In Vitro Induction Effect of 1,25(Oh)2D3 on Differentiation of Hair Follicle Stem Cell Into Keratinocyte Publisher Pubmed



Joulai Veijouyeh S^{1, 2} ; Mashayekhi F³ ; Yari A⁴ ; Heidari F⁵ ; Sajedi N⁶ ; Moghani Ghoroghi F⁷ ; Nobakht M^{1, 8, 9} Show Affiliations
Source: Biomedical Journal Published:2017

Abstract

Background Stem cells are characterized by self-renewal and differentiation capabilities. The bulge hair follicle stem cells (HFSCs) are able to convert to epithelial components. The active metabolite of vitamin D, 1,25(OH)2D3, plays important roles in this differentiation process. In the present study has found that 1,25(OH)2D3 induces the HFSCs differentiation into keratinocyte. Methods HFSCs are isolated from rat whiskers and cultivated in DMEM medium. To isolate bulge stem cell population, flow cytometry and immunocytochemistry using K15, CD34 and nestin biomarkers were performed. In order to accelerate the HFSCs differentiation into eratinocyte, HFSCs were treated with 10−12 M, 1,25(OH)2D3 every 48 h for a week. Results Immunocytochemistry results showed that bulge stem cells are nestin and CD34 positive but K15 negative before differentiation. Subsequently flow cytometry results, showed that the expression of nestin, CD34 and K15 were 70.96%, 93.03% and 6.88% respectively. After differentiation, the immunocytochemical and flow cytometry results indicated that differentiated cells have positive reaction to K15 with 68.94% expression level. Conclusion It was concluded that 10−12 M, 1,25(OH)2D3 could induce the HFSCs differentiation into keratinocytes. © 2017 Chang Gung University