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Association of Polymorphism of Ser311cys Paraoxonase-2 Gene With Type 2 Diabetes Mellitus in Iran



Andalib S1 ; Vaseghi G2 ; Motavallian A3 ; Sadeghi HM4 ; Eshraghi A2 ; Amini M5 ; Majlesi AR6
Authors
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Authors Affiliations
  1. 1. Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  2. 2. Physiology Reseach Center, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Pharmacology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
  4. 4. Department of Pharmaceutical Biotechnology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  5. 5. Sedigheh Tahereh Research Complex, Isfahan University of Medical Sciences, Isfahan, Iran
  6. 6. Department of Genetics, Tehran Medical Branch, Islamic Azad University, Tehran, Iran

Source: International Journal of Preventive Medicine Published:2013

Abstract

Background: It is believed that paraoxonase-2 gene polymorphism is associated with type 2 diabetes. This study is aimed to investigate the association between paraoxonase-2 gene polymorphism and type 2 diabetes in an Iranian population. Methods: This study was performed on 200 individuals including 100 diabetics and 100 healthy subjects. Genomic DNA was extracted from peripheral blood leukocytes, and PCR-RFLP was carried out. Palindromic sequence in PON2 gene was recognized by Dde1 restriction endonuclease. In order to visualize restriction products, electrophoresis was carried out using polyacrylamide gel (8%) and ethidium bromide staining. Results: The expected PCR product of 331 bp was obtained. Digestion of this product with DdeI showed four Ser homozygotes, three Cys homozygotes, and five Ser311 Cys heterozygotes. The gene frequency of Cys (C) in diabetic subjects was significantly higher than in healthy subjects. Conclusions: This study suggests that an association exists between Ser311 Cys polymorphism and type 2 diabetes mellitus.
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