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Effect of Strontium Ions Substitution on Gene Delivery Related Properties of Calcium Phosphate Nanoparticles Publisher Pubmed



Hanifi A1 ; Fathi MH1 ; Mir Mohammad Sadeghi H2
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Authors Affiliations
  1. 1. Biomaterials Group, Materials Engineering Department, Isfahan University of Technology, Isfahan 84156-83111, Iran
  2. 2. Biotechnology Department, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Materials Science: Materials in Medicine Published:2010


Abstract

Gene therapy has been considered a strategy for delivery of therapeutic nucleic acids to a specific site. Calcium phosphates are one gene delivery vector group of interest. However, low transfection efficiency has limited the use of calcium phosphate in gene delivery applications. Present work aims at studying the fabrication of strontium substituted calcium phosphate nanoparticles with improved gene delivery related properties. Strontium substituted calcium phosphate was prepared using a simple sol gel method. X-ray diffraction analysis, Fourier transform infrared spectroscopy, transmission electron microscopy, specific surface area analysis, zeta potential measurement and ion release evaluation were used to characterize the samples. This characterization showed strontium and carbonate co-substituted calcium phosphate which resulted in nano size particles with low crystallinity, high specific surface area, positive surface charge, and a high dissolution rate. These improved properties could increase the DNA concentration on the vector as well as the endosomal escape of the complex that leads to higher transfection efficiency of this novel gene delivery vector. © 2010 Springer Science+Business Media, LLC.
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