Transplantation of Adult Monkey Neural Stem Cells Into a Contusion Spinal Cord Injury Model in Rhesus Macaque Monkeys

Transplantation of Adult Monkey Neural Stem Cells Into a Contusion Spinal Cord Injury Model in Rhesus Macaque Monkeys

Nemati S¹ ; Jabbari R² ; Hajinasrollah M¹ ; Mehrjerdi NZ¹ ; Azizi H¹ ; Hemmesi K¹ ; Moghiminasr R¹ ; Azhdari Z¹ ; Talebi A³ ; Mohitmafi S⁴ ; Dizaj AVT⁵ ; Sharifi G² ; Baharvand H^{1, 6} ; Rezaee O² Show All Authors

Nemati S¹
Jabbari R²
Hajinasrollah M¹
Mehrjerdi NZ¹
Azizi H¹
Hemmesi K¹
Moghiminasr R¹
Azhdari Z¹
Talebi A³
Mohitmafi S⁴
Dizaj AVT⁵
Sharifi G²
Baharvand H^{1, 6}
Rezaee O²
Kiani S¹

Show Affiliations

1. Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, P.O. Box: 16635-148, Iran
2. Department of Neurosurgical Science, Loghman Hospital, Shahid Behshti University of Medial Sciences, Tehran, Iran
3. Al-Zahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran
4. Department of Clinical Science, Faculty of Veterinary Medicine, Islamic Azad University, Karaj, Iran
5. Department of Reproductive Imaging at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
6. Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran

Source: Cell Journal Published:2014

Abstract

Objective: Currently, cellular transplantation for spinal cord injuries (SCI) is the subject of numerous preclinical studies. Among the many cell types in the adult brain, there is a unique subpopulation of neural stem cells (NSC) that can self-renew and differentiate into neurons. The study aims, therefore, to explore the efficacy of adult monkey NSC (mNSC) in a primate SCI model. Materials and Methods: In this experimental study, isolated mNSCs were analyzed by flow cytometry, immunocytochemistry, and RT-PCR. Next, BrdU-labeled cells were transplanted into a SCI model. The SCI animal model was confirmed by magnetic resonance imaging (MRI) and histological analysis. Animals were clinically observed for 6 months. Results: Analysis confirmed homing of mNSCs into the injury site. Transplanted cells expressed neuronal markers (TubIII). Hind limb performance improved in transplanted animals based on Tarlov's scale and our established behavioral tests for monkeys. Conclusion: Our findings have indicated that mNSCs can facilitate recovery in contusion SCI models in rhesus macaque monkeys. Additional studies are necessary to determine the improvement mechanisms after cell transplantation.

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Style	Citing Format
MLA	Nemati S, et al.. "Transplantation of Adult Monkey Neural Stem Cells Into a Contusion Spinal Cord Injury Model in Rhesus Macaque Monkeys." Cell Journal, vol. 16, no. 2, 2014, pp. 117-130.
APA	Nemati S, Jabbari R, Hajinasrollah M, Mehrjerdi NZ, Azizi H, Hemmesi K, Moghiminasr R, Azhdari Z, Talebi A, Mohitmafi S, Dizaj AVT, Sharifi G, Baharvand H, Rezaee O, Kiani S (2014). Transplantation of Adult Monkey Neural Stem Cells Into a Contusion Spinal Cord Injury Model in Rhesus Macaque Monkeys. Cell Journal, 16(2), 117-130.
Chicago	Nemati S, Jabbari R, Hajinasrollah M, Mehrjerdi NZ, Azizi H, Hemmesi K, Moghiminasr R, et al.. "Transplantation of Adult Monkey Neural Stem Cells Into a Contusion Spinal Cord Injury Model in Rhesus Macaque Monkeys." Cell Journal 16, no. 2 (2014): 117-130.
Harvard	Nemati S et al. (2014) 'Transplantation of Adult Monkey Neural Stem Cells Into a Contusion Spinal Cord Injury Model in Rhesus Macaque Monkeys', Cell Journal, 16(2), pp. 117-130.
Vancouver	Nemati S, Jabbari R, Hajinasrollah M, Mehrjerdi NZ, Azizi H, Hemmesi K, et al.. Transplantation of Adult Monkey Neural Stem Cells Into a Contusion Spinal Cord Injury Model in Rhesus Macaque Monkeys. Cell Journal. 2014;16(2):117-130.
BibTex	@article{ author = {Nemati S and Jabbari R and Hajinasrollah M and Mehrjerdi NZ and Azizi H and Hemmesi K and Moghiminasr R and Azhdari Z and Talebi A and Mohitmafi S and Dizaj AVT and Sharifi G and Baharvand H and Rezaee O and Kiani S}, title = {Transplantation of Adult Monkey Neural Stem Cells Into a Contusion Spinal Cord Injury Model in Rhesus Macaque Monkeys}, journal = {Cell Journal}, volume = {16}, number = {2}, pages = {117-130}, year = {2014} }
RIS	TY - JOUR AU - Nemati S AU - Jabbari R AU - Hajinasrollah M AU - Mehrjerdi NZ AU - Azizi H AU - Hemmesi K AU - Moghiminasr R AU - Azhdari Z AU - Talebi A AU - Mohitmafi S AU - Dizaj AVT AU - Sharifi G AU - Baharvand H AU - Rezaee O AU - Kiani S TI - Transplantation of Adult Monkey Neural Stem Cells Into a Contusion Spinal Cord Injury Model in Rhesus Macaque Monkeys JO - Cell Journal VL - 16 IS - 2 SP - 117 EP - 130 PY - 2014 ER -

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