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The Effect of Pomegranate Extract on Producing Type Ii Collagen in Differentiation of Adipose-Derived Stem Cells Into Chondrocytes



Katani M1 ; Zolfaghari B2 ; Soleimani M1 ; Valiani A1 ; Hashemibeni B1
Authors
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Authors Affiliations
  1. 1. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences AND Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2018

Abstract

Background: Cartilage tissue is avascular and has no repairing capacity. For cartilage tissue engineering, stem cells and growth factors are used. In this study, the effect of pomegranate as inducer for chondrogenesis of adipose-derived stem cells (ADSCs) was evaluated. Methods: Human adipose-derived stem cells in third passage seeded in fibrin were cultured in chondrogenic medium with pomegranate for 2 weeks. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay) and western blot technique were applied for evaluation of differentiated cells. Findings: Adipose-derived stem cells differentiated into chondrocytes; and type II collagen production by differentiated cells was proved. Conclusion: Pomegranate extract is an appropriate inducer for production of type II collagen in adipose-derived stem cells. © 2018, Isfahan University of Medical Sciences(IUMS). All rights reserved.
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