Immunomodulatory Effect of Cd71+ Erythroid Cells in Patients With Kidney Failure Publisher Pubmed



B Zamani BATOOL ; F Riazian FATEMEH ; Mm Taba Masoud MOEINI ; A Namdar AFSHIN ; Fz Barzoki Fateme ZAREEI ; A Atapour ABDOLAMIR ; H Motedayyen HOSSEIN
Source: BMC Nephrology Published:2025

Abstract

Background: Immune abnormalities play a critical role in the pathogenesis of kidney failure. CD71+ erythroid cells are known to modulate immune responses. However, their immunomodulatory effects in kidney failure patients remain unclear. Therefore, we investigated the frequencies of CD71 + erythroid cells, Th1, Th2, Th17 cells, and regulatory T cells (Tregs), as well as the impact of CD71 + erythroid cells on the proliferation of peripheral blood mononuclear cells (PBMCs) of kidney failure patients. Methods: PBMCs were isolated from 49 kidney failure patients and 20 healthy subjects. The frequencies of T cell subsets and CD71 + erythroid cells were determined by flow cytometry. The numbers of reticulocytes and RBCs were also measured. Panel-reactive antibodies screening was used to assess antibodies against human leukocyte antigens (HLAs) in the patients. The effect of CD71 + erythroid cells on PBMC proliferation in kidney failure patients was investigated using a CFSE labeling assay. Erythropoietin level was measured by ELISA. Results: Kidney failure patients showed a significant increase in CD71 + erythroid cells (P < 0.01). In contrast, the frequencies of Th1, Th2, and Th17 cells were significantly reduced in these patients (P < 0.01–0.05). Among CD4 + T subsets, Th1 cell frequency was inversely correlated with the number of CD71 + erythroid cells (P < 0.05). In vitro, CD71 + erythroid cells inhibited the proliferation of PMBCs in kidney failure patients (P < 0.0001-0.05). No significant difference was observed in Treg frequencies between patients and controls. The frequency of CD71 + erythroid cells was significantly decreased in kidney failure patients with anti-HLA antibodies, unlike patients without anti-HLA antibodies (P < 0.001). The patients had an increased number of reticulocytes, unlike RBC, in peripheral blood compared with healthy subjects (P < 0.01–0.05). The patients exhibited a reduction in erythropoietin level compared with the control group (P < 0.0001). Conclusion: These findings suggest CD71 + erythroid cells may contribute to immune dysregulation in kidney failure patients, potentially playing a role in disease progression and its immunopathology. © 2025 Elsevier B.V., All rights reserved.