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Mesenchymal Stem/Stromal (Mscs)-Derived Exosome Inhibits Retinoblastoma Y-79 Cell Line Proliferation and Induces Their Apoptosis Publisher



Kheradjoo H1 ; Nouralishahi A2, 3 ; Firozabdi MSH4 ; Mohammadzadehsaliani S5
Authors
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Authors Affiliations
  1. 1. Department of Pathology, Imam Khomeini Hospital, Urmia University of Medical Sciences, Urmia, Iran
  2. 2. Isfahan Eye Research Center, Isfahan University of Medical sciences, Iran
  3. 3. KIMS Health Hospital, Oman, Oman
  4. 4. Master of Science Graduated from Shahid Beheshti Medical University
  5. 5. Department of Ophthalmology, Rustaq Hospital, Rustaq, Oman

Source: Nanomedicine Research Journal Published:2022


Abstract

Objective(s): Based on the literature, mesenchymal stem/stromal cell-derived exosome has a dual role in tumor therapy or progress. We evaluated the possible anti-tumor effect of the MSCs-exosome on retinoblastoma Y-79 cell cells in vitro. Methods: To verify the efficient isolation of exosomes, the MSCs-exosome structure and the expression of CD9 and CD81 were examined using transmission electron microscopy (TEM) and western blotting, respectively. Y-79 cell proliferation and apoptosis were evaluated using MTT assay and flow cytometry. The Real-time PCR estimated the expression levels of Bcl-2 and microRNA-143. Results: MSCs-exosome inhibited Y-79 cell proliferation as shown by MTT assay results. Also, MSCs-exosome induced apoptosis in Y-79 cells within 72 hours of treatment. Real-time PCR results also showed that exosome therapy decreased Bcl-2 expression while up-regulating microRNA-143 expression. Conclusions: MSCs-derived exosomes could deter retinoblastoma Y-79 cells tumorigenicity, thereby providing a novel modality to manage retinoblastoma patients. © Nanomedicine Research Journal 2022. All rights reserved.
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