Preparation and Evaluation of 68Ga-Ecc As a Pet Renal Imaging Agent

Preparation and Evaluation of 68Ga-Ecc As a Pet Renal Imaging Agent Publisher

Mirzaei A¹ ; Jalilian AR¹ ; Aghanejad A¹ ; Mazidi M¹ ; Yousefnia H¹ ; Shabani G¹ ; Ardaneh K¹ ; Geramifar P² ; Beiki D²

Show Affiliations

1. Radiation Application Research School, Nuclear Science and Technology Research Institute, Tehran, 141551339, Iran
2. Research Center for Nuclear Medicine, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

Source: Nuclear Medicine and Molecular Imaging Published:2015

Abstract

Purpose: Development of a gallium-68-labeled renal tracer can be a good substitute for Tc-99m, a known SPECT tracer. In this study, effort was made to develop 68Ga-ethylenecysteamine cysteine (68Ga-ECC). Methods: Ga-ECC was prepared using generator-based 68GaCl3 and ethylenecysteamine cysteine (ECC) at optimized conditions. Stability of the complex was checked in human serum followed by partition coefficient determination of the tracer. The biodistribution of the tracer in rats was studied using tissue counting and PET/CT imaging up to 120 min. Results: Ga-ECC was prepared at optimized conditions in 15 min at 90 °C (radiochemical purity ≈97 ± 0.88 % ITLC, >99 % HPLC, specific activity: 210 ± 5 GBq/mM). 68Ga-ECC was a water-soluble complex based on partition coefficient data (log P; −1.378) and was stable in the presence of human serum for 2 h at 37 °C. The biodistribution of the tracer demonstrated high kidney excretion of the tracer in 10–20 min. The SUVmax ratios of the liver to left kidney were 0.38 and 0.39 for 30 and 90 min, respectively, indicating high kidney uptake. Conclusion: Initial biodistribution results showed significant kidney and urinary excretion of the tracer comparable to that of the homologous 99mTc compound. The complex could be a possible PET kidney imaging agent with a fast imaging time. © 2015, Korean Society of Nuclear Medicine.

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Style	Citing Format
MLA	Mirzaei A, et al.. "Preparation and Evaluation of 68Ga-Ecc As a Pet Renal Imaging Agent." Nuclear Medicine and Molecular Imaging, vol. 49, no. 3, 2015, pp. 208-216.
APA	Mirzaei A, Jalilian AR, Aghanejad A, Mazidi M, Yousefnia H, Shabani G, Ardaneh K, Geramifar P, Beiki D (2015). Preparation and Evaluation of 68Ga-Ecc As a Pet Renal Imaging Agent. Nuclear Medicine and Molecular Imaging, 49(3), 208-216.
Chicago	Mirzaei A, Jalilian AR, Aghanejad A, Mazidi M, Yousefnia H, Shabani G, Ardaneh K, Geramifar P, Beiki D. "Preparation and Evaluation of 68Ga-Ecc As a Pet Renal Imaging Agent." Nuclear Medicine and Molecular Imaging 49, no. 3 (2015): 208-216.
Harvard	Mirzaei A et al. (2015) 'Preparation and Evaluation of 68Ga-Ecc As a Pet Renal Imaging Agent', Nuclear Medicine and Molecular Imaging, 49(3), pp. 208-216.
Vancouver	Mirzaei A, Jalilian AR, Aghanejad A, Mazidi M, Yousefnia H, Shabani G, et al.. Preparation and Evaluation of 68Ga-Ecc As a Pet Renal Imaging Agent. Nuclear Medicine and Molecular Imaging. 2015;49(3):208-216.
BibTex	@article{ author = {Mirzaei A and Jalilian AR and Aghanejad A and Mazidi M and Yousefnia H and Shabani G and Ardaneh K and Geramifar P and Beiki D}, title = {Preparation and Evaluation of 68Ga-Ecc As a Pet Renal Imaging Agent}, journal = {Nuclear Medicine and Molecular Imaging}, volume = {49}, number = {3}, pages = {208-216}, year = {2015} }
RIS	TY - JOUR AU - Mirzaei A AU - Jalilian AR AU - Aghanejad A AU - Mazidi M AU - Yousefnia H AU - Shabani G AU - Ardaneh K AU - Geramifar P AU - Beiki D TI - Preparation and Evaluation of 68Ga-Ecc As a Pet Renal Imaging Agent JO - Nuclear Medicine and Molecular Imaging VL - 49 IS - 3 SP - 208 EP - 216 PY - 2015 ER -

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