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A Biomimetic Injectable Chitosan/Alginate Hydrogel Biocopmosites Encapsulating Selenium- Folic Acid Nanoparticles for Regeneration of Spinal Cord Injury: An in Vitro Study Publisher Pubmed



Farzan M1, 2 ; Soleimannejad M3, 4 ; Shariat S5, 6 ; Heidari Sureshjani M3 ; Gholipour A7 ; Ashrafi Dehkordi K3, 8 ; Alerasoul Dehkordi SMR2 ; Farzan M1, 2
Authors
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Authors Affiliations
  1. 1. Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran
  2. 2. Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
  3. 3. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
  4. 4. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran
  5. 5. Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
  6. 6. Department of Biomaterials and Tissue Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  7. 7. Department of Microbiology and Immunology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
  8. 8. Department of Molecular Medicine, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran

Source: International Journal of Biological Macromolecules Published:2025


Abstract

Spinal cord injury (SCI) poses significant challenges to regenerative medicine due to its limited self-repair capabilities. In this study, we engineered a biomimetic injectable hydrogel using modified chitosan and alginate biopolymers encapsulating selenium-folic acid nanoparticles (Se-FA NPs) to facilitate SCI regeneration. The hydrogel exhibited a unique porous structure attributed to the incorporation of nanofiber fragments, enhancing its biocompatibility and bioactivity. Through a series of in vitro evaluations, including cell viability assays, proliferation studies, gene expression analysis, we assessed the hydrogel's cytocompatibility and its potential for supporting neural cell growth. Our results demonstrate the promising efficacy of the hydrogel in providing a conducive microenvironment for neural tissue regeneration. Moreover, the sustained release of Se-FA NPs from the hydrogel system offers neuroprotective, antioxidative, and anti-inflammatory benefits crucial for SCI therapy. Overall, our biomimetic hydrogel biocomposites hold great potential as a therapeutic strategy for promoting spinal cord regeneration, highlighting their significance in advancing the field of regenerative medicine. © 2024
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