Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Stepwise Morphological Changes and Cytoskeletal Reorganization of Human Mesenchymal Stem Cells Treated by Short-Time Cyclic Uniaxial Stretch Publisher Pubmed



Parandakh A1 ; Tafazzolishadpour M1 ; Khani MM2, 3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Cardiovascular Engineering Lab, Faculty of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
  2. 2. Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Department of Tissue Engineering and Regenerative Medicine, School of Advanced technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: In Vitro Cellular and Developmental Biology - Animal Published:2017


Abstract

This study aimed to investigate stepwise remodeling of human mesenchymal stem cells (hMSCs) in response to cyclic stretch through rearrangement and alignment of cells and cytoskeleton regulation toward smooth muscle cell (SMC) fate in different time spans. Image analysis techniques were utilized to calculate morphological parameters. Cytoskeletal reorganization was observed by investigating F-actin filaments using immunofluorescence staining, and expression level of contractile SMC markers was followed by a quantitative polymerase chain reaction method. Applying cyclic uniaxial stretch on cultured hMSCs, utilizing a costume-made device, led to alteration in fractal dimension (FD) and cytoskeleton structure toward continuous alignment and elongation of cells by elevation of strain duration. Actin filaments became more aligned perpendicular to the axis of mechanical stretch by increasing uniaxial loading duration. At first, FD met a significant decrease in 4 h loading duration then increased significantly by further loading up to 16 h, followed by another decrease up to 1 d of uniaxial stretching. HMSCs subjected to 24 h cyclic uniaxial stretching significantly expressed early and intermediate contractile SM markers. It was hypothesized that the increase in FD after 4 h while cells continuously became more aligned and elongated was due to initiation of change in phenotype that influenced arrangement of cells. At this point, change in cell phenotype started leading to change in morphology while mechanical loading still caused cell alignment and rearrangement. Results can be helpful when optimized engineered cells are needed based on mechanical condition for functional engineered tissue and cell therapy. © 2017, The Society for In Vitro Biology.
Related Docs
1. The Effects of Short-Term Uniaxial Strain on the Mechanical Properties of Mesenchymal Stem Cells Upon Tgf-Β1 Stimulation, In Vitro Cellular and Developmental Biology - Animal (2018)
2. Morphology and Contractile Gene Expression of Adipose-Derived Mesenchymal Stem Cells in Response to Short-Term Cyclic Uniaxial Strain and Tgf-Β1, Biomedizinische Technik (2018)
3. Mechanical Characterization of Human Mesenchymal Stem Cells Subjected to Cyclic Uniaxial Strain and Tgf-Β1, Journal of the Mechanical Behavior of Biomedical Materials (2015)
4. Substrate Topography Interacts With Substrate Stiffness and Culture Time to Regulate Mechanical Properties and Smooth Muscle Differentiation of Mesenchymal Stem Cells, Colloids and Surfaces B: Biointerfaces (2019)
5. Effects of Cyclic Uniaxial Strain on Morphology of Mesenchymal Stem Cells During Differentiation to Smooth Muscle Cells, Koomesh (2016)
6. Correlation of the Cell Mechanical Behavior and Quantified Cytoskeletal Parameters in Normal and Cancerous Breast Cell Lines, Biorheology (2019)
7. Radiation Therapy Affects the Mechanical Behavior of Human Umbilical Vein Endothelial Cells, Journal of the Mechanical Behavior of Biomedical Materials (2018)