Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Effect of Estradiol on Production of Type Ii Collagen and Aggrecan in Chondrogenic Proccess



Hashemibeni B1, 2 ; Sadeghi F1, 2 ; Ansar M3 ; Kabiri A3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Anatomical Sciences, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran

Source: Journal of Isfahan Medical School Published:2014

Abstract

Background: Sex hormones play important role in proliferation, differentiation, maturation and the scheduled death of chondrocytes. Although, some studies report the regulatory role of estrogen in the development and progression of cartilage, some of the mechanisms still remain unclear, including the role of estrogen in the expression of cartilage-specific genes in chondrogenesis process. We studied this role in the present study. Methods: We used adipose-derived stem cells, which were previously differentiated into cartilage tissue in pellet culture system in the control (without estrogen in culture medium) and experimental (with estrogen in culture medium) groups. Production of chondrogenesis markers, type II collagen and aggrecan were evaluated in experimental and control groups via immunohistochemical (IHC) technique. Then, the results were evaluated with Image-J software and statistical analysis were done. Findings: Estrogen led to decrease of type II collagen and increase of aggrecan production. Conclusion: This study showed that chondrogenesis could be affected by estrogen. © 2014, Isfahan University of Medical Sciences(IUMS). All rights reserved.
Related Docs
View other Related Docs
1. The Effect of Bmp-6 Growth Factor on Differentiation of Adipose-Derived Stem Cells Into Chondrocyte in Pellet Culture System, Journal of Isfahan Medical School (2009)
2. Effect of Transforming Growth Factor-Β3 and Bone Morphogenetic Protein-6 Growth Factors on Chondrogenic Differentiation of Adipose-Derived Stem Cells in Alginate Scaffold, Journal of Isfahan Medical School (2010)
3. Comparison Between the Effect of Kartogenin and Tgfβ3 on Chondrogenesis of Human Adipose- Derived Stem Cells in Fibrin Scaffold, Bratislava Medical Journal (2017)
4. Effects of Fgf-2 on Human Adipose Tissue Derived Adult Stem Cells Morphology and Chondrogenesis Enhancement in Transwell Culture, Biochemical and Biophysical Research Communications (2012)
5. Comparison of Cd44 and Cd14 Surface Markers in Adipose-Derived Stem Cells and Differentiated Chondrocytes During Chondrogenesis, Journal of Isfahan Medical School (2014)
6. Impact of Fibrin on the Chondrogenic Avocado Soybean Unsaponifiables on Poly (Lactic-Co-Glycolic) Acid Scaffold, Biointerface Research in Applied Chemistry (2021)
7. The Effect of Platelet Rich Plasma on Chondrogenic Differentiation of Human Adipose Derived Stem Cells in Transwell Culture, Iranian Journal of Basic Medical Sciences (2013)
8. Comparison Between Chondrogenic Markers of Differentiated Chondrocytes From Adipose Derived Stem Cells and Articular Chondrocytes in Vitro, Iranian Journal of Basic Medical Sciences (2013)
Experts (# of related papers)
View all Related Experts
Batool Hashemibeni (32)
Ebrahim Esfandiari (17)
Other Related Docs
9. Effects of Avocado/Soybean on the Chondrogenesis of Human Adipose-Derived Stem Cells Cultured on Polylactic-Co-Glycolic Acid/Fibrin Hybrid Scaffold, Journal of Applied Biotechnology Reports (2019)
10. Cartilage Tissue Formation From Human Adipose-Derived Stem Cells Via Herbal Component (Avocado/Soybean Unsaponifiables) in Scaffold-Free Culture System, Dental Research Journal (2020)
11. Investigation and Comparison of the Effect of Tgf-Β3, Kartogenin and Avocado/Soybean Unsaponifiables on the In-Vitro and In-Vivo Chondrogenesis of Human Adipose-Derived Stem Cells on Fibrin Scaffold, Iranian Journal of Pharmaceutical Research (2021)
12. The Effect of Pomegranate Extract on Producing Type Ii Collagen in Differentiation of Adipose-Derived Stem Cells Into Chondrocytes, Journal of Isfahan Medical School (2018)
13. Evaluation of Pomegranate Seed Extract and Tgf-Β3 on Chondrogenic Differentiation of Human Adipose-Derived Stem Cells, Journal of Applied Biotechnology Reports (2022)
14. Comparison of the Efficacy of Piascledine and Transforming Growth Factor Β1 on Chondrogenic Differentiation of Human Adipose-Derived Stem Cells in Fibrin and Fibrin-Alginate Scaffolds, Iranian Journal of Basic Medical Sciences (2018)
15. Comparison of Poly(Lactic-Co-Glycolic Acid) and Poly(Lactic-Co-Glycolic Acid)/Hyaluronic Acid Based Scaffolds for Chondrogenesis of Human Adipose-Derived Stem Cells by Avocado/Soybean Unsaponifiables, Journal of Kerman University of Medical Sciences (2021)
16. Chondrogenic Differentiation of Human Scalp Adipose-Derived Stem Cells in Polycaprolactone Scaffold and Using Freeze Thaw Freeze Method, Journal of Cellular Physiology (2018)
17. Comparison of Fibrin and Plga/Fibrin Scaffolds for Chondrogenesis of Human Adipose Derived Stem Cells by Icariin, Journal of Kerman University of Medical Sciences (2020)
18. Effect of Piasclidin on Induction of Chondrogenesis by Human Adipose-Derived Stem Cells in Fibrin Scaffold, Journal of Isfahan Medical School (2016)
19. Induction of Chondrogenic Differentiation of Human Adipose-Derived Stem Cells With Tgf-Β3 in Pellet Culture System, Iranian Journal of Basic Medical Sciences (2008)
20. Comparison of Tgf-Β3 and Avocado/Soybean Unsaponifiable on Chondrogenesis of Human Adipose-Derived Stem Cells on Poly (Lactic-Co-Glycolic) Acid/ Hyaluronic Acid Hybrid Scaffold, Iranian Journal of Basic Medical Sciences (2020)
21. Chondrogenesis of Mesenchymal Stromal Cells on the 3D Printed Polycaprolactone/Fibrin/Decellular Cartilage Matrix Hybrid Scaffolds in the Presence of Piascledine, Journal of Biomaterials Science, Polymer Edition (2024)
22. Chondrogenic Activity of Two Herbal Products; Pomegranate Fruit Extract and Avocado/Soybean Unsaponifiable, Research in Pharmaceutical Sciences (2020)
23. Comparison of Aggrecan Gene Expression in Chondrogenesis of Adipose-Derived Stem Cells in Pellet and Micromass Culture Systems, Tehran University Medical Journal (2018)
24. Incorporation of Chitosan Nanoparticles Into Silk Fibroin-Based Porous Scaffolds: Chondrogenic Differentiation of Stem Cells, International Journal of Polymeric Materials and Polymeric Biomaterials (2016)
25. Access to Chondrocyte Culture, With Alginate, in Iran, Yakhteh (2008)
26. Comparing the Effects of Transforming Growth Factor Beta1 (Tgf-ß1) and Piascledine on the Expression of Collagen Ii, X and Aggrecan Genes in Chondrogenesis of Human Adipose-Derived Stem Cells in Fibrin Alginate Composite Scaffold, Journal of Isfahan Medical School (2016)
27. Hybrid and Composite Scaffolds Based on Extracellular Matrices for Cartilage Tissue Engineering, Tissue Engineering - Part B: Reviews (2019)
28. Evaluation Avocado Soybean Unsaponifiables Loaded in Poly (Lactic-Co-Glycolic) Acid/Avocado Soybean Unsaponifiables-Fibrin Nanoparticles Scaffold (New Delivery System) Is an Effective Factor for Tissue Engineering, Advanced Biomedical Research (2021)
29. An Animal Model Study of Osteochondral Defect Repair by Human Adipose Stem Cells and Pomegranate Fruit Hydroalchoholic Extract, Avicenna Journal of Phytomedicine (2023)
30. The Effect of High Frequency Electric Field on Enhancement of Chondrogenesis in Human Adipose-Derived Stem Cells, Iranian Journal of Basic Medical Sciences (2014)
31. A Comparative Study of Articular Chondrocytes Metabolism on a Biodegradable Polyesterurethane Scaffold and Alginate Beads in Different Oxygen Tension and Ph, Journal of Isfahan Medical School (2009)
32. Effect of Polyhydroxybutyrate/Chitosan/Bioglass Nanofiber Scaffold on Proliferation and Differentiation of Stem Cells From Human Exfoliated Deciduous Teeth Into Odontoblast-Like Cells, Materials Science and Engineering C (2018)
33. Stem Cells Therapy for Cartilage Regeneration in Clinic: Challenges and Opportunities, Cartilage: From Biology to Biofabrication (2023)
34. Comparative Study of Microtubule-Associated Protein-2 and Glial Fibrillary Acidic Proteins During Neural Induction of Human Bone Marrow Mesenchymal Stem Cells and Adipose-Derived Stem Cells, International Journal of Preventive Medicine (2014)
35. The Effects of Fibrin–Icariin Nanoparticle Loaded in Poly (Lactic‑Co‑Glycolic) Acid Scaffold As a Localized Delivery System on Chondrogenesis of Human Adipose‑Derived Stem Cells, Advanced Biomedical Research (2020)
36. Estrogen Treatment Enhances Neurogenic Differentiation of Human Adipose Derived Stem Cells in Vitro, Iranian Journal of Basic Medical Sciences (2015)
37. Overview of Retinal Differentiation Potential of Mesenchymal Stem Cells: A Promising Approach for Retinal Cell Therapy, Annals of Anatomy (2017)
38. Protective Effect of Soy Protein on Collagen-Induced Arthritis in Rat, Rheumatology International (2012)
39. Study of Carbon Nano-Tubes Effects on the Chondrogenesis of Human Adipose Derived Stem Cells in Alginate Scaffold, International Journal of Preventive Medicine (2014)
40. Effect of T3 Hormone on Neural Differentiation of Human Adipose Derived Stem Cells, Cell Biochemistry and Function (2014)
41. Study of Human Chondrocyte Redifferntiation Capacity in Three-Dimensional Hydrogel Culture, Iranian Journal of Basic Medical Sciences (2008)
42. An Animal Model Study for Repair of Tracheal Defects With Autologous Stem Cells and Differentiated Chondrocytes From Adipose-Derived Stem Cells, Journal of Pediatric Surgery (2012)
43. Comparing the Effect of Silk Fibroin-Based Scaffolds on Differentiation of Rabbit Chondrocytes, Journal of Isfahan Medical School (2014)
44. The Effects of Tgf-Β3 on the Proliferation and Function of Encapsulated Costal Cartilage Chondrocytes in Alginate Scaffold, Journal of Applied Biotechnology Reports (2019)
45. The Effect of Testosterone on the Amount of Platelet Derived Growth Factor and Stromal Cell-Derived Factors-1Alpha in Bone Marrow of Male Wistar Rats, Journal of Isfahan Medical School (2013)