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St6gal1, Cox-2 and Hb-Egf Mrna Expression in Breast Cancer Samples From Kashan, Iran



Taherian A1 ; Zadeh SMM2 ; Ghani H3 ; Khamechian T4
Authors
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Authors Affiliations
  1. 1. Kashan Anatomical Research Center, Kashan University of Medical Sciences, Kashan, Iran
  2. 2. Microbiology Department, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Internal Medicine, Kashan University of Medical Sciences, Kashan, Iran
  4. 4. Kashan Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran

Source: Middle East Journal of Cancer Published:2015

Abstract

Background: ST6Gal1, Cox-2 and HB-EGF genes are involved in different tumors and their enhanced expressions often correlate with poor prognosis. In this study we assay the expressions of these genes by reverse transcriptase-PCR in 54 breast cancer samples.; Methods: Tissue samples were either formalin-fixed for histopathological examination or frozen for reverse transcriptase-PCR. Image program was used for the densitometry of the image of the gels and the expression of different genes was normalized with beta actin expression. The student’s t-test and correlation matrix were used for data analyses.; Results: We observed significantly higher expressions of ST6Gal1 (P= 0.040), Cox-2 (P= 0.001) and HB-EGF (P= 0.009) in the tumor region compared to the margin samples. A significant correlation was found between HB-EGF and Cox-2 expression (P= 0.001). There was a positive correlation between total score, tumor size, histology grade and nuclear grade but there was a reverse correlation between age and tumor size, histology grade and total score.; Conclusion: Expressions of ST6Gal1, Cox-2 and HB-EGF in breast tumor samples in this and a number of other studies emphasize their role as important markers in breast cancer. The use of medications to inhibit either their individual expressions or the possible inhibition of all three genes may improve patient survival and prevent metastasis. © 2015, Shiraz University of Medical Sciences. All rights reserved.
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