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Evaluation of Il-17 Gene Expression in Peripheral Blood and Sputum of Asthma Patients and Healthy Individuals



Yazdani R1 ; Hakemi MG2 ; Sherkat R3 ; Rezaei A1 ; Farahani R1 ; Beiranvand B4
Authors
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Authors Affiliations
  1. 1. Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Cellular and Molecular Immunology Research Center, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Acquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Biostatistics and Epidemiology, Kermanshah University of Medical Sciences, Kermanshah, Iran

Source: Tehran University Medical Journal Published:2014

Abstract

Background: Asthma as an airway disease is identified by increase network responsiveness of the trachea and bronchus to a specific stimulus. Th17 cells through produc-tion of IL-17 have important role in inflammation and autoimmune diseases. In some studies has been shown which IL-17 as major cytokine of Th17 probably has important role in the pathogenesis of allergy and asthma.; Methods: Total mRNA extracted from whole blood samples and sputum of 23 asthma patients and 23 normal controls. Then, total RNA was converted into cDNA according to the manufacturer’s instructions. Finally, the transcript levels of IL-17 were quantified by the real-time quantitative PCR. Twenty-three patients with asthma were diagnosed and selected according to the global initiative for asthma (GINA) and none of the patients had taken the medication at least three week before sampling. Healthy individuals did not have any history of allergy, asthma and other inflammatory diseases at the time of sampling. All of experiments have done in Isfahan University of Medical Sciences, Iran during May to February, 2013.; Results: This study showed a significant increase in transcript levels of IL-17 in the blood (287±79 versus 1/18±0/13) and sputum samples of the patients (64±28 versus 0/9±0/1) in comparison with normal individuals (P= 0.000, P= 0.029 respectively). It also revealed that the expression levels of the cytokines in the serum samples of the asthmatics were significantly more than their levels in patient’s sputum samples (P= 0.000). However, there was no significant difference between the cytokines expression levels in serum samples and sputum samples of the controls (P> 0.05).; Conclusion: In this study, we showed which the expression of IL-17 was increased in serum and sputum of asthmatic patients compared to healthy controls, this could result in elevation of neutrophils population and activation of pulmonary neutrophil. © 2014, Tehran University of Medical Sciences. All rights reserved.
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