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A High Sensitive Visible Light-Driven Photoelectrochemical Aptasensor for Shrimp Allergen Tropomyosin Detection Using Graphitic Carbon Nitride-Tio2 Nanocomposite Publisher Pubmed



Amouzadeh Tabrizi M1 ; Shamsipur M2 ; Saber R1, 3 ; Sarkar S1, 4 ; Ebrahimi V3
Authors
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Authors Affiliations
  1. 1. Research Center for Science and Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Chemistry, Razi University, Kermanshah, Iran
  3. 3. School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Iran
  4. 4. Department of Medical Physics and Biomedical Engineering Tehran University of Medical Sciences, Tehran, Iran

Source: Biosensors and Bioelectronics Published:2017


Abstract

Herein, for the first time a visible-light-driven photoelectrochemical (PEC) aptasensor for shrimp tropomyosin determination was fabricated by using graphitic carbon nitride (g-C3N4) and titanium dioxide (TiO2) as photoactive nanomaterials, ascorbic acid (AA) as electron donor and ruthenium (III) hexaammine (Ru(NH3)63+) as signal enhancer. The surface of an ITO electrode was first modified with g-C3N4, TiO2, and polyethyleneimine (PEI) and then the amine terminal aptamerTROP probe was attached to PEI by the use of glutaraldehyde (GA) as cross-linker. After that, Ru(NH3)63+ was adsorbed on aptamer to enhance the photocurrent signal. The principle of proposed PEC aptasensor is based on the formation of a selective complex between tropomyosin and immobilized aptamerTROP probe on the surface of ITO/g-C3N4-TiO2/PEI/aptamerTROP-Ru(NH3)6+3. After the incubation of tropomyosin with TROP aptamer probe, the photocurrent signal decreased due to releasing adsorbed Ru(NH3)63+ on aptamer and preventing AA from scavenging photogenerated holes to the photoactive modified electrode. Under the optimized conditions, the fabricated PEC aptasensor was used for the determination of shrimp tropomyosin in the concentration range of 1–400 ng mL−1 with a limit of detection of 0.23 ng mL−1. The proposed PEC aptasensor exhibited high selectivity, sensitivity, and good stability. © 2017 Elsevier B.V.