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Incorporation of Nanohydroxyapatite and Vitamin D3 Into Electrospun Pcl/Gelatin Scaffolds: The Influence on the Physical and Chemical Properties and Cell Behavior for Bone Tissue Engineering Publisher



Sattary M1 ; Khorasani MT2 ; Rafienia M3 ; Rozve HS4
Authors
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Authors Affiliations
  1. 1. Department of Biomedical Engineering, Science, and Research Branch, Islamic Azad University, Tehran, Iran
  2. 2. Biomaterial Department of Iran Polymer and Petrochemical Institute, PO Box 14965, Tehran, 159, Iran
  3. 3. Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan, 81744176, Iran
  4. 4. Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 81744176, Iran

Source: Polymers for Advanced Technologies Published:2018


Abstract

Scaffold, an essential element of tissue engineering, should provide proper physical and chemical properties and evolve suitable cell behavior for tissue regeneration. Polycaprolactone/Gelatin (PCL/Gel)-based nanocomposite scaffolds containing hydroxyapatite nanoparticles (nHA) and vitamin D3 (Vit D3) were fabricated using the electrospinning method. Structural and mechanical properties of the scaffold were determined by scanning electron microscopy (SEM) and tensile measurement. In this study, smooth and bead-free morphology with a uniform fiber diameter and optimal porosity level with appropriate pore size was observed for PCL/Gel/nHA nanocomposite scaffold. The results indicated that adding nHA to PCL/Gel caused an increase of the mechanical properties of scaffold. In addition, chemical interactions between PCL, gelatin, and nHA molecules were shown with XRD and FT-IR in the composite scaffolds. MG-63 cell line has been cultured on the fabricated composite scaffolds; the results of viability and adhesion of cells on the scaffolds have been confirmed using MTT and SEM analysis methods. Here in this study, the culture of the osteoblast cells on the scaffolds showed that the addition of Vit D3 to PCL/Gel/nHA scaffold caused further attachment and proliferation of the cells. Moreover, DAPI staining results showed that the presence and viability of the cells were greater in PCL/Gel/nHA/Vit D3 scaffold than in PCL/Gel/nHA and PCL/Gel scaffolds. The results also approved increasing cell proliferation and alkaline phosphatase (ALP) activity for MG-63 cells cultured on PCL/Gel/nHA/Vit D3 scaffold. The results indicated superior properties of hydroxyapatite nanoparticles and vitamin D3 incorporated in PCL/Gel scaffold for use in bone tissue engineering. Copyright © 2017 John Wiley & Sons, Ltd.
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