The Effect of Salusin-Β on Expression of Pro- and Anti-Inflammatory Cytokines in Human Umbilical Vein Endothelial Cells (Huvecs)

The Effect of Salusin-Β on Expression of Pro- and Anti-Inflammatory Cytokines in Human Umbilical Vein Endothelial Cells (Huvecs) Publisher

Esfahani M¹ ; Saidijam M² ; Najafi R² ; Goodarzi MT³ ; Movahedian A¹

Show Affiliations

1. Isfahan Pharmaceutical Sciences Research Center, Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
2. Research Center for Molecular Medicine, Hamedan University of Medical Sciences, Hamedan, Iran
3. Research Center for Molecular Medicine, Department of Clinical Biochemistry, School of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran

Source: ARYA Atherosclerosis Published:2018

Abstract

BACKGROUND: Atherosclerosis is one of the predominant causes of cardiovascular disease (CVD). Several studies indicated the significant pathophysiological role of salusin-β in atherosclerosis. Cytokines are involved in all stages of atherosclerosis. Therefore, we aimed to assess the effect of salusin-β on interleukin 6 (IL-6), interleukin 8 (IL-8), interleukin 18 (IL-18) (as inflammatory cytokines) and interleukin 1Ra (IL-1Ra) (as anti-inflammatory cytokines) levels in human umbilical vein endothelial cells (HUVECs). METHODS: The HUVECs were cultured in HUVEC completed medium and treated with different doses of salusin-β for 6 and 12 hours. For the investigation of nuclear factor kβ (NF-kβ) signaling pathway involvement, cells were treated in the presence or absence of Bay 11-7082 (as NF-kβ inhibitor). The mRNA expression and protein level of cytokines were measured by a real-time polymerase chain reaction (PCR) system and enzyme-linked immunosorbent assay (ELISA) method, respectively. RESULTS: Salusin-β increased mRNA expression and protein level of IL-6, IL-8 and IL-18. This protein decreased mRNA and protein level of IL-1Ra in HUVECs. NF-kβ signaling pathway was involved in the up-regulatory effect of salusin-β on mRNA expression of pro-inflammatory cytokines. The down-regulatory effect of salusin-β on IL-1Ra expression could not be influenced by Bay 11-7082 pre-treatment. CONCLUSION: It seems that salusin-β may participate in a cascade pathway in vascular inflammation. Our findings suggested that salusin-β has potential use as a therapeutic target for atherosclerosis. © 2018, Isfahan University of Medical Sciences(IUMS). All rights reserved.

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Style	Citing Format
MLA	Esfahani M, et al.. "The Effect of Salusin-Β on Expression of Pro- and Anti-Inflammatory Cytokines in Human Umbilical Vein Endothelial Cells (Huvecs)." ARYA Atherosclerosis, vol. 14, no. 1, 2018, pp. 1-10.
APA	Esfahani M, Saidijam M, Najafi R, Goodarzi MT, Movahedian A (2018). The Effect of Salusin-Β on Expression of Pro- and Anti-Inflammatory Cytokines in Human Umbilical Vein Endothelial Cells (Huvecs). ARYA Atherosclerosis, 14(1), 1-10.
Chicago	Esfahani M, Saidijam M, Najafi R, Goodarzi MT, Movahedian A. "The Effect of Salusin-Β on Expression of Pro- and Anti-Inflammatory Cytokines in Human Umbilical Vein Endothelial Cells (Huvecs)." ARYA Atherosclerosis 14, no. 1 (2018): 1-10.
Harvard	Esfahani M et al. (2018) 'The Effect of Salusin-Β on Expression of Pro- and Anti-Inflammatory Cytokines in Human Umbilical Vein Endothelial Cells (Huvecs)', ARYA Atherosclerosis, 14(1), pp. 1-10.
Vancouver	Esfahani M, Saidijam M, Najafi R, Goodarzi MT, Movahedian A. The Effect of Salusin-Β on Expression of Pro- and Anti-Inflammatory Cytokines in Human Umbilical Vein Endothelial Cells (Huvecs). ARYA Atherosclerosis. 2018;14(1):1-10.
BibTex	@article{ author = {Esfahani M and Saidijam M and Najafi R and Goodarzi MT and Movahedian A}, title = {The Effect of Salusin-Β on Expression of Pro- and Anti-Inflammatory Cytokines in Human Umbilical Vein Endothelial Cells (Huvecs)}, journal = {ARYA Atherosclerosis}, volume = {14}, number = {1}, pages = {1-10}, year = {2018} }
RIS	TY - JOUR AU - Esfahani M AU - Saidijam M AU - Najafi R AU - Goodarzi MT AU - Movahedian A TI - The Effect of Salusin-Β on Expression of Pro- and Anti-Inflammatory Cytokines in Human Umbilical Vein Endothelial Cells (Huvecs) JO - ARYA Atherosclerosis VL - 14 IS - 1 SP - 1 EP - 10 PY - 2018 ER -

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