The Effects of Coculture of Stem Cells From Human Exfoliated Deciduous Teeth (Shed) on K562 Tumor Cells



Gorji M¹ ; Hashemibeni B² ; Bahramian H² ; Salehi H² ; Alipour R³ ; Aliakbari M² Show Affiliations
Source: Journal of Isfahan Medical School Published:2014

Abstract

Background: Due to increased prevalence of cancer in human population, the effectiveness of chemotherapy and drug (therapy) is reducing because of recurrence and drug-resistance. Therefore, scientists are trying other options such as cell-therapy. Among the cells, scientists have more focused on evaluating effect of stem cells on tumor cells. Since there were controversial reports on this effect in literature, the aim of this study was to assess the proliferation and survival of K562 tumor cells cocultured with stem cells from human exfoliated deciduous teeth (SHED). Methods: Stem cells were isolated from human exfoliated deciduous teeth and cultured K562 cells were obtained from the Pasteur institute of Iran. Stem cells and K562 were directly cocultured with each other and the ratios of K562 to stem cells were 1/10, 10/1, and 1/1. In this study, the viability and proliferation of the cells via MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and trypan blue staining. Findings: The trypan blue and MTT assay results of 48 hours coculture with ratio of 1/10 in comparison with control group did not show any significance difference. But with this ratio, at 96 hour, proliferation of K562 compared to control had reduced significantly; and in other ratios (1/1 and 1/10) at 48 and 96 hours, proliferation of K562 increased significantly (P < 0.05). Conclusion: Results of our study revealed that in this coculture, increased number of stem cells had an inhibitory effect on proliferation of K562 cells. Moreover, low numbers of stem cells not only were not able to inhibit tumor cells, but also contributed to improve their proliferation. © 2014, Isfahan University of Medical Sciences(IUMS). All rights reserved.