Current and Emerging Techniques for Diagnosis of Toxoplasmosis in Pregnancy: A Narrative Review Publisher



A Teimouri AREF ; Sm Mahmoudi Shima MA ; A Behkar ATEFEH ; K Sahebi KEIVAN ; H Foroozand HOSSEIN ; G Hassanpour GHOLAMREZA ; Hv Keshavarz Valian Hossein VALIAN
Source: Iranian Journal of Parasitology Published:2024

Abstract

Toxoplasma gondii is an intracellular parasite capable of crossing the placenta in pregnancy and infecting the developing fetus, leading to various congenital anomalies and even abortion. Acute Toxoplasma infection is responsible for almost all cases of congenital toxoplasmosis in immunocompetent pregnant women. Prenatal screening for acute toxoplasmosis primarily involves maternal serology and fetal ultrasound imaging. When serological or ultrasound findings suggest acute infection, further diagnostic tests are necessary to confirm fetal infection. Currently, molecular methods to detect the para-site’s DNA, including polymerase chain reaction-based methods, on amniot-ic fluid are the gold standard tests for the diagnosis of congenital toxoplas-mosis. In this review, we aim to discuss various aspects of screening and diagnostic methods for toxoplasmosis in pregnancy, including (i) current serological assays, screening approaches, and future perspectives; (ii) the role of imaging techniques, with an emphasis on ultrasound; (iii) principles and recent advances in diagnostic molecular methods; (iv) emerging techniques, such as point-of-care-based tests and biosensors, and microRNAs as novel biomarkers of acute infection; and (v) an overview of screening programs in different countries, important epidemiological determinants, and recom-mendations for Toxoplasma screening health policies. © 2025 Elsevier B.V., All rights reserved.