Identification of Beta-Lactamase-Producing Pseudomonas Aeruginosa With Multiple Antibiotic Resistances



Fazzeli H¹ ; Faghri J¹ ; Kabiri P² ; Fatahibafghi M¹ ; Arabestani MR³ Show Affiliations
Source: Journal of Isfahan Medical School Published:2011

Abstract

Background: The clinical use of antibiotics in treatment of Pseudomonas aeruginosa infections causes resistant to multiple drugs increasingly. P. aeruginosa could produce beta lactamase enzyme to beta lactam antibiotics that causes many problems. The purpose of this study is analysis of the isolated of Pseudomonas from clinical specimens and this was done for the first time in Isfahan. Methods: A total of 98 isolates of P. aeruginosa from various clinical samples collected, then identified by biochemical tests. The antibiotic sensitivity of strains was performed by Kirby-Bauer method. Findings: The pattern of resistance to antibiotics showed that the greatest strength was in the burn specimen and 30 samples of the strains isolated from burns. In the 30 strains (%100) were resistances to more than three antibiotic (multi-drug resistant). Maximum resistance to Cefodoxime, amoxiclave, Cefexime, Cefotaxime and ceftizoxime respectively and the most sensitive to Ciprofloxsazine and gentamicin. In this study 63 strains showed the full resistance to ceftazidime and Cefotaxime s and ESBL test was done that revealed 23 strains of ESBL were negative and 40 strains did not inhibit by Clavulanic acid. All of the burn samples resistant to ceftazidime and did not inhibit by Clavulanic acid. Conclusion: In regarding to the high prevalence of P. aeruginosa with Multiple Drug Resistant, increased resistance to antibiotics and the high incidence of ESBL in the strains in clinical samples, it is essential, extended control measures to reduce this pathogens. © 2011, Isfahan University of Medical Sciences(IUMS). All rights reserved.