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Incorporation of Chitosan Nanoparticles Into Silk Fibroin-Based Porous Scaffolds: Chondrogenic Differentiation of Stem Cells Publisher



Naeimi M1, 2 ; Rafienia M3 ; Fathi M2, 4 ; Janmaleki M5 ; Bonakdar S6 ; Ebrahimianhosseinabadi M7
Authors
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Authors Affiliations
  1. 1. Biomedical Engineering Department, Central Tehran Branch, Islamic Azad University, Tehran, Iran
  2. 2. Biomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran
  3. 3. Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan, 81744176, Iran
  4. 4. Dental Materials Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  5. 5. BioMEMS and Bioinspired Microfluidic Laboratory, Department of Mechanical and Manufacturing Engineering, University of Calgary, AB, Canada
  6. 6. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
  7. 7. Department of Biomedical Engineering, Faculty of Engineering, University of Isfahan, Isfahan, Iran

Source: International Journal of Polymeric Materials and Polymeric Biomaterials Published:2016


Abstract

A silk fibroin-chondroitin sulfate-sodium alginate (SF-CHS-SA) porous scaffold containing chitosan nanoparticles (NPs) was investigated. The proliferation of adipose-derived stem cells (ASCs) was studied by SEM, fluorescent microscopy, alcian blue staining, dimethylmethylene blue assay, and real-time polymerization chain reaction. The results showed that incorporation of NPs into the scaffold improved compressive modulus (5.6 ± 0.15 MPa). The amount of glycosaminoglycan expression of the ASCs was reached to 8.9 ± 0.3 g/mL. The gene expressions of aggrecan, collagen II, and SOX9 of the ASCs were significantly improved. This study revealed that the prepared scaffold can be used as a substrate for cartilage tissue engineering. © 2016 Taylor & Francis Group, LLC.
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