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Molecular Epidemiology and Antifungal Susceptibility Profile in Nakaseomyces Glabrata Species Complex: A 5-Year Countrywide Study Publisher Pubmed



Salimi M1, 2 ; Javidnia J2, 3 ; Faeli L1, 2 ; Moslemi A1, 2 ; Hedayati M2, 3 ; Haghani I2, 3 ; Aghili S2, 3 ; Moazeni M2, 3 ; Badiee P4 ; Roudbari M5 ; Zarrinfar H6 ; Mohammadi R7 ; Lotfali E8 ; Nouripoursisakht S9 Show All Authors
Authors
  1. Salimi M1, 2
  2. Javidnia J2, 3
  3. Faeli L1, 2
  4. Moslemi A1, 2
  5. Hedayati M2, 3
  6. Haghani I2, 3
  7. Aghili S2, 3
  8. Moazeni M2, 3
  9. Badiee P4
  10. Roudbari M5
  11. Zarrinfar H6
  12. Mohammadi R7
  13. Lotfali E8
  14. Nouripoursisakht S9
  15. Seyedmousavi S10
  16. Shokohi T2, 3
  17. Abastabar M2, 3
Show Affiliations
Authors Affiliations
  1. 1. Student Research Committee, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  2. 2. Invasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran
  3. 3. Department of Medical Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  4. 4. Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  5. 5. Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  6. 6. Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
  7. 7. Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  8. 8. Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  9. 9. Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
  10. 10. Microbiology Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, United States

Source: Journal of Clinical Laboratory Analysis Published:2024


Abstract

Background: The current study aimed to identify Iranian Nakaseomyces (Candida) glabrata complex species in the clinical isolates and determine their antifungal susceptibility profile. Methods: In total, 320 N. glabrata clinical isolates were collected from patients hospitalized in different geographical regions of Iran. The initial screening was performed by morphological characteristics on CHROMagar Candida. Each isolate was identified by targeting the D1/D2 rDNA using a multiplex-PCR method. To validate the mPCR method and determine genetic diversity, the ITS-rDNA region was randomly sequenced in 40 isolates. Additionally, antifungal susceptibility was evaluated against nine antifungal agents following the CLSI M27-A4 guidelines. Results: All clinical isolates from Iran were identified as N. glabrata. The analysis of ITS-rDNA sequence data revealed the presence of eight distinct ITS clades and 10 haplotypes among the 40 isolates of N. glabrata. The predominant clades identified were Clades VII, V, and IV, which respectively accounted for 22.5%, 17.5%, and 17.5% isolates. The widest MIC ranges were observed for voriconazole (0.016–8 μg/mL) and isavuconazole (0.016–2 μg/mL), whereas the narrowest ranges were seen with itraconazole and amphotericin B (0.25–2 μg/mL). Conclusion: Haplotype diversity can be a valuable approach for studying the genetic diversity, transmission patterns, and epidemiology of the N. glabrata complex. © 2024 The Author(s). Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.
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