Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Evaluation of Antibacterial Efficiency of Chitosan and Chitosan Nanoparticles on Cariogenic Streptococci: An in Vitro Study



Aliasghari A1 ; Khorasgani MR1 ; Vaezifar S2 ; Rahimi F1 ; Younesi H3 ; Khoroushi M4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Biology, Faculty of Sciences, University of Isfahan, Iran
  2. 2. Department of Medical Engineering, Payame Noor University, Iran
  3. 3. Department of Environmental Science, Faculty of Natural Resources, Tarbiat Modares University, Tehran, Iran
  4. 4. Dental Materials Research Center and Department of Operative Dentistry, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Iranian Journal of Microbiology Published:2016

Abstract

Background and Objectives: The most prevalent and worldwide oral disease is dental caries that affects a significant proportion of the world population. There are some classical approaches for control, prevention and treatment of this pathologic condition; however, the results are still not completely successful. Therefore new methods are needed for better management of this important challenge. Chitosan is a natural and non-toxic polysaccharide with many biological applications, particularly as an antimicrobial agent. Chitosan nanoparticle is a bioactive and environment friendly material with unique physicochemical properties. The aim of the present study was to investigate the antimicrobial effect of chitosan and nano-chitosan on the most important cariogenic streptococci. Materials and Methods: For evaluation of antimicrobial effect of chitosan and nano-chitosan against oral streptococci broth micro-dilution method was carried out for four bacterial species; Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguis and Streptococcus salivarius. Also the effect of these materials on adhesion of above bacteria was evaluated. One-way ANOVA and post hoc Tukey test were used for statistical analysis. Results: The MICs of chitosan for S. mutans, S. sanguis, S. salivarius and S. sobrinus were 1.25, 1.25, 0.625 and 0.625 mg/mL, respectively. The MIC of chitosan nanoparticle for S. mutans, S. salivarius and S. sobrinus was 0.625 mg/mL and for S. sanguis was 0.312 mg/mL. Chitosan and chitosan nanoparticles at a concentration of 5 mg/mL also reduced biofilm formation of S. mutans up to 92.5% and 93.4%, respectively. Conclusion: The results of this study supported the use of chitosan and chitosan nanoparticles as antimicrobial agents against cariogenic Streptococci. © 2016, Tehran University of Medical Science. All rights reserved.
Related Docs
View other Related Docs
1. Evaluation of the Effects of Chitosan Nanoparticles on Polyhydroxy Butyrate Electrospun Scaffolds for Cartilage Tissue Engineering Applications, International Journal of Biological Macromolecules (2023)
2. Stability Improvement of Immobilized Alkaline Phosphatase Using Chitosan Nanoparticles, Brazilian Journal of Chemical Engineering (2016)
3. Fabrication, Characterization and Antimicrobial Activity of Chitosan/Tragacanth Gum/Polyvinyl Alcohol Composite Films Incorporated With Cinnamon Essential Oil Nanoemulsion, International Journal of Biological Macromolecules (2023)
4. Preparation and Characterization of Gelatin/Chitosan Nanocomposite Reinforced by Nio Nanoparticles As an Active Food Packaging, Scientific Reports (2024)
5. The Promise of Chitosan Microspheres in Drug Delivery Systems, Expert Opinion on Drug Delivery (2007)
6. Preparation and Characterization of Trimethyl Chitosan /Hyaluronic Acid Composite Encapsulated With Ll37 to Promote Wound Healing in an Animal Model, International Journal of Peptide Research and Therapeutics (2023)
7. Preparation and in Vivo Evaluation of a Novel Gel-Based Wound Dressing Using Arginine–Alginate Surface-Modified Chitosan Nanofibers, Journal of Biomaterials Applications (2018)
8. Biological Evaluation of the Effects of Hyaluronic Acid on Poly (3-Hydroxybutyrate) Based Electrospun Nanocomposite Scaffolds for Cartilage Tissue Engineering Application, Materials Technology (2020)
Experts (# of related papers)
View all Related Experts
Jaleh Varshosaz (17)
Saeed Karbasi (6)
Other Related Docs
9. Evaluation of in Vivo Transfection Efficiency of Eudragit Coated Nanoparticles of Chitosan-Dna: A Ph-Sensitive System Prepared for Oral Dna Delivery, Iranian Red Crescent Medical Journal (2015)
10. Antibacterial Effect of Nano-Chlorhexidine on Enterococcus Faecalis Biofilm in Root Canal System:An in Vitro Study, Dental Research Journal (2022)
11. Chitosan-Titanium Dioxide-Glucantime Nanoassemblies Effects on Promastigote and Amastigote of Leishmania Major, International Journal of Biological Macromolecules (2018)
12. Nanobiomaterials in Periodontal Tissue Engineering, Nanobiomaterials in Hard Tissue Engineering: Applications of Nanobiomaterials (2016)
13. Fabrication and Evaluation of Cs/Pvp Sponge Containing Platelet-Rich Fibrin As a Wound Healing Accelerator: An in Vitro and in Vivo Study, International Journal of Biological Macromolecules (2022)
14. Nanocrystalline Cellulose-Hyaluronic Acid Composite Enriched With Gm-Csf Loaded Chitosan Nanoparticles for Enhanced Wound Healing, Biomedical Materials (Bristol) (2019)
15. Transferrin-Targeted Poly(Butylene Adipate)/Terephthalate Nanoparticles for Targeted Delivery of 5-Fluorouracil in Ht29 Colorectal Cancer Cell Line, Journal of Bioactive and Compatible Polymers (2017)
16. An Injectable Carboxymethyl Chitosan-Methylcellulose-Pluronic Hydrogel for the Encapsulation of Meloxicam Loaded Nanoparticles, International Journal of Biological Macromolecules (2020)
17. Study of Antibacterial Performance of Synthesized Silver Nanoparticles on Streptococcus Mutans Bacteria, Nanomedicine Research Journal (2022)
18. Anti-Nanobacterial Therapy for Prevention and Control of Periodontal Diseases, Dental Hypotheses (2010)
19. Enhanced Antibacterial Properties of Polyvinyl Alcohol/Starch/Chitosan Films With Nio–Cuo Nanoparticles for Food Packaging, Scientific Reports (2024)
20. Antimicrobial Efficacy of Antiplaque Agents of Common Toothpastes Against Porphyromonas Gingivalis and Streptococcus Oralis: An in Vitro Study, Dental Research Journal (2024)
21. Effect of Polymer Molecular Weight on Morphology and Particle Size of Chitosan Microspheres Prepared Via Spray Drying Method, IFMBE Proceedings (2009)
22. Local Delivery of Usnic Acid Loaded Rhamnolipid Vesicles by Gelatin / Tragacanth Gum / Montmorillonite/ Vanillin Cryogel Scaffold for Expression of Osteogenic Biomarkers and Antimicrobial Activity, Journal of Drug Delivery Science and Technology (2022)
23. Ciprofloxacin Loaded Alginate/Chitosan and Solid Lipid Nanoparticles, Preparation, and Characterization, Journal of Dispersion Science and Technology (2012)
24. Chitosan Microparticles Loaded With Exotoxin a Subunit Antigen for Intranasal Vaccination Against Pseudomonas Aeruginosa: An in Vitro Study, Carbohydrate Polymers (2011)
25. Physical and Biological Properties of Blend-Electrospun Polycaprolactone/Chitosan-Based Wound Dressings Loaded With N-Decyl-N, N-Dimethyl-1-Decanaminium Chloride: An in Vitro and in Vivo Study, Journal of Biomedical Materials Research - Part B Applied Biomaterials (2020)
26. Synthesis and Antibacterial Activity of Silver Doped Zinc Sulfide/Chitosan Bionanocomposites: A New Frontier in Biomedical Applications, International Journal of Biological Macromolecules (2024)
27. Production, Characterization, and Antimicrobial Activity of Almond Gum/Polyvinyl Alcohol/Chitosan Composite Films Containing Thyme Essential Oil Nanoemulsion for Extending the Shelf-Life of Chicken Breast Fillets, International Journal of Biological Macromolecules (2023)
28. Does Curcumin Have an Anticaries Effect? a Systematic Review of in Vitro Studies, Advances in Experimental Medicine and Biology (2021)
29. Evaluation of Physical, Mechanical, and Biodegradation of Chitosan/Graphene Oxide Composite As Bone Substitutes, Polymer-Plastics Technology and Materials (2020)
30. The Characterization and Antileishmanial Evaluation on Leishmania Major With Chitosan/Zno Bio-Nanocomposite As Drug Delivery Systems, Nanomedicine Research Journal (2022)
31. Immobilization of Alkaline Phosphatase Using Chitosan Nanoparticles for Enhancing Its Stability, Russian Journal of Applied Chemistry (2014)
32. Effects of Chitosan As a Novel Obturation Material for Pulpectomized Teeth on Periapical Inflammation, Periodontal Ligament Widening, and Hard Tissue Resorption: A Preliminary Exploratory Study on Dogs, Frontiers in Dentistry (2024)
33. Preparation, Characterization and Antibacterial Activities of Chitosan, N-Trimethyl Chitosan (Tmc) and N-Diethylmethyl Chitosan (Demc) Nanoparticles Loaded With Insulin Using Both the Ionotropic Gelation and Polyelectrolyte Complexation Methods, International Journal of Pharmaceutics (2008)
34. Carboxymethyl Cellulose-Based Wafer Enriched With Resveratrol-Loaded Nanoparticles for Enhanced Wound Healing, Drug Delivery and Translational Research (2020)
35. Mxene-Chitosan Composites and Their Biomedical Potentials, Micromachines (2022)
36. Polyelectrolyte Complexes of Chitosan for Production of Sustained Release Tablets of Bupropion Hcl, Farmacia (2015)
37. A Review of Challenges and Solutions of Biofilm Formation of Escherichia Coli: Conventional and Novel Methods of Prevention and Control, Food and Bioprocess Technology (2024)
38. Targeting Foodborne Pathogens Via Surface-Functionalized Nano-Antimicrobials, Advances in Colloid and Interface Science (2022)
39. Investigating the Effects of Chitosan/ Tragacanth Gum/ Polyvinyl Alcohol Composite Coating Incorporated With Cinnamon Essential Oil Nanoemulsion on Safety and Quality Features of Chicken Breast Fillets During Storage in the Refrigerator, International Journal of Biological Macromolecules (2023)
40. Polyurethane Nanocomposite Impregnated With Chitosan-Modified Graphene Oxide As a Potential Antibacterial Wound Dressing, Materials Science and Engineering C (2020)
41. Evaluation of the Effects of Β-Tricalcium Phosphate on Physical, Mechanical and Biological Properties of Poly (3-Hydroxybutyrate)/Chitosan Electrospun Scaffold for Cartilage Tissue Engineering Applications, Materials Technology (2019)
42. Comparison of Antibacterial Activity and Fluoride Release in Tooth-Colored Restorative Materials: Resin-Modified Glass Ionomer, Zirconomer, Giomer, and Cention N, Dental Research Journal (2022)
43. Preparation and Characterization of Hydroxyapatite Nanoparticles Doped With Nickel, Tin, and Molybdate Ions for Their Antimicrobial Effects, Drug Development and Industrial Pharmacy (2023)
44. Cytotoxicity Assessment of Polyhydroxybutyrate/Chitosan/Nano- Bioglass Nanofiber Scaffolds by Stem Cells From Human Exfoliated Deciduous Teeth Stem Cells From Dental Pulp of Exfoliated Deciduous Tooth, Dental Research Journal (2018)
45. Polycaprolactone/Chitosan Blend Nanofibers Loaded by 5-Fluorouracil: An Approach to Anticancer Drug Delivery System, Polymers for Advanced Technologies (2018)
46. Nanoparticles to Overcome Bacterial Resistance in Orthopedic and Dental Implants, Nanomedicine Research Journal (2022)
47. Physicochemical, Pharmaceutical and Biological Approaches Toward Designing Optimized and Efficient Hydrophobically Modified Chitosan-Based Polymeric Micelles As a Nanocarrier System for Targeted Delivery of Anticancer Drugs, Journal of Drug Targeting (2013)
48. Delivery of Antibacterial Agents for Wound Healing Applications Using Polysaccharide-Based Scaffolds, Journal of Drug Delivery Science and Technology (2023)
49. Application of Chitosan/Pva Nano Fiber As a Potential Wound Dressing for Streptozotocin-Induced Diabetic Rats, International Journal of Biological Macromolecules (2016)
50. Preparation and in Vitro Evaluation of Novel Cross-Linked Chondroitin Sulphate Nanoparticles by Aluminium Ions for Encapsulation of Green Tea Flavonoids, IET Nanobiotechnology (2018)