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The Effect of Vitamin C-Loaded Electrospun Polycaprolactone/Poly (Glycerol Sebacate) Fibers for Peripheral Nerve Tissue Engineering Publisher



Alipour H1 ; Saudi A2 ; Mirazi H3 ; Kazemi MH4 ; Alavi O1 ; Zeraatpisheh Z5 ; Abolhassani S1 ; Rafienia M6
Authors
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Authors Affiliations
  1. 1. Department of Tissue Engineering and Applied Cell Sciences, School of Advance Medical Science and Technology, Shiraz University of Medical Sciences, Shiraz, Iran
  2. 2. Department of Biomaterials, Tissue Engineering and Nanotechnology, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Biomedical Engineering, Faculty of New Sciences and Technology, University of Tehran, Tehran, Iran
  4. 4. Department of Chemical Engineering, Isfahan University of Technology, Isfahan, 84156–83111, Iran
  5. 5. Epilepsy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  6. 6. Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Polymers and the Environment Published:2022


Abstract

Vitamin C (VC) is an essential supplement that plays a vital role in cellular processes and functions and has been applied for therapeutic purposes for many years. The beneficial effects of VC on peripheral nerve regeneration have been gained lots of attention. In this study, electrospun polycaprolactone (PCL)/polyglycerol sebacate (PGS) fibers incorporated with different concentrations of VC (5, 10, and 15 wt.%) were developed for peripheral nerve tissue engineering. The morphology of the fibers was investigated using scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FTIR), tensile analysis (Young’s modulus, ultimate tensile strength (UTS), and elongation at break), release profile of VC from the PCL/PGS fibers, in vitro degradation, water uptake behavior, and contact angle measurements were also studied. MTT assay and SEM were utilized to evaluate the attachment and viability of pheochromocytoma cells (PC12) on the scaffolds. The results showed that all scaffolds had a uniform diameter and mean diameter deceased from 1.24 to 0.88 µm followed by increasing VC. Young’s modulus and UTS enhanced with increasing in VC percentage. MTT assay demonstrated that PCL/PGS containing 5 wt.% VC had a greater viability rate among other scaffolds. Our outcome indicated possible applicability of VC containing scaffolds for nerve tissue engineering. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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