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Surface Modification and Bioconjugation of Anti-Cd4 Monoclonal Antibody to Magnetic Nanoparticles As a Highly Efficient Affinity Adsorbent for Positive Selection of Peripheral Blood T Cd4+ Lymphocytes Publisher Pubmed



Rashid Z1 ; Shokri F1 ; Abbasi A2 ; Khoobi M3 ; Zarnani AH1, 4, 5
Authors
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Authors Affiliations
  1. 1. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. School of Chemistry, College of Science, University of Tehran, Tehran, Iran
  3. 3. Department of Pharmaceutical Biomaterials, Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
  5. 5. Immunology Research Center (IRC), Iran University of Medical Sciences, Tehran, Iran

Source: International Journal of Biological Macromolecules Published:2020


Abstract

Magnetic activated cell sorting (MACS) is a straightforward and time-saving procedure for isolation of different healthy functional cells. The present study aimed for the developing of a new MACS-based platform for isolation of peripheral blood T CD4+ lymphocytes. For this goal, first: Fe3O4 magnetic nanoparticles (MNP) were prepared by co-precipitation of Fe (III) and Fe (II) ions and then coated by SiO2 shell, followed by the grafting of N-(phosphonomethyl) iminodiacetic acid (PMIDA) on the surface of fabricated MNP, Fe3O4@SiO2@PMIDA were formed. These MNP were further tested for their ability to bind CD4 T lymphocytes. Through conjugation of the anti-CD4 monoclonal antibody on the surface of Fe3O4@SiO2@PMIDA MNP. The newly developed immunomagnetic particles efficiently isolated T CD4+ lymphocytes from whole blood with high purity Therefore, our MNP afford an efficient tool for the cell separation process and further present the dramatic potential to be applied to other areas of biomedical application. © 2020
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