Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Evaluation of Mg63 Cells Behavior With Electrospun Nanocomposite Scaffolds of Polycaprolactone and Carbon Quantum Dot Containing Captopril for Bone Tissue Engineering Publisher



Ghorghi M1 ; Gharavi AM2 ; Rafirenia M3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Student Research Committee, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
  3. 3. Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Knowledge and Health in Basic Medical Sciences Published:2020


Abstract

Introduction: Electrospinning method with the ability to make porous parts similar to bone matrix is a suitable method for making bone scaffolds. On the other hand, the use of drugs in inducing osteogenesis along with tissue engineering scaffolds is very important as a new approach in repairing damaged bone. The aim of the study was to evaluate the MG63 cells behavior with electrospun nanocomposite scaffolds of polycaprolactone and carbon quantum dot containing captopril for bone tissue engineering. Methods: The microstructure of synthesized CQDs was evaluated by both transmission electron microscopy (TEM). The microstructure and hydrophilicity/hydrophobicity ratio of scaffolds were assessed by scanning electron microscopy (SEM) and wettability test, respectively. The mechanical strength of the scaffolding was measured using a tensile test. The cell viability, attachment, proliferation, and alkaline phosphatase (ALP) activity of scaffolds were assessed using MG-63 cell line in vitro. Based on our results, the scaffold containing CQDs and CP led to a significant increase in the cells’ proliferation and ALP activity. Results: The results of cell culture showed an increase in the growth of MG-63 cells on nanocomposite scaffolds containing 0.5% by weight of quantum dot carbon and 10% captopril compared to other scaffolds. In addition, a significant increase in alkaline phosphatase secretion on nanocomposite scaffolds containing 0.5% by weight of quantum dot carbon and 10% of captopril was quite evident. Conclusion: Therefore, the PCL/CQDs/CP scaffold has a promising potential for bone tissue regeneration. © 2020, Shahroud University of Medical Sciences. All rights reserved.
Related Docs
View other Related Docs
1. Electrospun Captopril-Loaded Pcl-Carbon Quantum Dots Nanocomposite Scaffold: Fabrication, Characterization, and in Vitro Studies, Polymers for Advanced Technologies (2020)
2. Tunable Cellular Interactions and Physical Properties of Nanofibrous Pcl-Forsterite: Gelatin Scaffold Through Sequential Electrospinning, Composites Science and Technology (2013)
3. The Electrospun Poly(Ε-Caprolactone)/Fluoridated Hydroxyapatite Nanocomposite for Bone Tissue Engineering, Polymers for Advanced Technologies (2020)
4. Evaluation of the Effects of Starch on Polyhydroxybutyrate Electrospun Scaffolds for Bone Tissue Engineering Applications, International Journal of Biological Macromolecules (2021)
5. Effects of Nano-Bioactive Glass on Structural, Mechanical and Bioactivity Properties of Poly (3-Hydroxybutyrate) Electrospun Scaffold for Bone Tissue Engineering Applications, Materials Technology (2019)
6. Synthetic Electrospun Nanofibers As a Supportive Matrix in Osteogenic Differentiation of Induced Pluripotent Stem Cells, Journal of Biomaterials Science, Polymer Edition (2022)
7. Extracellular Matrix Coated Three-Dimensional-Printed Polycaprolactone Scaffolds Containing Curcumin for Cartilage Tissue Engineering Applications, Journal of Bioactive and Compatible Polymers (2024)
8. Development of Porous Photopolymer Resin-Swcnt Produced by Digital Light Processing Technology Using for Bone Femur Application, Archives of Bone and Joint Surgery (2021)
Experts (# of related papers)
View all Related Experts
Saeed Karbasi (101)
Mohammad Rafienia (67)
Other Related Docs
9. Polyhydroxybutyrate-Starch/Carbon Nanotube Electrospun Nanocomposite: A Highly Potential Scaffold for Bone Tissue Engineering Applications, International Journal of Biological Macromolecules (2022)
10. Fabrication of 3D Porous Polyurethane-Graphene Oxide Scaffolds by a Sequential Two-Step Processing for Non-Load Bearing Bone Defects, Physica Scripta (2024)
11. In Vitro Bioactivity of Baghdadite-Coated Pcl –Graphene Nanocomposite Scaffolds: Mechanism of Baghdadite and Apatite Formation, Materials Technology (2020)
12. A Femoral Shape Porous Scaffold Bio-Nanocomposite Fabricated Using 3D Printing and Freeze-Drying Technique for Orthopedic Application, Materials Chemistry and Physics (2022)
13. Evaluation of Physical, Mechanical and Biological Properties of Bioglass/Titania Scaffold Coated With Poly (3-Hydroxybutyrate)-Chitosan for Bone Tissue Engineering Applications, Materials Technology (2020)
14. Evaluation of Physical, Mechanical and Biological Properties of Β-Tri-Calcium Phosphate/Poly-3-Hydroxybutyrate Nano Composite Scaffold for Bone Tissue Engineering Application, Materials Technology (2021)
15. Preparing Nanocomposite Fibrous Scaffolds of P3hb/Nha for Bone Tissue Engineering, 2010 17th Iranian Conference of Biomedical Engineering, ICBME 2010 - Proceedings (2010)
16. Preparation and Characterization of Silk-Chitosan Composite As a Three- Dimensional Tool for Culturing Osteoblast-Like Cells, Journal of Isfahan Medical School (2015)
17. Global Criterion Optimization Method for Improving the Porosity of Porous Scaffolds Containing Magnetic Nanoparticles: Fabrication and Finite Element Analysis, Materials Science and Engineering: B (2023)
18. Novel Bilayer Electrospun Poly(Caprolactone)/ Silk Fibroin/ Strontium Carbonate Fibrous Nanocomposite Membrane for Guided Bone Regeneration, Journal of Applied Polymer Science (2021)
19. Cartilage Particles Can Promote Chondrogenesis of Adipose-Derived Stromal Cells on Poly(Ε-Caprolactone)/Fibrin Hybrid Constructs Prepared Via Sandwich Model, Journal of Biomimetics, Biomaterials and Biomedical Engineering (2020)
20. Effects of Decellularized Extracellular Matrix on Polyhydroxybutyrate Electrospun Scaffolds for Cartilage Tissue Engineering, Polymer-Plastics Technology and Materials (2023)
21. The Influence of Bioglass Nanoparticles on the Biodegradation and Biocompatibility of Poly (3-Hydroxybutyrate) Scaffolds, International Journal of Artificial Organs (2012)
22. Effect of Cellulose Nanofibers on Polyhydroxybutyrate Electrospun Scaffold for Bone Tissue Engineering Applications, International Journal of Biological Macromolecules (2022)
23. Adipose-Derived Stem Cells Growth and Proliferation Enhancement Using Poly (Lactic-Co-Glycolic Acid) (Plga)/Fibrin Nanofiber Mats, Journal of Applied Biotechnology Reports (2021)
24. Fabrication of Gehlenite Nanopowder Containing Electrospun Nanofibers for Bone Tissue Engineering, Fibers and Polymers (2021)
25. Surface Modification of Additive Manufactured Ti6al4v Scaffolds With Gelatin/Alginate- Igf-1 Carrier: An Effective Approach for Healing Bone Defects, International Journal of Biological Macromolecules (2024)
26. Investigation of Physical, Mechanical and Biological Properties of Polyhydroxybutyrate-Chitosan/Graphene Oxide Nanocomposite Scaffolds for Bone Tissue Engineering Applications, International Journal of Biological Macromolecules (2023)
27. Pepgen-P15 Delivery to Bone: A Novel 3D Printed Scaffold for Enhanced Bone Regeneration, Journal of Drug Delivery Science and Technology (2024)
28. A Ternary Nanocomposite Fibrous Scaffold Composed of Poly(Ε-Caprolactone)/Gelatin/Gehlenite (Ca2al2sio7): Physical, Chemical, and Biological Properties in Vitro, Polymers for Advanced Technologies (2021)
29. Investigating of Physical, Mechanical, and Biological Properties of Polyhydroxybutyrate-Keratin/Alumina Electrospun Scaffold Utilized in Bone Tissue Engineering, Materials Chemistry and Physics (2023)
30. Zn-Substituted Mg2sio4 Nanoparticles-Incorporated Pcl-Silk Fibroin Composite Scaffold: A Multifunctional Platform Towards Bone Tissue Regeneration, Materials Science and Engineering C (2021)
31. Does the Tissue Engineering Architecture of Poly(3-Hydroxybutyrate) Scaffold Affects Cell-Material Interactions?, Journal of Biomedical Materials Research - Part A (2012)
32. Characterization of Poly(Ε-Caprolactone)/Extracellular Matrix Nanofibers Composite Scaffold for Tissue Engineering, Journal of Isfahan Medical School (2019)
33. In Vitro Studies of Polycaprolactone Nanofibrous Scaffolds Containing Novel Gehlenite Nanoparticles, Journal of Medical Signals and Sensors (2021)
34. Casein Release and Characterization of Electrospun Nanofibres for Cartilage Tissue Engineering, Bulletin of Materials Science (2022)
35. Assessing Physicochemical, Mechanical, and in Vitro Biological Properties of Polycaprolactone/Poly(Glycerol Sebacate)/Hydroxyapatite Composite Scaffold for Nerve Tissue Engineering, Materials Chemistry and Physics (2022)
36. Calcitonin-Loaded Octamaleimic Acid–Silsesquioxane Nanoparticles in Hydrogel Scaffold Support Osteoinductivity in Bone Regeneration, Pharmaceutical Development and Technology (2021)
37. Development of Electrospun Poly (Vinyl Alcohol)-Based Bionanocomposite Scaffolds for Bone Tissue Engineering, Journal of Biomedical Materials Research - Part A (2018)
38. Incorporation of Inorganic Bioceramics Into Electrospun Scaffolds for Tissue Engineering Applications: A Review, Ceramics International (2022)
39. Polycaprolactone-Chitosan/Multi-Walled Carbon Nanotube: A Highly Strengthened Electrospun Nanocomposite Scaffold for Cartilage Tissue Engineering, International Journal of Biological Macromolecules (2022)
40. Preparation and Characterization of 3D Nanocomposite Scaffold From Bioactive Glass/Β-Tricalcium Phosphate Via Robocasting Method for Bone Tissue Engineering, Journal of Non-Crystalline Solids (2022)
41. Polycaprolactone/Gelatin/Hydroxyapatite Nanocomposite Scaffold Seeded With Stem Cells From Human Exfoliated Deciduous Teeth to Enhance Bone Repair: In Vitro and in Vivo Studies, Materials Technology (2022)
42. Preparation and Characterization of Poly Ε-Caprolactone-Gelatin/Multi-Walled Carbon Nanotubes Electrospun Scaffolds for Cartilage Tissue Engineering Applications, International Journal of Polymeric Materials and Polymeric Biomaterials (2020)
43. Incorporation of Nanohydroxyapatite and Vitamin D3 Into Electrospun Pcl/Gelatin Scaffolds: The Influence on the Physical and Chemical Properties and Cell Behavior for Bone Tissue Engineering, Polymers for Advanced Technologies (2018)
44. Sodium Alginate/Magnesium Oxide Nanocomposite Scaffolds for Bone Tissue Engineering, Polymers for Advanced Technologies (2018)
45. Highly Porous 3D Printed Scaffold Incorporated With Graphene Oxide-Merwinite and Coated With Igf1 Loaded Nanofibers for Calvarial Defect Repair, Journal of Polymers and the Environment (2024)
46. Poly (Glycerol Sebacate) and Polyhydroxybutyrate Electrospun Nanocomposite Facilitates Osteogenic Differentiation of Mesenchymal Stem Cells, Journal of Drug Delivery Science and Technology (2021)
47. A New Approach to Fabrication of Cs/Bg/Cnt Nanocomposite Scaffold Towards Bone Tissue Engineering and Evaluation of Its Properties, Applied Surface Science (2015)
48. Magnesium-Zinc-Graphene Oxide Nanocomposite Scaffolds for Bone Tissue Engineering, Arabian Journal of Chemistry (2023)
49. Synergetic Effect of Bioglass and Nano Montmorillonite on 3D Printed Nanocomposite of Polycaprolactone/Gelatin in the Fabrication of Bone Scaffolds, International Journal of Biological Macromolecules (2024)
50. Fabrication and Characterization of Two-Layered Nanofibrous Membrane for Guided Bone and Tissue Regeneration Application, Materials Science and Engineering C (2017)