Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Morphological and Bactericidal Effects of Amikacin, Meropenem and Imipenem on Pseudomonas Aeruginosa Publisher



Moghoofei M1 ; Fazeli H1 ; Poursina F1 ; Esfahani BN1 ; Moghim S1 ; Vaez H1 ; Hadifar S1 ; Safaei HG1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, IR, Iran

Source: Jundishapur Journal of Microbiology Published:2015


Abstract

Background: Pseudomonas aeruginosa might be converted to coccoid bacteria under antibiotic stress. Bacterial conversion would increase resistance to antibiotics due to changes in cell wall crosslink or decreased metabolic activity. Morphology of P. aeruginosa under stress conditions (presence of antibiotics) can be changed to elongated bacilli, U shape and finally coccoid bacteria. Results of several researches showed that coccoid bacteria are one of the most important aspects of drug resistance. It would be the major reason for treatment failure. Objectives: The aim of this study was to determine in vitro morphological and bactericidal effects of amikacin, meropenem and imipenem on P. aeruginosa isolated from clinical specimens. Materials and Methods: Eight P. aeruginosa isolates obtained from clinical samples of burned patients and standard strain ATCC 27853 were used in this study. Isolates were identified by biochemical tests and confirmed by PCR method using ITS specific primer. Minimum inhibitory concentrations (MICs) of three antibiotics were determined by E-test method. Bacteria were exposed to antibiotics at different concentrations. Bacterial morphology in different days was examined by specific microscope and viability of isolates was examined by flow cytometry Results: All used antibiotics at sub MIC concentration had capability to induce coccoid bacteria. The highest rate of induced coccoid bacteria was 98.2% after 8 days, with contribution of imipenem and meropenem at 2 μg/mL concentration. Amikacin at 4 μg/mL concentration induced lower rate of coccoid bacteria (55.05%). Amikacin had a strong bactericidal effect on coccoid bacteria at 8 μg/mL concentration. Imipenem and meropenem showed very weak bactericidal effect on coccoid bacteria. Conclusions: Induction of coccoid form of P. aeruginosa may be one of the important reasons for antibiotic treatment failure; therefore, prescribed dose of antibiotics should be carefully managed to prevent increasing antibiotic resistance and coccoid bacteria induction. © 2015, Ahvaz Jundishapur University of Medical Sciences.
Related Docs
View other Related Docs
1. Identification of Beta-Lactamase-Producing Pseudomonas Aeruginosa With Multiple Antibiotic Resistances, Journal of Isfahan Medical School (2011)
2. Molecular Epidemiology and Mechanisms of Antimicrobial Resistance in Pseudomonas Aeruginosa Isolates Causing Burn Wound Infection in Iran, Journal of Chemotherapy (2014)
3. Resistance to Colistin and Detection of Mcr-1 Gene in Multidrug-Resistant Pseudomonas Aeruginosa Isolated From Wounds of the Hospitalized Burned Patients, Journal of Isfahan Medical School (2022)
4. Pattern of Antibiotic Resistance in Pesudomonas Aeruginosa Isolated From Intensive Care Unit, Isfahan, Iran, Journal of Isfahan Medical School (2013)
5. Prevalence of Blaveb and Blatem Genes, Antimicrobial Resistance Pattern and Biofilm Formation in Clinical Isolates of Pseudomonas Aeruginosa From Burn Patients in Isfahan, Iran, Gene Reports (2021)
6. Assessment of Phage Cocktails With Extended Host Range Activity Against Antibiotic Resistant Strains of Pseudomonas Aeruginosa, Comparative Clinical Pathology (2017)
7. Pseudomonas Aeruginosa Infections in Patients, Hospital Means, and Personnel's Specimens, Journal of Research in Medical Sciences (2012)
8. The Effect of Isolated Bacteriophage on Multi-Drug Resistant (Mdr) Pseudomonas Aeruginosa, Journal of Isfahan Medical School (2014)
Experts (# of related papers)
View all Related Experts
Hossein Fazeli (27)
Sharareh Moghim (19)
Other Related Docs
9. Plasmid Mediated Antibiotic Resistance in Isolated Bacteria From Burned Patients, Jundishapur Journal of Microbiology (2015)
10. The Study of Mutation in Lasr and Pqsr Genes in Extremely Drug Resistant and Multidrug Resistant Strains of Pseudomonas Aeruginosa From Burn Wound Infection, Jundishapur Journal of Microbiology (2019)
11. Investigating the Antagonistic Effect of Indigenous Probiotics on Carbapenem-Resistant Pseudomonas Aeruginosa Strains, BioMed Research International (2023)
12. Detection of Metallo-Beta Lactamases Among Carbapenem-Resistant Pseudomonas Aeruginosa, Jundishapur Journal of Microbiology (2014)
13. Epidemiology of Antibiotic Resistance Pattern of Pseudomonas Aeruginosa in Cystic Fibrosis Patients in Iran: A Systematic Review and Meta-Analysis, Gazi Medical Journal (2021)
14. Drug Resistance Pattern of Pseudomonas Aeruginosa Strains Isolated From Cystic Fibrosis Patients at Isfahan Al Zahra Hospital, Iran (2009-2010), Iranian Journal of Microbiology (2012)
15. Global Sequence Analysis and Expression of Azurin Gene in Different Clinical Specimens of Burn Patients With Pseudomonas Aeruginosa Infection, Infection and Drug Resistance (2020)
16. Determination of Biofilm Formation Ability and Antibiotic Resistance of Acinetobacter Baumannii Strains Isolated From Patients With Burn Wound Infection, Journal of Isfahan Medical School (2017)
17. Evaluation of the Synergistic Effect of Tomatidine With Several Antibiotics Against Standard and Clinical Isolates of Staphylococcus Aureus, Enterococcus Faecalis, Pseudomonas Aeruginosa and Escherichia Coli, Iranian Journal of Pharmaceutical Research (2017)
18. The Determination of Metallo-Beta-Lactamase Enzymes Prevalence in Pseudomonas Aeruginosa Using Etest and Their Antibiogram Patterns in Kermanshah, Iran, Journal of Kerman University of Medical Sciences (2015)
19. Evaluation of Pseudomonas Aeroginosa Isolated From Cutaneous Infections and Determination of Drug Resistance Pattern in Patients of Alzahra Hospital in Esfahan, Journal of Isfahan Medical School (2010)
20. Clonal Relatedness Among Imipenem-Resistant Pseudomonas Aeruginosa Isolated From Icu-Hospitalized Patients, Critical Care Research and Practice (2015)
21. Investigating of Four Main Carbapenem-Resistance Mechanisms in High-Level Carbapenem Resistant Pseudomonas Aeruginosa Isolated From Burn Patients, Journal of the Chinese Medical Association (2018)
22. Clonal Repetitive Element Polymerase Chain Reaction Patterns of Pseudomonas Aeruginosa in Diabetic Foot Ulcers, Iran, IJID Regions (2025)
23. Molecular Epidemiology of Colistin-Resistant Pseudomonas Aeruginosa Producing Ndm-1 From Hospitalized Patients in Iran, Iranian Journal of Basic Medical Sciences (2019)
24. Occurrence of Chlorine-Resistant Pseudomonas Aeruginosa in Hospital Water Systems: Threat of Waterborne Infections for Patients, Antimicrobial Resistance and Infection Control (2024)
25. Evaluation of Chlorogenic Acid and Carnosol for Anti-Efflux Pump and Anti-Biofilm Activities Against Extensively Drug-Resistant Strains of Staphylococcus Aureus and Pseudomonas Aeruginosa, Microbiology Spectrum (2024)
26. Antibiotic Resistance Patterns and Genetic Diversity in Clinical Isolates of Pseudomonas Aeruginosa Isolated From Patients of a Referral Hospital, Isfahan, Iran, Jundishapur Journal of Microbiology (2015)
27. Detection of Blactx-M15 and Blaoxa-48 Genes in Gram-Negative Isolates From Neonatal Sepsis in Central of Iran, Iranian Journal of Microbiology (2019)
28. Detection of Colistin Sensitivity in Clinical Isolates of Acinetobacter Baumannii in Iran, Journal of Research in Medical Sciences (2014)
29. Molecular Detection of Type Ii Toxin-Antitoxin Systems and Their Association With Antibiotic Resistance and Biofilm Formation in Clinical Acinetobacter Baumannii Isolates of Burn Patients, Journal of Mazandaran University of Medical Sciences (2021)
30. Investigation of Antimicrobial Susceptibility, Class I and Ii Integrons Among Pseudomonas Aeruginosa Isolates From Hospitalized Patients in Isfahan, Iran, BMC Research Notes (2018)
31. Detection of Morphotyping Characteristics Identification Antibiotic Resistance of Pseudomonas Aeruginosa Isolated Form Cystic Fibrosis Patient, Journal of Isfahan Medical School (2012)
32. The Emergence of Blaoxa-48 and Blandm Among Esbl-Producing Klebsiella Pneumoniae in Clinical Isolates of a Tertiary Hospital in Iran, Acta Microbiologica et Immunologica Hungarica (2018)
33. Identification of Genomic Species Ofacinetobacter Isolated From Burns of Icu Patients, Archives of Iranian Medicine (2015)
34. Evolutionary Trajectories of Beta-Lactamase Ndm and Dlst Cluster in Pseudomonas Aeruginosa: Finding the Putative Ancestor, Pathogens and Global Health (2024)
35. Molecular Characterization and Phage Typing of Methicillin-Resistant Staphylococcus Aureus Isolated From Clinical Samples in Isfahan, Iran, Jundishapur Journal of Microbiology (2023)
36. The Prevalence of Fluoroquinolone Resistant Genes in Clinical Isolates of Pseudomonas Aeruginosa of Plasmid Origin Isolated From Patients Admitted to Alzahra Hospital of Isfahan, Journal of Isfahan Medical School (2022)
37. The Bacterial Biofilm Resistome in Drinking Water Distribution Systems: A Systematic Review, Chemosphere (2023)
38. Isolation of Asian Endemic and Livestock Associated Clones of Methicillin Resistant Staphylococcus Aureus From Ocular Samples in Northeastern Iran, Iranian Journal of Microbiology (2013)
39. Characterization of Staphylococcus Epidermidis Isolates From Hospitalized Patients in Isfahan and Tehran Teaching Hospitals, Molecular Genetics, Microbiology and Virology (2014)
40. In Vitro Synergistic Effect of Colistin and Ampicillin/Sulbactam With Several Antibiotics Against Clinical Strains of Multi-Drug Resistant Acinetobacter Baumannii, Indian Journal of Medical Microbiology (2021)
41. The Antimicrobial Activity of Ferula Gummosa on Bacterial Strains Isolated From Patients With Gastroenteritis, Iranian Journal of Clinical Infectious Diseases (2011)
42. Detection of Icaad Gene and Biofilm Formation in Staphylococcus Aureus Isolates From Wound Infections, Iranian Journal of Public Health (2006)
43. Frequency of Meca Gene and Borderline Oxacillin Resistant Staphylococcus Aureus in Nosocomial Acquired Methicillin Resistance Staphylococcus Aureus Infections, Pakistan Journal of Biological Sciences (2008)
44. Identification of Blaoxa−51−23−58, Blavim, Blandm, and Blaimp Carbapenemase Genes in Acinetobacter Baumannii Isolates From Hospitalized Patients, BMC Research Notes (2024)
45. Prevalence and Molecular Identification of Antibiotic Resistant Airborne Bacteria at Intensive Care Units, Koomesh (2018)
46. High Prevalence of Multidrug Resistance and Metallo-Beta-Lactamase (Mβl) Producing Acinetobacter Baumanniiisolated From Patients in Icu Wards, Hamadan, Iran, Journal of Research in Health Sciences (2013)
47. Molecular Detection of Extended-Spectrum Β-Lactamases (Esbls) and Biofilm Formation in Uropathogen Klebsiella Pneumoniae in Iran, Medical Journal of the Islamic Republic of Iran (2021)
48. Assessment of Cage and Baba Mrna Expression During Morphological Conversion of Helicobacter Pylori From Spiral to Coccoid, Current Microbiology (2013)
49. From the Urinary Catheter to the Prevalence of Three Classes of Integrons, Β -Lactamase Genes, and Differences in Antimicrobial Susceptibility of Proteus Mirabilis and Clonal Relatedness With Rep-Pcr, BioMed Research International (2021)
50. Characterization and in Vitro Probiotic Assessment of Potential Indigenous Bacillus Strains Isolated From Soil Rhizosphere, Minerva Biotecnologica (2016)