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Mir-195 Down-Regulation Is a Distinctive Biomarker of Her2 Positive State in Breast Cancer Publisher



Balmeh N1 ; Tabatabaeian H2, 3 ; Asgari M1 ; Mokhtarian R1 ; Abharian PH4 ; Azadeh M5 ; Ghaedi K2
Authors
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Authors Affiliations
  1. 1. Department of Cell and Molecular Biology, School of Biology, Noordanesh Institute of Higher Education, Meymeh, Iran
  2. 2. Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran
  3. 3. Anahid Breast Cancer Clinic, Isfahan Healthcare City, Isfahan, Iran
  4. 4. Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  5. 5. Zist-fanavari Novin Biotechnology Institute, Isfahan, Iran

Source: Gene Reports Published:2020


Abstract

Background: Breast cancer is the most common cancer among women worldwide. HER2-positive breast cancer accounts for nearly 20% of all cases. Upon targeted therapy resistance, these patients show fairly poor-prognosis. miRNAs have shown to be differentially expressed in HER2-positive cases that can decipher drug resistance. miR-195 down-regulation has been reported in breast cancer; however, very little is known about its expression particularly in HER2-positive breast cancers. Material and methods: qPCR was used to quantitatively measure the expression of miR-195 in normal, HER2-positive and HER2-negative fresh clinical samples. A computational approach was used to find unvalidated but potential targetome of miR-195. Results: miR-195 is down-regulated in breast cancer samples as compared to the normal controls. The level of down-regulation was significantly greater in HER2-positive tumors as compared to both normal and HER2-negative samples. Importantly, miR-195 down-regulation was demonstrated to be a potential diagnostic biomarker for distinguishing HER2-positive against both normal and HER2-negative samples. Besides, the in silico approach revealed that unvalidated miR-195 targetome play roles in oncogenic pathways related to breast tumorigenesis and progression. Conclusion: miR-195 is down-regulated in breast cancer, especially and significantly in HER2-positive cases. The expression of miR-195 could effectively be used as a distinctive diagnostic marker to discriminate HER2-positive from both normal and HER2-negative samples. © 2020 Elsevier Inc.
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