Association Between the Rs7903146 (C/T) Polymorphism of the Tcf7l2 and Serum Concentration of Copper in Type 2 Diabetes Patients



Alian F¹ ; Nabiafjadi M¹ ; Habibollahi S² ; Negahdary M^{3, 4} ; Palizban A⁵ Show Affiliations
Source: Malaysian Journal of Biochemistry and Molecular Biology Published:2019

Abstract

Type 2 diabetes (T2D) is a multifactorial illness, while genetic and environmental factors are involved in the progression of the disease. The aim of this study was to evaluate the serum level of copper in diabetic and healthy patients and the association of it with rs7903146 (C/T) polymorphism of the TCF7L2. In this study, 132 samples were considered and evaluated. The DNA was extracted from all whole blood samples. Subsequently, genotyping was performed. Then, genotype and allele frequencies were evaluated in both healthy and patient groups. In addition, the serum copper level was measured. Data analysis was performed using SPSS software. The found data showed that the mean of the serum copper level was significantly increased, in diabetic patients with TT genotype (385.56 ±30.38), in comparison with healthy subjects (135.37 ±19.18) (p=0). Moreover, a significant increase in the mean of serum copper level in diabetic patients with genotype CT (387.19 ±34.94), compared to healthy subjects (124.73 ±10.31) (p=0), as well as a significant increase in the mean of serum copper level in diabetic individuals with CC genotype (429.29 ±44.36), compared to healthy subjects (141.78 ±12.35) were observed (p=0). Finally results showed that the SNP, rs7903146 is a risk factor for T2D, in specific T allele in both homozygous and heterozygous states. Findings of this study also showed that the serum copper level in diabetic patients was higher than the healthy ones. However, there was no significant difference in the serum copper level in diabetic patients with CC and TT genotype. © 2019 Malaysian Society for Biochemistry and Molecular Biology. All rights reserved.