Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Evaluation of the Prevalence of Exons 1 and 10 Polymorphisms of Lhcgr Gene and Its Relationship With Ivf Success



Javadiarjmand M1 ; Damavandi E2, 3 ; Choobineh H4 ; Sarafraziesfandabadi F2 ; Kabuli M1 ; Mahdavi A5 ; Ghadami M1, 6, 7
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Specialized Medical Genetic Center of Academic Center for Education, Culture and Research (ACECR), Tehran University of Medical Sciences Branch, Tehran, Iran
  3. 3. Department of Photo Healing and Regeneration, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran
  4. 4. School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Obstetrics and Gynecology, Dr. Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Cardiac Primary Prevention Research Center, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Reproduction and Infertility Published:2019

Abstract

Background: Luteinizing hormone receptor gene shows four nonsynonymous polymorphisms within the exons. Three of these polymorphisms, i.e. rs4539842 (an insertion of 6bp CTGCAG at nucleotide position 54), rs12470652 (c.827A>G/p.Asn 291Ser), and rs2293275(c.935G>A/p.Ser312Asn) have been studied more frequently. Beside other hormones, LH and FSH have an important role in production of competent oocyte and female fertility. Therefore, the objective of the current study was to investigate the prevalence of exons 1(rs4539842) and 10(rs12470652, rs2293275) polymorphisms of the LHCGR gene and its relationship with successful IVF in Iranian infertile women. Methods: SNPs in exons 1 and 10 were analyzed in 100 women of two equally sized groups of IVF failure and IVF success women using genomic DNA. For polymorphisms in exon 10, PCR and direct sequencing were used and for the polymorphism in exon 1, RFLP technique was used. The RFLP technique is confirmed by sequencing. Results: Our results showed significant difference in allelic frequency of SNP rs2293275 among IVF successful and IVF failure groups (p=0.001). For this variation, AA genotype (A allele) was shown to have protective effect against IVF failure (p=0.03 and OR=0.04), while GG genotype (G allele) was a susceptive genotype to IVF failure (p=0.003 and OR=3.88).Allelic frequency of SNP rs4539842 also showed significant difference between the two groups (p=0.0025). For this SNP, subjects with no 6bp insertion (homozygote deletion genotype) were susceptible to failure in IVF (p=0.009 and OR=2.93). Conclusion: It has been revealed that two common SNPs (rs4539842 and rs2293275) in the LHCGR gene are associated with the outcome of IVF in Iranian infertile women. Thus, these two SNPs can be suggested to be used as predictors for IVF outcome in Iranian population. © 2019 Avicenna Research Institute. All rights reserved.
Related Docs
1. Influence of Psycho-Sexual Factors on the Quality of Life of Iranian Infertile Women: A Path Analysis, International Journal of Sexual Health (2020)
2. Differences in and Correlates of Sexual Function in Infertile Women With and Without Polycystic Ovary Syndrome, International Journal of Fertility and Sterility (2021)
3. Polycystic Ovarian Syndrome Elevates the Distress of Sexual Pain in Iranian Women With Infertility, BMC Women's Health (2024)
Experts (# of related papers)
Ashraf Moini (1)