Drug Sensitivity Profile of Fungi Isolated From Onychomycosis Patients and Evaluation of Squalene Epoxidase Mutation in One Terbinafine-Resistant Trichophyton Mentagrophytes Species

Drug Sensitivity Profile of Fungi Isolated From Onychomycosis Patients and Evaluation of Squalene Epoxidase Mutation in One Terbinafine-Resistant Trichophyton Mentagrophytes Species Publisher Pubmed

Firooz A¹ ; Daneshpazhooh M² ; Lotfali E³ ; Sharzad Kavkani M⁴ ; Ghasemi Z⁵ ; Khamesipoor A¹ ; Nassiri Kashani M¹ ; Miramin Mohammadi A¹ ; Skandari SE¹ ; Ahmad Nasrollahi S¹ ; Fattahi A¹

Show Affiliations

1. Center for Research and Training in Skin Disease and Leprosy, Tehran University of Medical Sciences, Tehran, 1416613675, Iran
2. Department of Dermatology, Razi Dermatology Hospital, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4. Department of Microbial Biotechnology, Islamic Azad University, Damghan, Iran
5. Razi Hospital, Tehran University of Medical Sciences, Tehran, Iran

Source: Microbial Drug Resistance Published:2021

Abstract

Background: The resistance to treatment of onychomycosis is increasingly reported. The present study aimed to assess the antifungal activity of itraconazole, terbinafine, luliconazole, and efinaconazole against dermatophytes, molds, and also yeast isolated from patients with onychomycosis. Furthermore, the mechanism of resistance to terbinafine in resistant Trichophyton mentagrophytes species was evaluated using the squalene epoxidase (SQLE) gene sequence. Methods: A total of 71 fungal isolates were collected from 97 patients with suspected onychomycosis. The identification of fungal species was performed using conventional and molecular approaches. In vitro drug susceptibility for itraconazole, terbinafine, luliconazole, and efinaconazole was carried out using the broth microdilution method according to the CLSI-M60 and CLSI-M38 3rd ed., respectively. The SQLE gene of one terbinafine-resistant T. mentagrophytes was amplified using the specific primers. Results: Efinaconazole and luliconazole demonstrated higher effectiveness against all isolates in the study. One mismatch was detected at position 1177, which showed A → C change associated with Phe397Leu amino acid substitution of the SQLE protein in terbinafine-resistant T. mentagrophytes. Conclusion: The occurrence of resistant strains of organisms causing onychomycosis should be considered and evaluated. Furthermore, the identification of amino acid changes responsible for resistance to antifungals is a useful consideration in drug-target interaction. © Copyright 2021, Mary Ann Liebert, Inc., publishers 2021.

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Experts (# of related papers)

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Alireza Firooz (5)

Mahsa Fattahi (4)

Style	Citing Format
MLA	Firooz A, et al.. "Drug Sensitivity Profile of Fungi Isolated From Onychomycosis Patients and Evaluation of Squalene Epoxidase Mutation in One Terbinafine-Resistant Trichophyton Mentagrophytes Species." Microbial Drug Resistance, vol. 27, no. 12, 2021, pp. 1658-1663.
APA	Firooz A, Daneshpazhooh M, Lotfali E, Sharzad Kavkani M, Ghasemi Z, Khamesipoor A, Nassiri Kashani M, Miramin Mohammadi A, Skandari SE, Ahmad Nasrollahi S, Fattahi A (2021). Drug Sensitivity Profile of Fungi Isolated From Onychomycosis Patients and Evaluation of Squalene Epoxidase Mutation in One Terbinafine-Resistant Trichophyton Mentagrophytes Species. Microbial Drug Resistance, 27(12), 1658-1663.
Chicago	Firooz A, Daneshpazhooh M, Lotfali E, Sharzad Kavkani M, Ghasemi Z, Khamesipoor A, Nassiri Kashani M, et al.. "Drug Sensitivity Profile of Fungi Isolated From Onychomycosis Patients and Evaluation of Squalene Epoxidase Mutation in One Terbinafine-Resistant Trichophyton Mentagrophytes Species." Microbial Drug Resistance 27, no. 12 (2021): 1658-1663.
Harvard	Firooz A et al. (2021) 'Drug Sensitivity Profile of Fungi Isolated From Onychomycosis Patients and Evaluation of Squalene Epoxidase Mutation in One Terbinafine-Resistant Trichophyton Mentagrophytes Species', Microbial Drug Resistance, 27(12), pp. 1658-1663.
Vancouver	Firooz A, Daneshpazhooh M, Lotfali E, Sharzad Kavkani M, Ghasemi Z, Khamesipoor A, et al.. Drug Sensitivity Profile of Fungi Isolated From Onychomycosis Patients and Evaluation of Squalene Epoxidase Mutation in One Terbinafine-Resistant Trichophyton Mentagrophytes Species. Microbial Drug Resistance. 2021;27(12):1658-1663.
BibTex	@article{ author = {Firooz A and Daneshpazhooh M and Lotfali E and Sharzad Kavkani M and Ghasemi Z and Khamesipoor A and Nassiri Kashani M and Miramin Mohammadi A and Skandari SE and Ahmad Nasrollahi S and Fattahi A}, title = {Drug Sensitivity Profile of Fungi Isolated From Onychomycosis Patients and Evaluation of Squalene Epoxidase Mutation in One Terbinafine-Resistant Trichophyton Mentagrophytes Species}, journal = {Microbial Drug Resistance}, volume = {27}, number = {12}, pages = {1658-1663}, year = {2021} }
RIS	TY - JOUR AU - Firooz A AU - Daneshpazhooh M AU - Lotfali E AU - Sharzad Kavkani M AU - Ghasemi Z AU - Khamesipoor A AU - Nassiri Kashani M AU - Miramin Mohammadi A AU - Skandari SE AU - Ahmad Nasrollahi S AU - Fattahi A TI - Drug Sensitivity Profile of Fungi Isolated From Onychomycosis Patients and Evaluation of Squalene Epoxidase Mutation in One Terbinafine-Resistant Trichophyton Mentagrophytes Species JO - Microbial Drug Resistance VL - 27 IS - 12 SP - 1658 EP - 1663 PY - 2021 ER -

Style

Citing Format

MLA

Firooz A, et al.. "Drug Sensitivity Profile of Fungi Isolated From Onychomycosis Patients and Evaluation of Squalene Epoxidase Mutation in One Terbinafine-Resistant Trichophyton Mentagrophytes Species." Microbial Drug Resistance, vol. 27, no. 12, 2021, pp. 1658-1663.

APA

Firooz A, Daneshpazhooh M, Lotfali E, Sharzad Kavkani M, Ghasemi Z, Khamesipoor A, Nassiri Kashani M, Miramin Mohammadi A, Skandari SE, Ahmad Nasrollahi S, Fattahi A (2021). Drug Sensitivity Profile of Fungi Isolated From Onychomycosis Patients and Evaluation of Squalene Epoxidase Mutation in One Terbinafine-Resistant Trichophyton Mentagrophytes Species. Microbial Drug Resistance, 27(12), 1658-1663.

Chicago

Firooz A, Daneshpazhooh M, Lotfali E, Sharzad Kavkani M, Ghasemi Z, Khamesipoor A, Nassiri Kashani M, et al.. "Drug Sensitivity Profile of Fungi Isolated From Onychomycosis Patients and Evaluation of Squalene Epoxidase Mutation in One Terbinafine-Resistant Trichophyton Mentagrophytes Species." Microbial Drug Resistance 27, no. 12 (2021): 1658-1663.

Harvard

Firooz A et al. (2021) 'Drug Sensitivity Profile of Fungi Isolated From Onychomycosis Patients and Evaluation of Squalene Epoxidase Mutation in One Terbinafine-Resistant Trichophyton Mentagrophytes Species', Microbial Drug Resistance, 27(12), pp. 1658-1663.

Vancouver

Firooz A, Daneshpazhooh M, Lotfali E, Sharzad Kavkani M, Ghasemi Z, Khamesipoor A, et al.. Drug Sensitivity Profile of Fungi Isolated From Onychomycosis Patients and Evaluation of Squalene Epoxidase Mutation in One Terbinafine-Resistant Trichophyton Mentagrophytes Species. Microbial Drug Resistance. 2021;27(12):1658-1663.

BibTex

@article{ author = {Firooz A and Daneshpazhooh M and Lotfali E and Sharzad Kavkani M and Ghasemi Z and Khamesipoor A and Nassiri Kashani M and Miramin Mohammadi A and Skandari SE and Ahmad Nasrollahi S and Fattahi A}, title = {Drug Sensitivity Profile of Fungi Isolated From Onychomycosis Patients and Evaluation of Squalene Epoxidase Mutation in One Terbinafine-Resistant Trichophyton Mentagrophytes Species}, journal = {Microbial Drug Resistance}, volume = {27}, number = {12}, pages = {1658-1663}, year = {2021} }

RIS

TY - JOUR
AU - Firooz A
AU - Daneshpazhooh M
AU - Lotfali E
AU - Sharzad Kavkani M
AU - Ghasemi Z
AU - Khamesipoor A
AU - Nassiri Kashani M
AU - Miramin Mohammadi A
AU - Skandari SE
AU - Ahmad Nasrollahi S
AU - Fattahi A
TI - Drug Sensitivity Profile of Fungi Isolated From Onychomycosis Patients and Evaluation of Squalene Epoxidase Mutation in One Terbinafine-Resistant Trichophyton Mentagrophytes Species
JO - Microbial Drug Resistance
VL - 27
IS - 12
SP - 1658
EP - 1663
PY - 2021
ER -

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