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Evolocumab, a Pcsk9 Inhibitor, Protects Human Endothelial Cells Against H2o2-Induced Oxidative Stress Publisher Pubmed

Summary: A study found evolocumab protects blood vessel cells from oxidative damage, potentially reducing heart disease risk. #HeartHealth #Antioxidants

Safaeian L1 ; Mirian M2 ; Bahrizadeh S1
Authors

Source: Archives of Physiology and Biochemistry Published:2022


Abstract

Context: Recent surveys have shown an association between proprotein convertase subtilisin/kexin type 9 (PCSK9) and oxidative stress. Objective: In this investigation, the effect of evolocumab an anti-PCSK9 antibody was assessed against oxidative damage caused by hydrogen peroxide (H2O2) in human umbilical vein endothelial cells (HUVEC). Material and methods: Viability of HUVEC was measured by MTT assay. Hydroperoxides and malondialdehyde (MDA) levels, and ferric reducing antioxidant power (FRAP) were detected in HUVEC that pre-treated with evolocumab and, then exposed to H2O2. Results: Evolocumab significantly prevented the cytotoxicity induced by H2O2 at the concentrations of 5–100 µg/ml. Pre-treatment of HUVEC with evolocumab reduced hydroperoxides and MDA levels and also increased FRAP value in intra- and extra-cellular mediums compared with H2O2 stimulated cells at different concentration ranges. Conclusion: This study displayed anti-oxidative and cytoprotective activities of evolocumab against oxidative damage caused by H2O2 in endothelial cells. © 2020 Informa UK Limited, trading as Taylor & Francis Group.
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