Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Effects of Chitosan-Loaded Jq1 Nanoparticles on Ovcar-3 Cell Cycle and Apoptosis-Related Gene Expression Publisher



Masoudi E1 ; Soleimani M1 ; Zarinfard G1 ; Homayoun M1 ; Bakhtiari M2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Anatomical Sciences, School of Medicine, Behbahan University of Medical Sciences, Behbahan, Iran

Source: Research in Pharmaceutical Sciences Published:2024


Abstract

Background and purpose: Ovarian cancer is the deadliest gynecological cancer. Bromodomain and extra terminal domain (BET) proteins play major roles in the regulation of gene expression at the epigenetic level. Jun Qi (JQ1) is a potent inhibitor of BET proteins. Regarding the short half-life and poor pharmacokinetic profile, JQ1 was loaded into newly developed nano-carriers. Chitosan nanoparticles are one of the best and potential polymers in cancer treatment. The present study aimed to build chitosan-JQ1 nanoparticles (Ch-J-NPs), treat OVCAR-3 cells with Ch-J-NPs, and evaluate the effects of these nanoparticles on cell cycle and apoptosis-associated genes. Experimental approach: Ch-J-NPs were synthesized and characterized. The size and morphology of Ch-J-NPs were defined by DLS and FE-SEM techniques. OVCAR-3 cells were cultured and treated with Ch-J-NPs. Then, IC50 was measured using MTT assay. The groups were defined and cells were treated with IC50 concentration of Ch-J-NPs, for 48 h. Finally, cells in different groups were assessed for the expression of genes of interest using quantitative RT-PCR. Findings/Results: IC50 values for Ch-J-NPs were 5.625 µg/mL. RT-PCR results demonstrated that the expression of genes associated with cell cycle activity (c-MYC, hTERT, CDK1, CDK4, and CDK6) was significantly decreased following treatment of cancer cells with Ch-J-NPs. Conversely, the expression of caspase-3, and caspase-9 significantly increased. BAX (pro-apoptotic) to BCL2 (anti-apoptotic) expression ratio, also increased significantly after treatment of cells with Ch-J-NPs. Conclusion and implications: Ch-J-NPs showed significant anti-cell cyclic and apoptotic effects on OVCAR-3 cells. © 2024 Wolters Kluwer Medknow Publications. All rights reserved.
Related Docs
View other Related Docs
1. In Vitro Evaluation of the Pogostone Effects on the Expression of Pten and Dact1 Tumor Suppressor Genes, Cell Cycle, and Apoptosis in Ovarian Cancer Cell Line, Research in Pharmaceutical Sciences (2022)
2. Anderson-Type Manganese Polyoxomolybdate Hybrid Nanocomposite for Boosting Drug Delivery Against Breast Cancer, Journal of Drug Delivery Science and Technology (2023)
3. Assessment of Chitosan Modified Nanoparticles, As Boron Carriers in Bnct, Journal of Mazandaran University of Medical Sciences (2018)
4. Boron Phenyl Alanine Targeted Chitosan–Pnipaam Core–Shell Thermo-Responsive Nanoparticles: Boosting Drug Delivery to Glioblastoma in Bnct, Drug Development and Industrial Pharmacy (2021)
5. Graphene Oxide Quantum Dot-Chitosan Nanotheranostic Platform As a Ph-Responsive Carrier for Improving Curcumin Uptake Internalization: In Vitro & in Silico Study, Biomaterials Advances (2022)
6. Pharmacokinetics and in Vitro/In Vivo Antitumor Efficacy of Aptamer-Targeted Ecoflex® Nanoparticles for Docetaxel Delivery in Ovarian Cancer, International Journal of Nanomedicine (2018)
7. Biodegradable and Biocompatible Subcutaneous Implants Consisted of Ph-Sensitive Mebendazole-Loaded/Folic Acid-Targeted Chitosan Nanoparticles for Murine Triple-Negative Breast Cancer Treatment, Journal of Nanobiotechnology (2022)
8. Co-Delivery of Carboplatin and Doxorubicin Using Zif-8 Coated Chitosan-Poly(N-Isopropyl Acrylamide) Nanoparticles Through a Dual Ph/Thermo Responsive Strategy to Breast Cancer Cells, International Journal of Biological Macromolecules (2024)
Experts (# of related papers)
View all Related Experts
Jaleh Varshosaz (8)
Mahboubeh Rostami (6)
Other Related Docs
9. Mxene-Chitosan Composites and Their Biomedical Potentials, Micromachines (2022)
10. Co-Delivery of Paclitaxel and Α-Tocopherol Succinate by Novel Chitosan-Based Polymeric Micelles for Improving Micellar Stability and Efficacious Combination Therapy, Drug Development and Industrial Pharmacy (2015)
11. Physicochemical, Pharmaceutical and Biological Approaches Toward Designing Optimized and Efficient Hydrophobically Modified Chitosan-Based Polymeric Micelles As a Nanocarrier System for Targeted Delivery of Anticancer Drugs, Journal of Drug Targeting (2013)
12. Can Estradiol and Ghrelin Play a Protective Role in Epithelial Ovarian Cancer Incidence in Postmenopausal Women?, Archives of Medical Research (2021)
13. Chitosan As Possible Inhibitory Agents and Delivery Systems in Leukemia, Cancer Cell International (2021)
14. Caveolae-Dependent Endocytosis Mediates the Cellular Uptake of Cdte Quantum Dots in Ovarian Cancer Cell Lines, Research in Pharmaceutical Sciences (2022)
15. Pegylated Trimethylchitosan Emulsomes Conjugated to Octreotide for Targeted Delivery of Sorafenib to Hepatocellular Carcinoma Cells of Hepg2, Journal of Liposome Research (2019)
16. Preparation of Chitosan Nanoparticles for Simultaneous Drug Delivery of Dacarbazine and Enoxaparin in Melanoma, Carbohydrate Polymers (2023)
17. Assessment of Oxaliplatin-Loaded Iodine Nanoparticles for Chemoradiotherapy of Human Colorectal Cancer (Ht-29) Cells, Polymers (2022)
18. What Are the Limitations of Chitosan Use in Non-Viral Gene Delivery and How to Overcome Them?, Biocompatible Nanomaterials: Synthesis, Characterization and Applications (2011)
19. Preparation and Characterization of Oligochitosan-Tragacanth Nanoparticles As a Novel Gene Carrier, Carbohydrate Polymers (2013)
20. Anti-Proliferative and Anti-Apoptotic Effects of Grape Seed Extract on Chemo-Resistant Ovcar-3 Ovarian Cancer Cells, Research in Pharmaceutical Sciences (2020)
21. Biodegradable Plga Implants Containing Doxorubicin-Loaded Chitosan Nanoparticles for Treatment of Breast Tumor-Bearing Mice, International Journal of Biological Macromolecules (2019)
22. Lignin, Lipid, Protein, Hyaluronic Acid, Starch, Cellulose, Gum, Pectin, Alginate and Chitosan-Based Nanomaterials for Cancer Nanotherapy: Challenges and Opportunities, International Journal of Biological Macromolecules (2021)