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New Diterpene Polyester and Phenolic Compounds From Pycnocycla Spinosa Decne. Ex Boiss With Relaxant Effects on Kcl-Induced Contraction in Rat Ileum Publisher



Ghanadian M1 ; Sadraei H2 ; Yousuf S3 ; Asghari G1, 2 ; Choudhary MI3 ; Jahed M1, 2
Authors
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Authors Affiliations
  1. 1. Department of Pharmacognosy, Isfahan Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan

Source: Phytochemistry Letters Published:2014


Abstract

Using a bioassay-directed fractionation of Pycnocycla spinosa Decne. Ex Boiss. var. spinosa, a new polycyclic diterpenoid, 3,7,10,14,15-pentaacetyl-5- butanoyl-13,17-epoxy-8-myrsinene (1), two known compounds; vanillin (2) and isoacetovanilon (3), and a new phenolic compound, 6-(4-hydroxy-3-methoxyphenyl)- hexanoic acid (4) with inhibitory effects on KCl-induced smooth muscle contractions on the rat isolated ileum were obtained. Compound 1, the most active of the series, which exerted potent antispasmodic activity with IC 50 value of IC50 = 0.062 ± 0.011 μM, is likely the main active ingredient of the extract. The structures of the isolated compounds were established based on 13C and 1H NMR as well as 2D NMR, IR, HR-MS, and X-ray crystallographic methods. © 2013 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.
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