions: Overall, our findings showed the dysregulation of MRPS30-DT lncRNA and MRPS30 may provide clues for exploring new therapeutic targets or molecular biomarkers in BC. © 2024 The Author(s). Cancer Reports published by Wiley Periodicals LLC.
Experts (# of related papers)
Other Related Docs
13. H19: A Regulatory Biomarker With Prognostic Value in Leukemias, Eurasian Journal of Medicine and Oncology (2019)
22. Long Noncoding Rnas in Gastric Cancer Carcinogenesis and Metastasis, Briefings in Functional Genomics (2017)
23. Mir-370 Expression Analysis in Breast Cancer, Journal of Isfahan Medical School (2015)
24. Opposite Trends of Gas6 and Gas6-As Expressions in Breast Cancer Tissues, Experimental and Molecular Pathology (2021)
27. Exploring Conserved Mrna-Mirna Interactions in Colon and Lung Cancers, Gastroenterology and Hepatology from Bed to Bench (2017)
29. Downregulation of Mir100hg Induces Apoptosis in Human Megakaryoblastic Leukemia Cells, Indian Journal of Hematology and Blood Transfusion (2021)
42. Long Non-Coding Rnas As Potential Biomarkers or Therapeutic Targets in Gastric Cancer, Gastroenterology and Hepatology from Bed to Bench (2023)
44. Long Non-Coding Rnas and Melanoma: From Diagnosis to Therapy, Pathology Research and Practice (2023)
49. Long Non-Coding Rnas Involved in Retinoblastoma, Journal of Cancer Research and Clinical Oncology (2023)