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Evaluation of Neural Gene Expression in Serum Treated Embryonic Stem Cells in Alzheimer's Patients



Dehghani L1 ; Hashemibeni B2 ; Poorazizi E1 ; Khorvash F3 ; Shaygannejad V3 ; Sedghi M4 ; Vesal S3 ; Meamar R1, 3
Authors
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Authors Affiliations
  1. 1. Department of Medical Sciences, Islamic Azad University, Najafabad Branch, Isfahan, Iran
  2. 2. Department of Anatomical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Isfahan Neurosciences Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Genetics Laboratory, Alzahra University Hospital, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Research in Medical Sciences Published:2013

Abstract

Background: Previous studies confirmed that neural gene expression in embryonic stem cells (ESC) could influence by chemical compounds through stimulating apoptotic pathway. We aimed to use ESCs-derived neural cells by embryoid body formation as an in vitro model for determination of neural gene expression changes in groups that treated by sera from Alzheimer's patients and compare with healthy individuals. Materials and Methods: ESC line which was derived from the C57BL/6 mouse strain was used throughout this study. ESC-derived neural cells were treated with serum from Alzheimer's patient and healthy individual. Neural gene expression was assessed in both groups by quantitative real-time polymerase chain reaction analysis. The data was analyzed by SPSS Software (version 18). Results: Morphologically, the reducing in neurite out-growth was observed in neural cells in group, which treated by serum from Alzheimer's patient, while neurite growth was natural in appearance in control group. Microtubule-associated protein 2 and glial fibrillary acidic protein expression significantly reduced in the Alzheimer's patient group compared with the control group. Nestin expression did not significantly differ among the groups. Conclusion: Neural gene expression could be reduced in serum treated ESC in Alzheimer's patients.
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