Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Detection of the Effect of Bee Propolis Ethanol Extract Against Molecularly Identified Multiple-Drug Resistant Bacterial Isolates in Wounds of Hospitalized Patients in Ahvaz City, Iran Publisher



Eidisheikhrobat A1 ; Naghavi NS1 ; Karbasizadeh V2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
  2. 2. Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2018


Abstract

Background: Multiple-drug resistance (MDR) is one of the medical challenges in medicine. The aim of present study was molecular identification of multiple-drug resistant bacterial isolates in wounds of patients in Ahvaz City, Iran, hospitals, and detection of the effect of bee propolis ethanol extract on these bacteria. Methods: 120 samples was obtained from bed, burn, and diabetic foot wounds of patients hospitalized in 5 hospitals in Ahwaz City from May to July 2016. Multiple-drug resistant isolates were selected using disk diffusion method and were identified via sequencing 16SrRNA gene. Propolis ethanol extract was prepared using soaking method and its effect as an antibacterial agent was tested on the isolates by well diffusion and microdilution methods. Findings: 6 multiple-drug resistant isolates including Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, Enterococcus faecium, and Enterococcus faecalis were identified among 25 antibiotic-resistant isolates from different wounds. The concentration of 25 mg/ml of propolis ethanol extract was effective on Gram-positive bacteria in well diffusion method. In microdilution method, the most sensitivity was seen in Staphylococcus aureus with minimum inhibitory concentration (MIC) of 25 mg/ml and minimum bactericidal concentration (MBC) of 50 mg/ml, and then in Enterococcus faecium, and Enterococcus faecalis minimum inhibitory concentration of 100 mg/ml and minimum bactericidal concentration of 200 mg/ml. Gram-negative bacteria were resistant to all concentrations of extract in both methods. Conclusion: The isolated multiple-drug resistant Gram-positive bacteria which were from problematic agents in treatment of wounds, showed high sensitivity to propolis ethanolic extract. © 2018, Isfahan University of Medical Sciences(IUMS). All rights reserved.
Related Docs
View other Related Docs
1. Evaluation of Pseudomonas Aeroginosa Isolated From Cutaneous Infections and Determination of Drug Resistance Pattern in Patients of Alzahra Hospital in Esfahan, Journal of Isfahan Medical School (2010)
2. Resistance to Colistin and Detection of Mcr-1 Gene in Multidrug-Resistant Pseudomonas Aeruginosa Isolated From Wounds of the Hospitalized Burned Patients, Journal of Isfahan Medical School (2022)
3. Prevalence of Blaveb and Blatem Genes, Antimicrobial Resistance Pattern and Biofilm Formation in Clinical Isolates of Pseudomonas Aeruginosa From Burn Patients in Isfahan, Iran, Gene Reports (2021)
4. Frequency and Resistance Patterns of Bacterial Isolates From Burn Wounds Infections in Isfahan, Iran, Journal of Isfahan Medical School (2013)
5. Investigation of Integron Classes 1, 2, and 3 Among Multi-Drug Resistant Staphylococcus Aureus Isolates in Iran: A Multi-Center Study, BMC Infectious Diseases (2024)
6. Detection of Genes Involved in Biofilm Formation in Mdr and Xdr Acinetobacter Baumannii Isolated From Human Clinical Specimens in Isfahan, Iran, Archives of Clinical Infectious Diseases (2019)
7. The Frequency of Methicillin-Resistant Staphylococcus Aureus Strains Isolated From Patients of a Teaching Hospital in Isfahan City, Iran, Journal of Isfahan Medical School (2021)
8. Identification of Beta-Lactamase-Producing Pseudomonas Aeruginosa With Multiple Antibiotic Resistances, Journal of Isfahan Medical School (2011)
Experts (# of related papers)
View all Related Experts
Hossein Fazeli (14)
Bahram Nasr Esfahani (11)
Other Related Docs
9. Antibacterial Activity of Tedizolid, a Novel Oxazolidinone Against Methicillin-Resistant Staphylococcus Aureus: A Systematic Review and Meta-Analysis, Microbial Drug Resistance (2019)
10. Bacterial Profile and Antimicrobial Resistance Patterns of Infected Diabetic Foot Ulcers in Iran: A Systematic Review and Meta-Analysis of Cross-Sectional Studies, International Journal of Lower Extremity Wounds (2022)
11. Evaluation of Chlorogenic Acid and Carnosol for Anti-Efflux Pump and Anti-Biofilm Activities Against Extensively Drug-Resistant Strains of Staphylococcus Aureus and Pseudomonas Aeruginosa, Microbiology Spectrum (2024)
12. Molecular Detection of Type Ii Toxin-Antitoxin Systems and Their Association With Antibiotic Resistance and Biofilm Formation in Clinical Acinetobacter Baumannii Isolates of Burn Patients, Journal of Mazandaran University of Medical Sciences (2021)
13. Evaluation of Wound Healing and Antimicrobial Properties of Hydrogel Dressings of Starch Containing Ethanolic Extract of Propolis in the Rat, Journal of Isfahan Medical School (2018)
14. Identification of Genomic Species Ofacinetobacter Isolated From Burns of Icu Patients, Archives of Iranian Medicine (2015)
15. Molecular Epidemiology of Colistin-Resistant Pseudomonas Aeruginosa Producing Ndm-1 From Hospitalized Patients in Iran, Iranian Journal of Basic Medical Sciences (2019)
16. Molecular Characterization and Phage Typing of Methicillin-Resistant Staphylococcus Aureus Isolated From Clinical Samples in Isfahan, Iran, Jundishapur Journal of Microbiology (2023)
17. Identification of Nora, Qacab and Smr Genes and Determination of Antibiotic and Biocide Resistance Patterns in Methicillin-Resistant Staphylococcus Aureus Isolated From Isfahan-Iran, Reviews and Research in Medical Microbiology (2024)
18. Clonal Repetitive Element Polymerase Chain Reaction Patterns of Pseudomonas Aeruginosa in Diabetic Foot Ulcers, Iran, IJID Regions (2025)
19. Investigating of Four Main Carbapenem-Resistance Mechanisms in High-Level Carbapenem Resistant Pseudomonas Aeruginosa Isolated From Burn Patients, Journal of the Chinese Medical Association (2018)
20. First Prevalence of Metallo Beta-Lactamases Producing Enterobacteriacea in Iranian Cancer Patients, Annali di Igiene Medicina Preventiva e di Comunita (2019)
21. Identification of Blaoxa−51−23−58, Blavim, Blandm, and Blaimp Carbapenemase Genes in Acinetobacter Baumannii Isolates From Hospitalized Patients, BMC Research Notes (2024)
22. Inducible Clindamycin Resistance in Clinical Isolates of Staphylococcus Aureus Due to Erm Genes, Iran, Iranian Journal of Microbiology (2014)
23. The Study of Antibacterial Effect of Humulus Lupulus on Some of Gram Positive & Gram Negative Bacteria, Journal of Medicinal Plants (2009)
24. High Prevalence of Multidrug Resistance and Metallo-Beta-Lactamase (Mβl) Producing Acinetobacter Baumanniiisolated From Patients in Icu Wards, Hamadan, Iran, Journal of Research in Health Sciences (2013)
25. From the Urinary Catheter to the Prevalence of Three Classes of Integrons, Β -Lactamase Genes, and Differences in Antimicrobial Susceptibility of Proteus Mirabilis and Clonal Relatedness With Rep-Pcr, BioMed Research International (2021)
26. Clonal Relatedness Among Imipenem-Resistant Pseudomonas Aeruginosa Isolated From Icu-Hospitalized Patients, Critical Care Research and Practice (2015)
27. The Emerging Role of Probiotics and Their Derivatives Against Biofilm-Producing Mrsa: A Scoping Review, Genetics Research (2022)
28. Antibacterial Effect of Allium Ampeloprasum and Allium Porrum Extracts on Staphylococcus Aureus and Pseudomonas Aeruginosa, Journal of Pharmacopuncture (2023)
29. Phenotypic and Genotypic Characterization of Macrolide Resistance Among Staphylococcus Aureus Isolates in Isfahan, Iran, Iranian Journal of Microbiology (2017)
30. Prevalence of Blatem and Blaveb Genes in Multidrug-Resistant Acinetobacter Baumannii Strains Isolated From Burn Wound Infection in Isfahan, Iran, Infection, Epidemiology and Microbiology (2020)
31. The Survey of Fungal Wounds Infections in Burn Patients in Isfahan, Iran, Journal of Isfahan Medical School (2017)
32. Determination of Antibiotic Resistance Pattern and Prevalence of Oxa-Type Carbapenemases Among Acinetobacter Baumannii Clinical Isolates From Inpatients in Isfahan, Central Iran, Infezioni in Medicina (2018)
33. Characterization of Clonal Groups of Antibiotic-Resistant Biofilm-Forming Staphylococcus Aureus Strains Isolated From Patients With Urinary Tract Infections in Isfahan, Iran, Archives of Clinical Infectious Diseases (2024)
34. Resistance to Antibiotics and Biocides and Prevalence of Qacab, Smr and Nora Genes in Vancomycin-Resistant Enterococci Isolated From Patients of Medical Education Centers in Isfahan, Iran, Journal of Isfahan Medical School (2023)
35. Antimicrobial Susceptibility Pattern of Microorganisms Involved in the Pathogenesis of Surgical Site Infection (Ssi); a 1 Year of Surveillance, Pakistan Journal of Biological Sciences (2008)
36. The Determination of Metallo-Beta-Lactamase Enzymes Prevalence in Pseudomonas Aeruginosa Using Etest and Their Antibiogram Patterns in Kermanshah, Iran, Journal of Kerman University of Medical Sciences (2015)
37. In Vitro Antifungal Activity of Iranian Propolis Against Microsporum Canis, M. Gypseum and M. Nanum, Journal of Biologically Active Products from Nature (2012)
38. Agr Typing of Staphylococcus Aureus Species Isolated From Clinical Samples in Training Hospitals of Isfahan and Shahrekord, BMC Research Notes (2019)
39. Plasmid Mediated Antibiotic Resistance in Isolated Bacteria From Burned Patients, Jundishapur Journal of Microbiology (2015)
40. Cornstarch-Based Wound Dressing Incorporated With Hyaluronic Acid and Propolis: In Vitro and in Vivo Studies, Carbohydrate Polymers (2019)
41. A Propolis Enriched Polyurethane-Hyaluronic Acid Nanofibrous Wound Dressing With Remarkable Antibacterial and Wound Healing Activities, International Journal of Biological Macromolecules (2020)
42. Bacterial Infections and Antimicrobial Resistance Patterns of Burn Wound Infections: A One Year Study From Burn Hospital, Isfahan, Iran, Journal of Advances in Medical and Biomedical Research (2020)
43. Physicochemical, Antimicrobial and Cytotoxic Characteristics of Corn Starch Film Containing Propolis for Wound Dressing, Journal of Polymers and the Environment (2018)
44. Evaluation of the Synergistic Effect of Tomatidine With Several Antibiotics Against Standard and Clinical Isolates of Staphylococcus Aureus, Enterococcus Faecalis, Pseudomonas Aeruginosa and Escherichia Coli, Iranian Journal of Pharmaceutical Research (2017)
45. Antimicrobial Effect of Curcumin Nanoparticles and Ferulago Angulate Boiss Extract Against Methicillin-Resistant Staphylococcus Aureus (Mrsa) Isolated From Wound Infections, BioNanoScience (2024)
46. Emergence of Cefepime Resistance in Gram-Negative Induced Nosocomial Infections, Iranian Journal of Clinical Infectious Diseases (2009)
47. Determination of Biofilm Formation Ability and Antibiotic Resistance of Acinetobacter Baumannii Strains Isolated From Patients With Burn Wound Infection, Journal of Isfahan Medical School (2017)
48. The Effect of Isolated Bacteriophage on Multi-Drug Resistant (Mdr) Pseudomonas Aeruginosa, Journal of Isfahan Medical School (2014)
49. High Prevalence of Multidrug-Resistance Uropathogenic Escherichia Coli Strains, Isfahan, Iran, Journal of Natural Science, Biology and Medicine (2016)
50. Evaluation of the Effect of Smoke of Anbar Nasara (Donkey Dung) Extract on Staphilococcus Aureus and Bacilus Subtilis, Journal of Isfahan Medical School (2017)