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The Ability of H2o2 to Induce Differentiation of Human Mesenchymal Stem Cells Into Chondrocytes



Goudarzi F1, 2 ; Mohammadalipour A3 ; Bahabadi M4 ; Moradi MN1 ; Sarveazad A5 ; Goodarzi MT6 ; Khodadadi I6
Authors
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Authors Affiliations
  1. 1. Students Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
  2. 2. Regenerative Medicine Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
  3. 3. Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Bioinformatics Research Center, Isfahan University of Medical Sciences, Iran
  4. 4. Department of Clinical Biochemistry, Faculty of Medicine, Qazvin University of Medical Sciences, Iran
  5. 5. Colorectal Research Center, Hazrat Rasoul Medical Complex, Iran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Biochemistry, Hamadan University of Medical Sciences, Hamadan, Iran

Source: Scientific Journal of Kurdistan University of Medical Sciences Published:2018

Abstract

Background and Aim: Cartilage disorders may deteriorate following oxidative stress injuries affecting mature chondrocytes. Meantime, mesenchymal stem cells (MSCs) can differentiate into chondrocytes in the presence of oxidative conditions and act as a source of compensation for injured chondrocytes. The present study aimed to investigate the effect of H2O2 on MSCs differentiation into chondrocytes in order to cast light on the dual roles of oxidative stress in the pathogenesis of diseases. Materials and Methods: Human mesenchymal stem cells were isolated from abdominal adipose tissue of three different donors and cultured in the presence of 50 μM H2O2 in order to differentiate into chondrocytes. We determined cell viability by tetrazolium assay and measured reactive oxygen species (ROS) level by flow cytometry. Presence of glycoseaminoglycans was confirmed by safranin staining. Results: The percentage of cells containing ROS was significantly higher in the cells treated with hydrogen peroxide (29.2% ± 1) compared to that in the untreated control cells (7.7% ± 1.4). A significant increase in glycoseaminoglycan content was observed in H2O2 treated cells compared to that in the control cells both on the 9th day (treated: 1.57×104 ± 0.1 vs control: 0.91×104 ± 0.09) and 21st day (treated: 2.87×104 ± 0.2 vs control: 0.96×104 ± 0.07). In addition, comparison of glycoseaminoglycan content on the 9th and 21st days showed a significantly higher content in both treated and control cells on the 21st day (p<0.05). Conclusion: Hydrogen peroxide resulted in increased differentiation of adipose tissue-derived MSCs into chondrocytes. Therefore, we concluded that, oxidative stress had positive role in the induction of chondrocyte differentiation. © 2018, Kurdistan University of Medical Sciences. All rights reserved.
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