Long Noncoding Rna Linc00978 Acts As a Potential Diagnostic Biomarker in Patients With Colorectal Cancer Publisher Pubmed



Ghasemian M^{1, 2} ; Rajabibazl M² ; Mirfakhraie R^{3, 4} ; Razavi AE⁵ ; Sadeghi H⁶ Show Affiliations
Source: Experimental and Molecular Pathology Published:2021

Abstract

Colorectal cancer (CRC) as a lethal malignancy has been associated with dysregulation of several genes and pathways. Long noncoding RNAs (lncRNAs) play an important role in gene expression regulation. In the current research, we aim to evaluate the expression of LINC00978 in CRC samples and adjacent tissues. Using Quantitative Real-Time PCR (qRT-PCR) method, we assessed the expression levels of LINC00978 and β-catenin in 70 pairs of CRC and adjacent tissues. Moreover, the association between clinicopathological features and the LINC00978 expression levels was investigated. To assess the diagnostic power of LINC00978 expression in CRC, receiver operating characteristic (ROC) curve was plotted. The relationship between LINC00978 and β-catenin expression levels was evaluated using correlation analysis. A markedly increased level of LINC00978 and β-catenin expression levels was observed in CRC samples compared with adjacent tissues (P < 0.0001). No significant association was detected between LINC00978 expression level and the patient's clinicopathological features. The results of Pearson's correlation coefficient highlighted a positive correlation between LINC00978 and β-catenin expression (r2 = 0.4695, P < 0.0001). According to the area under curve (AUC) value, LINC00978 expression differentiates CRC samples from the adjacent tissues (AUC = 0.81, P < 0.0001). The present results suggest that LINC00978 may play a critical role in CRC progression via Wnt pathway. The potential role of LINC00978 as a diagnostic biomarker needs to be further investigated in future studies. © 2021 Elsevier Inc.