Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Gene Delivery by Pei-Nanocomplex Into Breast and Colorectal Tumor Cell Lines, the Impacts of N/P Ratio, Size and Type of the Cell; [Pei-Nanocomplex Tarafindan Meme Ve Kolorektal Tumor Hucre Hatlarinda Gen Dagitimi, N / P Oraninin, Hucrenin Boyutu Ve Turunun Etkileri]



Rasoolian M1 ; Hosseini SY2 ; Khanahmad H1 ; Sarvari J2 ; Rahbarizadeh F3 ; Amani AM4 ; Erfani N5 ; Kheirollahi M1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Bacteriology and Virology Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  3. 3. Department of Medical Biotechnology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  4. 4. Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
  5. 5. Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Source: Fabad Journal of Pharmaceutical Sciences Published:2020

Abstract

The safety, low cost, ease of preparation and manipulation of nonviral vectors such as PEIs (polyethyleneimines) have made them a widespread tool for gene delivery despite their poor efficiency compared to viral vectors. We used PEI in comparison with liposome reagent in combination with pEGFP-C1 to evaluate transfection efficiency and cytotoxicity in various cancer cell lines including MCF-7, SW-480, and CT-26. The N/P ratio (nitrogen groups of polymer/ phosphate groups of nucleic acid) 10:1 for PEI 2000 and PEI 25000 was selected as the most appropriate N/P ratio for transfection based on cell viability and transfection efficiency. The cell types received the nanoparticles and lipofectamines with different efficiencies according to the following relationship: MCF-7 (P<0.001)>SW-480 (P<0.001)>CT-26 (P<0.001). PEI 25000 acted as a non-viral vector better than PEI 2000 and lipofectamineTM 2000 (P<0.001) to transfect of pEGFP-C1 into MCF-7 breast cancer cell line, whereas PEI 2000 and lipofectamineTM 2000 were the better choice for plasmid transfection compared to PEI 25000 in SW-480 and CT-26 colorectal cancer cell lines. The results showed that by decreasing the size and increasing the charge of PEI 2000 and PEI 25000 nanoparticles the gene delivery was increased for colorectal cancer cell lines including SW-480 and CT-26. However, PEI 2000 and lipofectamine were more effective at entering colon cancer cells than PEI 25000. Increasing the size and charge associated with the nanoparticles, improved the expression level of GFP in MCF-7 breast cancer cells. Results of this study showed that evaluation of non-viral vectors on cancer cell types avoids wasting time and duplication studies. © 2020 Society of Pharmaceutical Sciences of Ankara (FABAD). All rights reserved.
Experts (# of related papers)
Other Related Docs
17. A Concise Review on Cancer Treatment Methods and Delivery Systems, Journal of Drug Delivery Science and Technology (2019)
18. Plga-Peg-Ra-Based Polymeric Micelles for Tumor Targeted Delivery of Irinotecan, Pharmaceutical Development and Technology (2018)
32. Lipoprotein Like Nanoparticles Used in Drug and Gene Delivery, Current Pharmaceutical Design (2016)
33. Advanced Nanosystems for Cancer Therapeutics: A Review, ACS Applied Nano Materials (2023)