Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Investigation of the Expression of P-Element-Induced Wimpy Testis-Interacting Rnas in Human Acute Myeloid Leukemia Publisher



Ghaseminezhad Z1 ; Sharifi M1 ; Bahreini A2 ; Mehrzad V3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, PA, United States
  3. 3. Department of Internal Medicine, Division of Hematology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Meta Gene Published:2022


Abstract

P-element induced wimpy testis (PIWI)-interacting RNAs (piRNAs) are a newly known class of small non-coding RNAs with 24–31 nucleotides in length. There are more than 20,000 piRNA genes in the human genome and some of them have a role in cancer. In this study, we investigated the expression of piRNAs in acute myeloid leukemia (AML). We used publicly available small RNA sequencing data derived from the peripheral blood of patients with AML and control samples to be re-analyzed for investigation of piRNA expression pattern and then was validated via the real-time polymerase chain reaction (PCR). Differential expression analysis showed that four upregulated piRNAs and six downregulated piRNAs in AML samples, among which, the piR-32,877 and piR-33,195 were the top two upregulated piRNAs. Real-time PCR was performed to validate the increased expression of these two piRNAs in AML compared to controls. Pathway analysis was also performed. The results provide evidence that piR-32,877 and piR-33,195 may serve as a new diagnostic and prognostic biomarker in AML. © 2021 Elsevier B.V.
Related Docs
View other Related Docs
1. Effects of Hsa-Pir-32877 Suppression With Antisense Lna Gapmers on the Proliferation and Apoptosis of Human Acute Myeloid Leukemia Cells, International Journal of Molecular and Cellular Medicine (2023)
2. Differential Expression and Pathological Implications of Linc01089 and Linc01578 in Acute Myeloid Leukemia, Gene Reports (2025)
3. Circulating Lncrna Hotair Is a Biomarker for Pediatric Acute Lymphoblastic Leukemia and Mediator of Mir-326 Exosomal Export, Scientific Reports (2025)
4. Knockdown of Long Noncoding Rna Plasmacytoma Variant Translocation 1 With Antisense Locked Nucleic Acid Gapmers Exerts Tumor-Suppressive Functions in Human Acute Erythroleukemia Cells Through Downregulation of C-Myc Expression, Cancer Biotherapy and Radiopharmaceuticals (2019)
5. New Long Noncoding Rna Biomarkers and Cerna Networks on Mir‑616‑3P in Colorectal Cancer: Bioinformatics‑Based Study, Journal of Research in Medical Sciences (2024)
6. Circular Rnas: Pivotal Role in the Leukemogenesis and Novel Indicators for the Diagnosis and Prognosis of Acute Myeloid Leukemia, Frontiers in Oncology (2023)
7. Microrna Dysregulation and Its Impact on Apoptosis-Related Signaling Pathways in Myelodysplastic Syndrome, Pathology Research and Practice (2024)
8. Comprehensive Competitive Endogenous Rna Network Analysis Reveals Ezh2-Related Axes and Prognostic Biomarkers in Hepatocellular Carcinoma, Iranian Journal of Basic Medical Sciences (2022)
Experts (# of related papers)
View all Related Experts
Mohammadreza Sharifi (16)
Mohammad Kazemi (3)
Other Related Docs
9. Single Cell Rna Sequencing Improves the Next Generation of Approaches to Aml Treatment: Challenges and Perspectives, Molecular Medicine (2025)
10. Downregulation of Mir100hg Induces Apoptosis in Human Megakaryoblastic Leukemia Cells, Indian Journal of Hematology and Blood Transfusion (2021)
11. Evaluation of Silencing Effect of Mir-133A-5P Mimic on Tim-3 Expression in Aml (Hl-60) Cell Line, Indian Journal of Clinical Biochemistry (2020)
12. Evaluation of P21cip1 and P27kip1 Expression in De Novo Acute Lymphoblastic Leukemia Patients, Biomedical Research and Therapy (2018)
13. The Effect of Decitabine on the Expression and Methylation of the Ppp1ca, Btg2, and Pten in Association With Changes in Mir-125B, Mir-17, and Mir-181B in Nalm6 Cell Line, Journal of Cellular Biochemistry (2019)
14. Transcription Analysis of a Histones Modifiers Panel Coupled With Critical Tumor Suppressor Genes Displayed Frequent Changes in Patients With Aml.: Mrna Levels of Histones Modifiers and Tsgs in Aml, Current Research in Translational Medicine (2021)
15. Dysregulated of the Fam138e and Clrn1-As1 Lncrnas Could Be Diagnosis Biomarkers in Colorectal Cancer, Human Gene (2024)
16. Inhibition of Microrna Mir-92A Inhibits Cell Proliferation in Human Acute Promyelocytic Leukemia; [Mikrorna Mir-92A Inhibisyonu Insan Akut Promyelositik Losemide Hucre Proliferasyonunu Inhibe Eder], Turkish Journal of Hematology (2013)
17. Lncrna Flvcr1-As1 Sponges Mir-381-3P and Promotes Wnt Signaling Pathway Resulting in Colorectal Cancer Progression, Egyptian Journal of Medical Human Genetics (2025)
18. Evaluation of the Effect of Tim-3 Suppression by Mir-498 and Its Effect on Apoptosis and Proliferation Rate of Hl-60 Cell Line, Pathology Research and Practice (2018)
19. State of the Art in Microrna As Diagnostic and Therapeutic Biomarkers in Chronic Lymphocytic Leukemia, Journal of Cellular Physiology (2018)
20. Mir-574, Mir-499, Mir-125B, Mir-106A, and Mir-9 Potentially Target Tgfbr-1 and Tgfbr-2 Genes Involving in Inflammatory Response Pathway: Potential Novel Biomarkers for Chronic Lymphocytic Leukemia, Pathology Research and Practice (2022)
21. The Effect of Long Non-Coding Rna Pvt1 Inhibition by Antisense Lna Gapmers Technology on Proliferation of Human Acute Erythroleukemia Cells, Journal of Isfahan Medical School (2018)
22. The Mir-125A-3P Inhibits Tim-3 Expression in Aml Cell Line Hl-60 in Vitro, Indian Journal of Hematology and Blood Transfusion (2017)
23. Apoptosis Induction in Acute Promyelocytic Leukemia Cells Through Upregulation of Cebpα by Mir-182 Blockage, Molecular Biology Research Communications (2018)
24. Inducing Cell Proliferative Prevention in Human Acute Promyelocytic Leukemia by Mir-182 Inhibition Through Modulation of Casp9 Expression, Biomedicine and Pharmacotherapy (2017)
25. Reduce Proliferation of Human Bone Marrow Cells From Acute Myeloblastic Leukemia With Minimally Differentiation by Blocking Lncrna Pvt1, Clinical and Translational Oncology (2020)
26. Antiproliferative Effect of Upregulation of Hsa-Let-7C-5P in Human Acute Erythroleukemia Cells, Cytotechnology (2018)
27. Circulating Microrna-192 As a Diagnostic Biomarker in Human Chronic Lymphocytic Leukemia, Cancer Gene Therapy (2016)
28. Inducing Apoptosis and Decreasing Cell Proliferation in Human Acute Promyelocytic Leukemia Through Regulation Expression of Casp3 by Let-7A-5P Blockage, Indian Journal of Hematology and Blood Transfusion (2018)
29. Inhibition of Microrna Mir-92A Induces Apoptosis and Inhibits Cell Proliferation in Human Acute Promyelocytic Leukemia Through Modulation of P63 Expression, Molecular Biology Reports (2014)
30. Microrna in Leukemia: Tumor Suppressors and Oncogenes With Prognostic Potential, Journal of Cellular Physiology (2019)
31. Blockage of Mir-92A-3P With Locked Nucleic Acid Induces Apoptosis and Prevents Cell Proliferation in Human Acute Megakaryoblastic Leukemia, Cancer Gene Therapy (2016)
32. Differential Expression Profile of Mir-27B, Mir-29A, and Mir-155 in Chronic Lymphocytic Leukemia and Breast Cancer Patients, Molecular Therapy Oncolytics (2020)
33. Induction of Apoptosis and Necrosis in Human Acute Erythroleukemia Cells by Inhibition of 47Tlong Non-Coding Rna Pvt1, Molecular Biology Research Communications (2018)
34. Construction of a Cell Cycle-Specific Lncrna-Mirna-Mrna Network Reveals Novel Key Lncrnas in Colorectal Cancer, Biomarkers (2024)
35. Mrna Overexpression of Baalc: A Novel Prognostic Factor for Pediatric Acute Lymphoblastic Leukemia, Biomedical Reports (2015)