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Bio-Conjugation of Anti-Human Cd3 Monoclonal Antibodies to Magnetic Nanoparticles by Using Cyanogen Bromide: A Potential for Cell Sorting and Noninvasive Diagnosis Publisher Pubmed



Moradi N1 ; Muhammadnejad S2 ; Delavari H3 ; Pournoori N4, 5 ; Oghabian MA5 ; Ghafouri H6, 7
Authors
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Authors Affiliations
  1. 1. Department of Biochemistry, University of Guilan, Rasht, Iran
  2. 2. Gene Therapy Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Materials Engineering Department, Tarbiat Modares University, Iran
  4. 4. Biomarker Imaging and Analysis Group, Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Iran
  5. 5. Department of Medical Physics and Biomedical Engineering, School of Medicine, Tehran University of Medical Science, Tehran, Iran
  6. 6. Department of Biochemistry, Faculty of Science, University of Guilan, Rasht, Iran
  7. 7. Department of Marine Sciences, The Caspian Sea Basin Research Center, University of Guilan, Rasht, Iran

Source: International Journal of Biological Macromolecules Published:2021


Abstract

The conjugation of monoclonal antibodies with superparamagnetic iron oxide nanoparticles (SPIONs) has appeared as a potential multifunctional clinical tool, which can effectively diagnose cancers and monitor their treatment, specifically. Despite the presence of different methods for conjugating antibodies to iron oxide nanoparticles, novel cost-effective and simpler conjugation techniques should be performed in this regard. In current study, an anti-CD3 monoclonal antibody was conjugated to the Fe3O4 coated by carboxymethyl dextran (CMD) using cyanogen bromide (CNBr). Moreover, EDC/NHS techniques were applied as a positive control. The experimental results showed that the Conjugation was performed and the presence of the antibody conjugated to the MNPs in human xenograft tumors was confirmed using Prussian blue (PB) staining, following magnetic resonance imaging (MRI), 30 min after injection. This conjugation method was shown to be able to separate CD3+ T lymphocytes efficiently from whole blood with high purity. Accordingly, this type of bio-conjugation method can be utilized in the future for cell sorting, and can be applied for adopted cell therapies such as CAR-T cell (Chimeric antigen receptor T cell) therapy, as well as targeted MRI imaging. © 2021
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