Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Mir-125A-3P Inhibits Tim-3 Expression in Aml Cell Line Hl-60 in Vitro Publisher



Emamdoost F1 ; Khanahmad H2 ; Ganjalikhanihakemi M3 ; Doosti A1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Genetics, Faculty of Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
  2. 2. Department of Genetics, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Immunology Department, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Indian Journal of Hematology and Blood Transfusion Published:2017


Abstract

Acute Myeloid Leukemia is a cancer of leukemic stem cells (LSCs) with a rapid progression. It is characterized by overproduction of immature myeloid cells in bone marrow which crowds out normal hematopoietic stem cells (HSC). TIM-3, an immune regulatory molecule, is an LSC specific surface marker in AML with high expression on these cells compared to HSCs. Studies have indicated that micro RNAs (miRNAs) may play an important role in either cancer progression or suppression. Based on bioinformatics assessments, we have predicted that miR-125a-3p could be a miRNA with high suppressive activity on TIM-3 expression. The purpose of this study was to investigate the inhibitory effect of miR-125a-3p on TIM-3 gene expression in an AML cell line, HL-60, in vitro. HL-60 cells were cultured in RPMI 1640 supplied with 10 % FBS. TIM-3 expression was induced on the cells using phorbol miristate acetate. The cells were transfected with miR-125-3p for 24 h and the gene and protein expression of TIM-3 were measured using q-RT-PCR and flow-cytometery methods, respectively. The results of this study showed that miR-125a-3p has a strong silencing effect on TIM-3 gene and protein expression on HL-60 cell line. Based to our results, miR125a-3p can strongly silence TIM-3 expression in AML cell line. Thus, our results have confirmed the bioinformatics prediction of suppressive effect of miR-125a-3p on TIM-3with Mirwalk and Target Scan softwares. © 2016, Indian Society of Haematology & Transfusion Medicine.
Related Docs
View other Related Docs
1. Negative Regulation of Tim-3 Expression in Aml Cell Line (Hl-60) Using Mir-330-5P, British Journal of Biomedical Science (2016)
2. Evaluation of Silencing Effect of Mir-133A-5P Mimic on Tim-3 Expression in Aml (Hl-60) Cell Line, Indian Journal of Clinical Biochemistry (2020)
3. Evaluation of the Effect of Tim-3 Suppression by Mir-498 and Its Effect on Apoptosis and Proliferation Rate of Hl-60 Cell Line, Pathology Research and Practice (2018)
4. Silencing of Tim-3 Expression by Mir-326 Affects Apoptosis and Proliferation of Human Hl-60 Leukemia Cell Line, UHOD - Uluslararasi Hematoloji-Onkoloji Dergisi (2018)
5. Tim-3 in Leukemia; Immune Response and Beyond, Frontiers in Oncology (2021)
6. Tim-3/Gal-9 Interaction Affects Glucose and Lipid Metabolism in Acute Myeloid Leukemia Cell Lines, Frontiers in Immunology (2023)
7. Blockage of Mir-92A-3P With Locked Nucleic Acid Induces Apoptosis and Prevents Cell Proliferation in Human Acute Megakaryoblastic Leukemia, Cancer Gene Therapy (2016)
8. Apoptosis Induction in Acute Promyelocytic Leukemia Cells Through Upregulation of Cebpα by Mir-182 Blockage, Molecular Biology Research Communications (2018)
Experts (# of related papers)
View all Related Experts
Mohammadreza Sharifi (31)
Mazdak Ganjalikhani Hakemi (15)
Other Related Docs
9. Study of T-Cell Immunoglobulin and Mucin Domain-3 Expression Profile in Peripheral Blood and Bone Marrow of Human Acute Lymphoblastic Leukemia Patients, Journal of Research in Medical Sciences (2020)
10. The Role of Bone Marrow Microenvironment (Bmm) Cells in Acute Myeloid Leukemia (Aml) Progression: Immune Checkpoints, Metabolic Checkpoints, and Signaling Pathways, Cell Communication and Signaling (2023)
11. Micrornas Regulating Wnt Signaling Pathway in Colorectal Cancer: Biological Implications and Clinical Potentials, Functional and Integrative Genomics (2022)
12. Dysregulated Expression and Functions of Microrna-330 in Cancers: A Potential Therapeutic Target, Biomedicine and Pharmacotherapy (2022)
13. Induction of Apoptosis and Necrosis in Human Acute Erythroleukemia Cells by Inhibition of 47Tlong Non-Coding Rna Pvt1, Molecular Biology Research Communications (2018)
14. Differential Expression Profile of Mir-27B, Mir-29A, and Mir-155 in Chronic Lymphocytic Leukemia and Breast Cancer Patients, Molecular Therapy Oncolytics (2020)
15. Microrna in Leukemia: Tumor Suppressors and Oncogenes With Prognostic Potential, Journal of Cellular Physiology (2019)
16. Inhibition of Microrna Mir-92A Induces Apoptosis and Inhibits Cell Proliferation in Human Acute Promyelocytic Leukemia Through Modulation of P63 Expression, Molecular Biology Reports (2014)
17. Antiproliferative Effect of Upregulation of Hsa-Let-7C-5P in Human Acute Erythroleukemia Cells, Cytotechnology (2018)
18. Reduce Proliferation of Human Bone Marrow Cells From Acute Myeloblastic Leukemia With Minimally Differentiation by Blocking Lncrna Pvt1, Clinical and Translational Oncology (2020)
19. Inducing Cell Proliferative Prevention in Human Acute Promyelocytic Leukemia by Mir-182 Inhibition Through Modulation of Casp9 Expression, Biomedicine and Pharmacotherapy (2017)
20. Inhibition of Microrna Mir-92A Inhibits Cell Proliferation in Human Acute Promyelocytic Leukemia; [Mikrorna Mir-92A Inhibisyonu Insan Akut Promyelositik Losemide Hucre Proliferasyonunu Inhibe Eder], Turkish Journal of Hematology (2013)
21. Downregulation of Mir100hg Induces Apoptosis in Human Megakaryoblastic Leukemia Cells, Indian Journal of Hematology and Blood Transfusion (2021)
22. Effects of Hsa-Pir-32877 Suppression With Antisense Lna Gapmers on the Proliferation and Apoptosis of Human Acute Myeloid Leukemia Cells, International Journal of Molecular and Cellular Medicine (2023)
23. Microrna Dysregulation and Its Impact on Apoptosis-Related Signaling Pathways in Myelodysplastic Syndrome, Pathology Research and Practice (2024)
24. Circulating Lncrna Hotair Is a Biomarker for Pediatric Acute Lymphoblastic Leukemia and Mediator of Mir-326 Exosomal Export, Scientific Reports (2025)
25. Differential Expression and Pathological Implications of Linc01089 and Linc01578 in Acute Myeloid Leukemia, Gene Reports (2025)
26. Single Cell Rna Sequencing Improves the Next Generation of Approaches to Aml Treatment: Challenges and Perspectives, Molecular Medicine (2025)
27. Mir-370 Expression Analysis in Breast Cancer, Journal of Isfahan Medical School (2015)
28. Connection of Mir-185 and Mir-320A Expression Levels With Response to Interferon-Beta in Multiple Sclerosis Patients, Multiple Sclerosis and Related Disorders (2020)
29. The Correlation Between Flt3-Itd Mutation in Dendritic Cells With Tim-3 Expression in Acute Myeloid Leukemia, Blood Science (2021)
30. Inhibition of Microrna Mir-222 With Lna Inhibitor Can Reduce Cell Proliferation in B Chronic Lymphoblastic Leukemia, Indian Journal of Hematology and Blood Transfusion (2017)
31. Apoptosis-Inducing and Antiproliferative Effect by Inhibition of Mir-182-5P Through the Regulation of Casp9 Expression in Human Breast Cancer, Cancer Gene Therapy (2017)
32. Evaluation of the Expressed Mir‑129 and Mir‑549A in Patients With Multiple Sclerosis, Advanced Biomedical Research (2021)
33. Locked Nucleic Acid Anti-Mir-21 Inhibits Cell Growth and Invasive Behaviors of a Colorectal Adenocarcinoma Cell Line: Lna-Anti-Mir As a Novel Approach, Cancer Gene Therapy (2016)
34. Degradation of Mir-21 Induces Apoptosis and Inhibits Cell Proliferation in Human Hepatocellular Carcinoma, Cancer Gene Therapy (2015)
35. Expression of Hsa-Mir-204, Runx2, Pparγ, and Bcl2 in Bone Marrow Derived Mesenchymal Stem Cells From, Cell Journal (2017)
36. Inhibition of Microrna-21 Via Locked Nucleic Acid-Anti-Mir Suppressed Metastatic Features of Colorectal Cancer Cells Through Modulation of Programmed Cell Death 4, Tumor Biology (2017)
37. Crosstalk of Transcriptional Regulators of Adaptive Immune System and Micrornas: An Insight Into Differentiation and Development, Cells (2023)
38. The Effect of Decitabine on the Expression and Methylation of the Ppp1ca, Btg2, and Pten in Association With Changes in Mir-125B, Mir-17, and Mir-181B in Nalm6 Cell Line, Journal of Cellular Biochemistry (2019)
39. Recent Insights Into the Microrna-Dependent Modulation of Gliomas From Pathogenesis to Diagnosis and Treatment, Cellular and Molecular Biology Letters (2022)
40. Transcription Analysis of a Histones Modifiers Panel Coupled With Critical Tumor Suppressor Genes Displayed Frequent Changes in Patients With Aml.: Mrna Levels of Histones Modifiers and Tsgs in Aml, Current Research in Translational Medicine (2021)
41. Mir-196: Emerging of a New Potential Therapeutic Target and Biomarker in Colorectal Cancer, Molecular Biology Reports (2020)
42. Modulation of Long Non-Coding Rnas and Micrornas by Quercetin As a Potential Therapeutical Approach in Cancer: A Comprehensive Review, Current medicinal chemistry (2025)
43. Semaphorin-3A As an Immune Modulator Is Suppressed by Microrna-145-5P, Cell Journal (2018)
44. Emerging Biologic and Clinical Implications of Mir-182-5P in Gynecologic Cancers, Clinical and Translational Oncology (2024)
45. The Role of Tim-3 in Hepatocellular Carcinoma: A Promising Target for Immunotherapy?, Frontiers in Oncology (2020)
46. Unveiling Mesenchymal Stem Cells’ Regenerative Potential in Clinical Applications: Insights in Mirna and Lncrna Implications, Cells (2023)
47. Microrna-200C Overexpression in Cancer-Associated Fibroblasts Reduces the Invasive Properties of Breast Tumour Cells, Clinical and Translational Discovery (2022)
48. Comparison of Mir-211 Expression in Murine Melanoma Cell Line and Murine Melanoma Tumor by Real-Time Polymerase Chain Reaction, Journal of Isfahan Medical School (2012)
49. The Significant Role of Micrornas in Gliomas Angiogenesis: A Particular Focus on Molecular Mechanisms and Opportunities for Clinical Application, Cellular and Molecular Neurobiology (2023)
50. Pathogenic and Therapeutic Roles of Cytokines in Acute Myeloid Leukemia, Cytokine (2021)